Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil

Detalhes bibliográficos
Autor(a) principal: Serravalle, Karina
Data de Publicação: 2007
Outros Autores: Santos, Norma, Sardi, Silvia Inês, Silva, Sarah Peregrino Santos, Ribeiro Junior, Hugo da Costa, Mattos, Ângela Peixoto de, Campos, Gubio Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/4861
Resumo: p.35-39
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spelling Serravalle, KarinaSantos, NormaSardi, Silvia InêsSilva, Sarah Peregrino SantosRibeiro Junior, Hugo da CostaMattos, Ângela Peixoto deCampos, Gubio SoaresSerravalle, KarinaSantos, NormaSardi, Silvia InêsSilva, Sarah Peregrino SantosRibeiro Junior, Hugo da CostaMattos, Ângela Peixoto deCampos, Gubio Soares2011-12-14T16:35:07Z2011-12-14T16:35:07Z2007-02http://www.repositorio.ufba.br/ri/handle/ri/486111(1):35-39p.35-39Rotavirus is a major cause of infectious diarrhea in infants and young children. The objective of this study was to characterize the genotypes of Human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA in Salvador, Bahia, Brazil, during the years of 1999, 2000 and 2002. Fecal samples were analyzed (n=358) by methods EIARA and SDS-PAGE for detection of Rotavirus. Positive samples of one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR to determine genotypes G and P. A hundred sixty-eight (46.9%) samples were positive and 190 (53.1%) negative. Only 17 (4.7%) samples had divergent results. The distribution of genotypes G during the first year, showed that the genotype G9 was present in 96,8% of the analyzed samples, in the second year, it was responsible for 96% and in the third year, 88,1%. The characterization of genotypes P demonstrated that the genotype P1A[8] was the most outstanding in all years. In this study we discuss the benefit to control the genotypes of Rotavirus through the molecular characterization for the development of potential vaccines.Submitted by Rigaud Andréa (andrearigaud16@yahoo.com.br) on 2011-12-14T16:35:07Z No. of bitstreams: 1 10.pdf: 219118 bytes, checksum: 4ff945a26f0e7f15ccf2f868c7dedb9e (MD5)Made available in DSpace on 2011-12-14T16:35:07Z (GMT). No. of bitstreams: 1 10.pdf: 219118 bytes, checksum: 4ff945a26f0e7f15ccf2f868c7dedb9e (MD5) Previous issue date: 2007-02RotavirusPCRgenotypechildrenMolecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, BrazilThe Brazilian Journal of Infectious Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINAL10.pdf10.pdfapplication/pdf219118https://repositorio.ufba.br/bitstream/ri/4861/1/10.pdf4ff945a26f0e7f15ccf2f868c7dedb9eMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/4861/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXT10.pdf.txt10.pdf.txtExtracted texttext/plain25048https://repositorio.ufba.br/bitstream/ri/4861/3/10.pdf.txt43e3acb00eebbc752198486a009b7e5fMD53ri/48612022-07-05 14:03:16.895oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:16Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
dc.title.alternative.pt_BR.fl_str_mv The Brazilian Journal of Infectious Diseases
title Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
spellingShingle Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
Serravalle, Karina
Rotavirus
PCR
genotype
children
title_short Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
title_full Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
title_fullStr Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
title_full_unstemmed Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
title_sort Molecular Characterization of Group A Rotavirus Isolates Obtained from Hospitalized Children in Salvador, Bahia, Brazil
author Serravalle, Karina
author_facet Serravalle, Karina
Santos, Norma
Sardi, Silvia Inês
Silva, Sarah Peregrino Santos
Ribeiro Junior, Hugo da Costa
Mattos, Ângela Peixoto de
Campos, Gubio Soares
author_role author
author2 Santos, Norma
Sardi, Silvia Inês
Silva, Sarah Peregrino Santos
Ribeiro Junior, Hugo da Costa
Mattos, Ângela Peixoto de
Campos, Gubio Soares
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Serravalle, Karina
Santos, Norma
Sardi, Silvia Inês
Silva, Sarah Peregrino Santos
Ribeiro Junior, Hugo da Costa
Mattos, Ângela Peixoto de
Campos, Gubio Soares
Serravalle, Karina
Santos, Norma
Sardi, Silvia Inês
Silva, Sarah Peregrino Santos
Ribeiro Junior, Hugo da Costa
Mattos, Ângela Peixoto de
Campos, Gubio Soares
dc.subject.por.fl_str_mv Rotavirus
PCR
genotype
children
topic Rotavirus
PCR
genotype
children
description p.35-39
publishDate 2007
dc.date.issued.fl_str_mv 2007-02
dc.date.accessioned.fl_str_mv 2011-12-14T16:35:07Z
dc.date.available.fl_str_mv 2011-12-14T16:35:07Z
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dc.identifier.number.pt_BR.fl_str_mv 11(1):35-39
url http://www.repositorio.ufba.br/ri/handle/ri/4861
identifier_str_mv 11(1):35-39
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