Protic ionic liquid as additive on lipase immobilization using silica sol–gel
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/13809 |
Resumo: | Texto completo. Acesso restrito. p. 141-150 |
| id |
UFBA-2_c81ed62986d16b4d43a29cc86a4cd280 |
|---|---|
| oai_identifier_str |
oai:repositorio.ufba.br:ri/13809 |
| network_acronym_str |
UFBA-2 |
| network_name_str |
Repositório Institucional da UFBA |
| repository_id_str |
1932 |
| spelling |
Souza, Ranyere Lucena deFaria, Emanuelle Lima Pache deFigueiredo, Renan TavaresFreitas, Lisiane dos SantosDuro, Miguel Angel IglesiasSilva, Silvana Mattedi eZanin, Gisella MariaSantos, Onélia Aparecida Andreo dosCoutinho, João A. P.Lima, Álvaro SilvaSoares, Cleide Mara FariaSouza, Ranyere Lucena deFaria, Emanuelle Lima Pache deFigueiredo, Renan TavaresFreitas, Lisiane dos SantosDuro, Miguel Angel IglesiasSilva, Silvana Mattedi eZanin, Gisella MariaSantos, Onélia Aparecida Andreo dosCoutinho, João A. P.Lima, Álvaro SilvaSoares, Cleide Mara Faria2013-11-19T20:58:16Z20130141-0229http://repositorio.ufba.br/ri/handle/ri/13809v. 52, n. 3Texto completo. Acesso restrito. p. 141-150Ionic liquids (ILs) have evolved as a new type of non-aqueous solvents for biocatalysis, mainly due to their unique and tunable physical properties. A number of recent review papers have described a variety of enzymatic reactions conducted in IL solutions, on the other hand, to improve the enzyme’s activity and stability in ILs; major methods being explored include the enzyme immobilization (on solid support, sol–gel, etc.), protic ionic liquids used as an additive process. The immobilization of the lipase from Burkholderia cepacia by the sol–gel technique using protic ionic liquids (PIL) as additives to protect against inactivation of the lipase due to release of alcohol and shrinkage of the gel during the sol–gel process was investigated in this study. The influence of various factors such as the length of the alkyl chain of protic ionic liquids (monoethanolamine-based) and a concentration range between 0.5 and 3.0% (w/v) were evaluated. The resulting hydrophobic matrices and immobilized lipases were characterised with regard to specific surface area, adsorption–desorption isotherms, pore volume (Vp) and size (dp) according to nitrogen adsorption and scanning electron microscopy (SEM), physico-chemical properties (thermogravimetric – TG, differential scanning calorimetry – DSC and Fourier transform infrared spectroscopy – FTIR) and the potential for ethyl ester and emulsifier production. The total activity yields (Ya) for matrices of immobilized lipase employing protic ionic liquids as additives always resulted in higher values compared with the sample absent the protic ionic liquids, which represents 35-fold increase in recovery of enzymatic activity using the more hydrophobic protic ionic liquids. Compared with arrays of the immobilized biocatalyst without additive, in general, the immobilized biocatalyst in the presence of protic ionic liquids showed increased values of surface area (143–245 m2 g−1) and pore size (19–38 A˚ ). Immobilization with protic ionic liquids also favoured reduced mass loss according to TG curves (always less than 42.9%) when compared to the immobilized matrix without protic ionic liquids (45.1%), except for the sample containing 3.0% protic ionic liquids (46.5%), verified by thermogravimetric analysis. Ionic liquids containing a more hydrophobic alkyl group in the cationic moiety were beneficial for recovery of the activity of the immobilized lipase. The physico-chemical characterization confirmed the presence of the enzyme and its immobilized derivatives obtained in this study by identifying the presence of amino groups, and profiling enthalpy changes of mass loss.