Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1998 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/8286 |
Resumo: | Texto completo: acesso restrito. p.2074-2078 |
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Fragoso, Maria Candida B. VillaresLatronico, Ana ClaudiaCarvalho, Filomena MarinoZerbini, Maria Claudia N.Marcondes, Jose Antonio MiguelAraujo, Leila M. B.Lando, Valeria S.Frazzatto, Eliana T.Mendonca, Berenice B.Villares, Sandra Mara F.Fragoso, Maria Candida B. VillaresLatronico, Ana ClaudiaCarvalho, Filomena MarinoZerbini, Maria Claudia N.Marcondes, Jose Antonio MiguelAraujo, Leila M. B.Lando, Valeria S.Frazzatto, Eliana T.Mendonca, Berenice B.Villares, Sandra Mara F.2013-01-31T13:00:40Z19980021-972Xhttp://www.repositorio.ufba.br/ri/handle/ri/8286v. 83, n. 6Texto completo: acesso restrito. p.2074-2078Activating mutations of the G protein genes have been associated with the development of several endocrine neoplasms. Such activating mutations, gip2, affecting the α-subunit of the Gαi2 protein were previously described by a single group in 30% of ovarian sex cord stromal tumors. Other activating mutations of the α-subunit of the Gs (gsp) have been identified in GH-secreting and nonfunctioning pituitary tumors, autonomous thyroid adenomas, and all affected McCune-Albright tissues, but not in sex cord stromal tumors. In the present study, we investigated the presence of gip2 and gsp mutations in 14 human sex cord stromal tumors. Six Leydig cell tumors (4 ovaries and 2 testes), 2 thecomas, 2 granulosa cell tumors, 3 androblastomas, and 1 gonadoblastoma (sex cord and germ cell) were included in this study. Genomic DNA was obtained from either fresh-frozen tumor tissues or paraffin-embedded sections and in some cases from blood samples. Using PCR, denaturing gradient gel electrophoresis, and direct sequencing, we detected 4 tumors (66.6%) with the gsp mutation (R201C) in our series of ovarian and testicular Leydig cell tumors. In contrast, no gip2 mutations were found in any of the sex cord stromal tumors studied. In conclusion, our findings suggest that the putative oncogene gsp may play a significant role in the molecular mechanism of these tumors.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2013-01-30T13:43:52Z No. of bitstreams: 1 Fragoso.pdf: 982718 bytes, checksum: 057e6284017c9d1b55d415d7986a14c0 (MD5)Approved for entry into archive by Fatima Cleômenis Botelho Maria (botelho@ufba.br) on 2013-01-31T13:00:40Z (GMT) No. of bitstreams: 1 Fragoso.pdf: 982718 bytes, checksum: 057e6284017c9d1b55d415d7986a14c0 (MD5)Made available in DSpace on 2013-01-31T13:00:40Z (GMT). No. of bitstreams: 1 Fragoso.pdf: 982718 bytes, checksum: 057e6284017c9d1b55d415d7986a14c0 (MD5) Previous issue date: 1998http://dx.doi.org/10.1210/jc.83.6.2074reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAActivating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumorsJournal of Clinical Endocrinology and Metabolisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINALFragoso.pdfFragoso.pdfapplication/pdf982718https://repositorio.ufba.br/bitstream/ri/8286/1/Fragoso.pdf057e6284017c9d1b55d415d7986a14c0MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/8286/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTFragoso.pdf.txtFragoso.pdf.txtExtracted texttext/plain25077https://repositorio.ufba.br/bitstream/ri/8286/3/Fragoso.pdf.txt414f2c5e9b0da9cdf6d68f448cd50113MD53ri/82862022-07-05 14:03:03.205oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:03Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Clinical Endocrinology and Metabolism |
| title |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
| spellingShingle |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors Fragoso, Maria Candida B. Villares |
| title_short |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
| title_full |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
| title_fullStr |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
| title_full_unstemmed |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
| title_sort |
Activating mutation of the stimulatory G protein (gsp) as a putative cause of ovarian and testicular human stromal Leydig cell tumors |
| author |
Fragoso, Maria Candida B. Villares |
| author_facet |
Fragoso, Maria Candida B. Villares Latronico, Ana Claudia Carvalho, Filomena Marino Zerbini, Maria Claudia N. Marcondes, Jose Antonio Miguel Araujo, Leila M. B. Lando, Valeria S. Frazzatto, Eliana T. Mendonca, Berenice B. Villares, Sandra Mara F. |
| author_role |
author |
| author2 |
Latronico, Ana Claudia Carvalho, Filomena Marino Zerbini, Maria Claudia N. Marcondes, Jose Antonio Miguel Araujo, Leila M. B. Lando, Valeria S. Frazzatto, Eliana T. Mendonca, Berenice B. Villares, Sandra Mara F. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fragoso, Maria Candida B. Villares Latronico, Ana Claudia Carvalho, Filomena Marino Zerbini, Maria Claudia N. Marcondes, Jose Antonio Miguel Araujo, Leila M. B. Lando, Valeria S. Frazzatto, Eliana T. Mendonca, Berenice B. Villares, Sandra Mara F. Fragoso, Maria Candida B. Villares Latronico, Ana Claudia Carvalho, Filomena Marino Zerbini, Maria Claudia N. Marcondes, Jose Antonio Miguel Araujo, Leila M. B. Lando, Valeria S. Frazzatto, Eliana T. Mendonca, Berenice B. Villares, Sandra Mara F. |
| description |
Texto completo: acesso restrito. p.2074-2078 |
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1998 |
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1998 |
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2013-01-31T13:00:40Z |
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info:eu-repo/semantics/publishedVersion |
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0021-972X |
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v. 83, n. 6 |
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0021-972X v. 83, n. 6 |
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