Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Maria Zoraida Daltro de
Data de Publicação: 2011
Outros Autores: Vale, Vera, Keid, Lara, Freire, Songeli Menezes, Nascimento, Roberto José Meyer, Portela, Ricardo Wagner Dias, Melo, Stella Maria Barrouin
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5306
Resumo: Acesso restrito: Texto completo. p. 425-431.
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spelling Oliveira, Maria Zoraida Daltro deVale, VeraKeid, LaraFreire, Songeli MenezesNascimento, Roberto José MeyerPortela, Ricardo Wagner DiasMelo, Stella Maria BarrouinOliveira, Maria Zoraida Daltro deVale, VeraKeid, LaraFreire, Songeli MenezesNascimento, Roberto José MeyerPortela, Ricardo Wagner DiasMelo, Stella Maria Barrouin2012-02-06T18:13:57Z20110034-5288http://www.repositorio.ufba.br/ri/handle/ri/5306v. 90, n. 3.Acesso restrito: Texto completo. p. 425-431.In the present study, the validation of an enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of canine brucellosis is described. Two different antigenic extracts, obtained by heat or ultrasonic homogenization of microbial antigens from a wild isolate of Brucella canis bacteria, were compared by ELISA and Western blot (WB). A total of 145 canine sera were used to define sensitivity, specificity and accuracy of the ELISA as follows: (1) sera from 34 animals with natural B. canis infection, confirmed by blood culture and PCR, as well as 51 sera samples from healthy dogs with negative results by the agar–gel immunodiffusion (AGID) test for canine brucellosis, were used as the control panel for B. canis infection; and (2) to scrutinize the possibility of cross reactions with other common dog infections in the same geographical area in Brazil, 60 sera samples from dogs harboring known infections by Leptospira sp., Ehrlichia canis, canine distemper virus (CDV), Neospora caninum, Babesia canis and Leishmania chagasi (10 in each group) were included in the study. The ELISA using heat soluble bacterial extract (HE-antigen) as antigen showed the best values of sensitivity (91.18%), specificity (100%) and accuracy (96.47%). In the WB analyses, the HE-antigen showed no cross-reactivity with sera from dogs with different infections, while the B. canis sonicate had various protein bands identified by those sera. The performance of the ELISA standardized with the heat soluble B. canis antigen indicates that this assay can be used as a reliable and practical method to confirm infection by this microorganism, as well as a tool for seroepidemiological studies.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-02-06T18:13:57Z No. of bitstreams: 1 __pdn.sciencedirect.com.....0-S0034528810002407-main.pdf: 209146 bytes, checksum: 2899617f88a27e7bbac8c23e41fa202f (MD5)Made available in DSpace on 2012-02-06T18:13:57Z (GMT). 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dc.title.pt_BR.fl_str_mv Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
dc.title.alternative.pt_BR.fl_str_mv Research in veterinary science
title Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
spellingShingle Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
Oliveira, Maria Zoraida Daltro de
Brucella canis
Canine Brucellosis
ELISA
Validation
Serology
Diagnosis
title_short Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
title_full Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
title_fullStr Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
title_full_unstemmed Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
title_sort Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis
author Oliveira, Maria Zoraida Daltro de
author_facet Oliveira, Maria Zoraida Daltro de
Vale, Vera
Keid, Lara
Freire, Songeli Menezes
Nascimento, Roberto José Meyer
Portela, Ricardo Wagner Dias
Melo, Stella Maria Barrouin
author_role author
author2 Vale, Vera
Keid, Lara
Freire, Songeli Menezes
Nascimento, Roberto José Meyer
Portela, Ricardo Wagner Dias
Melo, Stella Maria Barrouin
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Maria Zoraida Daltro de
Vale, Vera
Keid, Lara
Freire, Songeli Menezes
Nascimento, Roberto José Meyer
Portela, Ricardo Wagner Dias
Melo, Stella Maria Barrouin
Oliveira, Maria Zoraida Daltro de
Vale, Vera
Keid, Lara
Freire, Songeli Menezes
Nascimento, Roberto José Meyer
Portela, Ricardo Wagner Dias
Melo, Stella Maria Barrouin
dc.subject.por.fl_str_mv Brucella canis
Canine Brucellosis
ELISA
Validation
Serology
Diagnosis
topic Brucella canis
Canine Brucellosis
ELISA
Validation
Serology
Diagnosis
description Acesso restrito: Texto completo. p. 425-431.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-06T18:13:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5306
dc.identifier.issn.none.fl_str_mv 0034-5288
dc.identifier.number.pt_BR.fl_str_mv v. 90, n. 3.
identifier_str_mv 0034-5288
v. 90, n. 3.
url http://www.repositorio.ufba.br/ri/handle/ri/5306
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.rvsc.2010.07.004
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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