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-06-25T18:12:55Z No. of bitstreams: 1 252525mm.pdf: 886657 bytes, checksum: b7fabc55a8045a149328cf7dcb314890 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-19T20:58:16Z (GMT) No. of bitstreams: 1 252525mm.pdf: 886657 bytes, checksum: b7fabc55a8045a149328cf7dcb314890 (MD5)Made available in DSpace on 2013-11-19T20:58:16Z (GMT). No. of bitstreams: 1 252525mm.pdf: 886657 bytes, checksum: b7fabc55a8045a149328cf7dcb314890 (MD5) Previous issue date: 2013SalvadorEnzyme and Microbial TechnologyLipaseImmobilizationSol–gelProtic ionic liquidsProtic ionic liquid as additive on lipase immobilization using silica sol–gelEnzyme and Microbial Technologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAORIGINAL252525mm.pdf252525mm.pdfapplication/pdf886657https://repositorio.ufba.br/bitstream/ri/13809/1/252525mm.pdfb7fabc55a8045a149328cf7dcb314890MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13809/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT252525mm.pdf.txt252525mm.pdf.txtExtracted texttext/plain53725https://repositorio.ufba.br/bitstream/ri/13809/3/252525mm.pdf.txt0abc13d0169190176b8e80002b684ca9MD53ri/138092022-07-05 14:03:13.046oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:13Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel |
| dc.title.alternative.pt_BR.fl_str_mv |
Enzyme and Microbial Technology |
| title |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel |
| spellingShingle |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel Souza, Ranyere Lucena de Lipase Immobilization Sol–gel Protic ionic liquids |
| title_short |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel |
| title_full |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel |
| title_fullStr |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel |
| title_full_unstemmed |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel |
| title_sort |
Protic ionic liquid as additive on lipase immobilization using silica sol–gel |
| author |
Souza, Ranyere Lucena de |
| author_facet |
Souza, Ranyere Lucena de Faria, Emanuelle Lima Pache de Figueiredo, Renan Tavares Freitas, Lisiane dos Santos Duro, Miguel Angel Iglesias Silva, Silvana Mattedi e Zanin, Gisella Maria Santos, Onélia Aparecida Andreo dos Coutinho, João A. P. Lima, Álvaro Silva Soares, Cleide Mara Faria |
| author_role |
author |
| author2 |
Faria, Emanuelle Lima Pache de Figueiredo, Renan Tavares Freitas, Lisiane dos Santos Duro, Miguel Angel Iglesias Silva, Silvana Mattedi e Zanin, Gisella Maria Santos, Onélia Aparecida Andreo dos Coutinho, João A. P. Lima, Álvaro Silva Soares, Cleide Mara Faria |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Souza, Ranyere Lucena de Faria, Emanuelle Lima Pache de Figueiredo, Renan Tavares Freitas, Lisiane dos Santos Duro, Miguel Angel Iglesias Silva, Silvana Mattedi e Zanin, Gisella Maria Santos, Onélia Aparecida Andreo dos Coutinho, João A. P. Lima, Álvaro Silva Soares, Cleide Mara Faria Souza, Ranyere Lucena de Faria, Emanuelle Lima Pache de Figueiredo, Renan Tavares Freitas, Lisiane dos Santos Duro, Miguel Angel Iglesias Silva, Silvana Mattedi e Zanin, Gisella Maria Santos, Onélia Aparecida Andreo dos Coutinho, João A. P. Lima, Álvaro Silva Soares, Cleide Mara Faria |
| dc.subject.por.fl_str_mv |
Lipase Immobilization Sol–gel Protic ionic liquids |
| topic |
Lipase Immobilization Sol–gel Protic ionic liquids |
| description |
Texto completo. Acesso restrito. p. 141-150 |
| publishDate |
2013 |
| dc.date.accessioned.fl_str_mv |
2013-11-19T20:58:16Z |
| dc.date.issued.fl_str_mv |
2013 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/13809 |
| dc.identifier.issn.none.fl_str_mv |
0141-0229 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 52, n. 3 |
| identifier_str_mv |
0141-0229 v. 52, n. 3 |
| url |
http://repositorio.ufba.br/ri/handle/ri/13809 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Enzyme and Microbial Technology |
| publisher.none.fl_str_mv |
Enzyme and Microbial Technology |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
| instname_str |
Universidade Federal da Bahia (UFBA) |
| instacron_str |
UFBA |
| institution |
UFBA |
| reponame_str |
Repositório Institucional da UFBA |
| collection |
Repositório Institucional da UFBA |
| bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/13809/1/252525mm.pdf https://repositorio.ufba.br/bitstream/ri/13809/2/license.txt https://repositorio.ufba.br/bitstream/ri/13809/3/252525mm.pdf.txt |
| bitstream.checksum.fl_str_mv |
b7fabc55a8045a149328cf7dcb314890 1b89a9a0548218172d7c829f87a0eab9 0abc13d0169190176b8e80002b684ca9 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
| repository.mail.fl_str_mv |
|
| _version_ |
1808459449380110336 |