Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects

Detalhes bibliográficos
Autor(a) principal: Oliveira, D. A.
Data de Publicação: 2008
Outros Autores: Harasawa, C., Seibert, C. S., Silva, L. L. Casais e, Pimenta, D. C., Lebrun, I., Sandoval, M. R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6346
Resumo: Acesso restrito: Texto completo. p. 629-639.
id UFBA-2_b7b53ee9d0fa01d0f3791f0cc1329ba0
oai_identifier_str oai:repositorio.ufba.br:ri/6346
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Oliveira, D. A.Harasawa, C.Seibert, C. S.Silva, L. L. Casais ePimenta, D. C.Lebrun, I.Sandoval, M. R. L.Oliveira, D. A.Harasawa, C.Seibert, C. S.Silva, L. L. Casais ePimenta, D. C.Lebrun, I.Sandoval, M. R. L.2012-07-05T18:41:58Z20080361-9230http://www.repositorio.ufba.br/ri/handle/ri/6346v. 75, n. 5Acesso restrito: Texto completo. p. 629-639.The present study evaluated four phospholipase A2 (PLA2) (Mlx-8, Mlx-9, Mlx-11 and Mlx-12) isolated from Micrurus lemniscatus snake venom (Elapidae). The effects of intrahippocampal administration of these toxins have been determined on behavior, electroencephalography, and neuronal degeneration in rats. These four PLA2 toxins induced motor and EEG alterations in a dose-dependent manner. Behavioral convulsions were characterized by clonic movements and were often accompanied by EEG alterations. Mlx toxins were convulsive but weakly epileptogenic, since low rates of seizure discharges were observed in EEG records. Neuronal injury seemed to depend on the dose of the toxin used. The highest doses of toxins caused severe intoxication and death of some animals. The injury of hippocampal cells was characterized by massive neuronal loss and hippocampal gliosis. A high occurrence of compulsive scratching was observed. Moreover, the onset of seizures induced by Mlx toxins was markedly delayed. The similarities between the effects of Mlx PLA2s and those isolated from other Elapidae snakes venoms suggest that their toxicity are probably due to their specific binding to neuronal membranes and to the catalysis of phospholipid hydrolysis, producing lysophospholipids and fatty acids. These compounds likely disturb the membrane conformation, causing a marked increase in the release of neurotransmitters and concurrent inhibition of vesicle fission and recycling. These toxins can be a useful tool to investigate properties of endogenous secretory PLA2s and therefore may be important both to study mechanisms involved in neurotransmitter release at nerve terminals and to explain the convulsive properties of PLA2s toxins.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-07-05T18:41:58Z No. of bitstreams: 1 __ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf: 488922 bytes, checksum: cb9fdf7f4710493054f8eea9685265ca (MD5)Made available in DSpace on 2012-07-05T18:41:58Z (GMT). No. of bitstreams: 1 __ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf: 488922 bytes, checksum: cb9fdf7f4710493054f8eea9685265ca (MD5) Previous issue date: 2008http://dx.doi.org/10.1016/j.brainresbull.2007.10.007reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAElapidae venom PLA2Micrurus lemniscatus toxinsHippocampal injurySeizureEEGNeurotoxicityPhospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspectsBRAIN RESEARCH BULLETINinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdfapplication/pdf488922https://repositorio.ufba.br/bitstream/ri/6346/1/__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdfcb9fdf7f4710493054f8eea9685265caMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6346/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf.txt__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf.txtExtracted texttext/plain53614https://repositorio.ufba.br/bitstream/ri/6346/3/__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf.txt2069968c0beaa649a1f128ac81fc662aMD53ri/63462022-07-05 14:03:12.206oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:12Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
dc.title.alternative.pt_BR.fl_str_mv BRAIN RESEARCH BULLETIN
title Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
spellingShingle Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
Oliveira, D. A.
Elapidae venom PLA2
Micrurus lemniscatus toxins
Hippocampal injury
Seizure
EEG
Neurotoxicity
title_short Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
title_full Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
title_fullStr Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
title_full_unstemmed Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
title_sort Phospholipases A2 isolated from Micrurus lemniscatus coral snake venom: Behavioral, electroencephalographic, and neuropathological aspects
author Oliveira, D. A.
author_facet Oliveira, D. A.
Harasawa, C.
Seibert, C. S.
Silva, L. L. Casais e
Pimenta, D. C.
Lebrun, I.
Sandoval, M. R. L.
author_role author
author2 Harasawa, C.
Seibert, C. S.
Silva, L. L. Casais e
Pimenta, D. C.
Lebrun, I.
Sandoval, M. R. L.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, D. A.
Harasawa, C.
Seibert, C. S.
Silva, L. L. Casais e
Pimenta, D. C.
Lebrun, I.
Sandoval, M. R. L.
Oliveira, D. A.
Harasawa, C.
Seibert, C. S.
Silva, L. L. Casais e
Pimenta, D. C.
Lebrun, I.
Sandoval, M. R. L.
dc.subject.por.fl_str_mv Elapidae venom PLA2
Micrurus lemniscatus toxins
Hippocampal injury
Seizure
EEG
Neurotoxicity
topic Elapidae venom PLA2
Micrurus lemniscatus toxins
Hippocampal injury
Seizure
EEG
Neurotoxicity
description Acesso restrito: Texto completo. p. 629-639.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-07-05T18:41:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6346
dc.identifier.issn.none.fl_str_mv 0361-9230
dc.identifier.number.pt_BR.fl_str_mv v. 75, n. 5
identifier_str_mv 0361-9230
v. 75, n. 5
url http://www.repositorio.ufba.br/ri/handle/ri/6346
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.brainresbull.2007.10.007
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/6346/1/__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf
https://repositorio.ufba.br/bitstream/ri/6346/2/license.txt
https://repositorio.ufba.br/bitstream/ri/6346/3/__ac.els-cdn.com_S036192...2b000234b98269da6c8e755909d5c.pdf.txt
bitstream.checksum.fl_str_mv cb9fdf7f4710493054f8eea9685265ca
1b89a9a0548218172d7c829f87a0eab9
2069968c0beaa649a1f128ac81fc662a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459403261640704

Registros relacionados