Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/4861 |
Resumo: | p.35-39 |
id |
UFBA-2_c5c63ee8eb3103ad882a2ae56650835a |
---|---|
oai_identifier_str |
oai:repositorio.ufba.br:ri/4861 |
network_acronym_str |
UFBA-2 |
network_name_str |
Repositório Institucional da UFBA |
repository_id_str |
1932 |
spelling |
Serravalle, KarinaSantos, NormaSardi, Silvia InêsSilva, Sarah Peregrino SantosRibeiro Junior, Hugo da CostaMattos, Ângela Peixoto deCampos, Gubio SoaresSerravalle, KarinaSantos, NormaSardi, Silvia InêsSilva, Sarah Peregrino SantosRibeiro Junior, Hugo da CostaMattos, Ângela Peixoto deCampos, Gubio Soares2011-12-14T16:35:07Z2011-12-14T16:35:07Z2007-02http://www.repositorio.ufba.br/ri/handle/ri/486111(1):35-39p.35-39Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed that the genotype G9 was present in 96,8% of the analyzed samples, in the second year, it was responsible for 96% and in the third year, 88,1%. The characterization of genotypes P demonstrated that the genotype P1A[8] was the most outstanding in all years. In this study we discuss the benefit to control the genotypes of Rotavirus through the molecular characterization for the development of potential vaccines.Submitted by Rigaud Andréa (andrearigaud16@yahoo.com.br) on 2011-12-14T16:35:07Z No. of bitstreams: 1 10.pdf: 219118 bytes, checksum: 4ff945a26f0e7f15ccf2f868c7dedb9e (MD5)Made available in DSpace on 2011-12-14T16:35:07Z (GMT). No. of bitstreams: 1 10.pdf: 219118 bytes, checksum: 4ff945a26f0e7f15ccf2f868c7dedb9e (MD5) Previous issue date: 2007-02RotavirusPCRgenotypechildrenMolecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, BrazilThe Brazilian Journal of Infectious Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINAL10.pdf10.pdfapplication/pdf219118https://repositorio.ufba.br/bitstream/ri/4861/1/10.pdf4ff945a26f0e7f15ccf2f868c7dedb9eMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/4861/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXT10.pdf.txt10.pdf.txtExtracted texttext/plain25048https://repositorio.ufba.br/bitstream/ri/4861/3/10.pdf.txt43e3acb00eebbc752198486a009b7e5fMD53ri/48612022-07-05 14:03:16.895oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:16Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil |
dc.title.alternative.pt_BR.fl_str_mv |
The Brazilian Journal of Infectious Diseases |
title |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil |
spellingShingle |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil Serravalle, Karina Rotavirus PCR genotype children |
title_short |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil |
title_full |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil |
title_fullStr |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil |
title_full_unstemmed |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil |
title_sort |
Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil |
author |
Serravalle, Karina |
author_facet |
Serravalle, Karina Santos, Norma Sardi, Silvia Inês Silva, Sarah Peregrino Santos Ribeiro Junior, Hugo da Costa Mattos, Ângela Peixoto de Campos, Gubio Soares |
author_role |
author |
author2 |
Santos, Norma Sardi, Silvia Inês Silva, Sarah Peregrino Santos Ribeiro Junior, Hugo da Costa Mattos, Ângela Peixoto de Campos, Gubio Soares |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Serravalle, Karina Santos, Norma Sardi, Silvia Inês Silva, Sarah Peregrino Santos Ribeiro Junior, Hugo da Costa Mattos, Ângela Peixoto de Campos, Gubio Soares Serravalle, Karina Santos, Norma Sardi, Silvia Inês Silva, Sarah Peregrino Santos Ribeiro Junior, Hugo da Costa Mattos, Ângela Peixoto de Campos, Gubio Soares |
dc.subject.por.fl_str_mv |
Rotavirus PCR genotype children |
topic |
Rotavirus PCR genotype children |
description |
p.35-39 |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-02 |
dc.date.accessioned.fl_str_mv |
2011-12-14T16:35:07Z |
dc.date.available.fl_str_mv |
2011-12-14T16:35:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/4861 |
dc.identifier.number.pt_BR.fl_str_mv |
11(1):35-39 |
url |
http://www.repositorio.ufba.br/ri/handle/ri/4861 |
identifier_str_mv |
11(1):35-39 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
instname_str |
Universidade Federal da Bahia (UFBA) |
instacron_str |
UFBA |
institution |
UFBA |
reponame_str |
Repositório Institucional da UFBA |
collection |
Repositório Institucional da UFBA |
bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/4861/1/10.pdf https://repositorio.ufba.br/bitstream/ri/4861/2/license.txt https://repositorio.ufba.br/bitstream/ri/4861/3/10.pdf.txt |
bitstream.checksum.fl_str_mv |
4ff945a26f0e7f15ccf2f868c7dedb9e 8bb3e3eb871f5854a9ec419f68651b60 43e3acb00eebbc752198486a009b7e5f |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
repository.mail.fl_str_mv |
|
_version_ |
1808459390398758912 |