Distribution of 131I-labeled Bothrops erythromelas venom in mice

Detalhes bibliográficos
Autor(a) principal: Vasconcelos, C. M. L.
Data de Publicação: 1998
Outros Autores: Valença, R. C., Araújo, E. A., Modesto, J. C. A., Pontes, M. M., Brazil, Tania Kobler, Guarnieri, M. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7050
Resumo: p. 439-443
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spelling Vasconcelos, C. M. L.Valença, R. C.Araújo, E. A.Modesto, J. C. A.Pontes, M. M.Brazil, Tania KoblerGuarnieri, M. C.Vasconcelos, C. M. L.Valença, R. C.Araújo, E. A.Modesto, J. C. A.Pontes, M. M.Brazil, Tania KoblerGuarnieri, M. C.2012-10-24T18:45:51Z2012-10-24T18:45:51Z19981414-431Xhttp://www.repositorio.ufba.br/ri/handle/ri/7050v. 31, n. 3p. 439-443Bothrops erythromelas is responsible for many snake bites in northeastern Brazil. In the present study we determined the in vivo distribution of the venom following its subcutaneous injection into mice. B. erythromelas venom and albumin were labeled individually with 131I by the chloramine T method, and separated in a Sephacryl® S-200 column. The efficiency of labeling was 68%. Male Swiss mice (40-45 g), which had been provided with drinking water containing 0.05% KI over a period of 10 days prior to the experiment, were inoculated dorsally (sc) with 0.3 ml (2.35 x 105 cpm/mouse) of 131I-venom (N = 42), 131I-albumin or 131I (controls, N = 28 each). Thirty minutes and 1, 3, 6, 12, 18 and 24 h after inoculation, the animals were perfused with 0.85% NaCl and skin and various organs were collected in order to determine radioactivity content. There was a high rate of venom absorption in the skin (51%) within the first 30 min compared to albumin (20.1%) and free iodine (8.2%). Up to the third hour after injection there was a tendency for venom and albumin to concentrate in the stomach (3rd h), small intestine (3rd h) and large intestine (6th h). Both control groups had more radioactivity in the digestive tract, especially in the stomach, but these levels decreased essentially to baseline by 12-18 h postinjection. In the kidneys, the distribution profiles of venom, albumin and iodine were similar. Counts at 30 min postinjection were low in all three groups (1.37, 1.86 and 0.77, respectively), and diminished to essentially 0% by 12-18 h. Albumin tended to concentrate in muscle until the 3rd h postinjection (1.98%). There was a low binding of labeled venom in the liver (<0.54%), thyroid (<0.11%) and lungs (<0.08%), and no iodinated venom was detected in brain, heart, diaphragm, spleen or bladder. The low venom binding observed in most internal organs, comparable to that of albumin, suggests that B. erythromelas venom does not specifically target most internal organs. That is, the systemic effects of envenomation are mainly due to an indirect action.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2012-10-24T18:45:51Z No. of bitstreams: 1 Vasconcelos, C.M.L..pdf: 46049 bytes, checksum: d475f4c37c3e577b736f098005371a4b (MD5)Made available in DSpace on 2012-10-24T18:45:51Z (GMT). No. of bitstreams: 1 Vasconcelos, C.M.L..pdf: 46049 bytes, checksum: d475f4c37c3e577b736f098005371a4b (MD5) Previous issue date: 1998Ribeirão PretoBraz J Med Biol Reshttp://dx.doi.org/10.1590/S0100-879X1998000300017reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABothrops erythromelassnake venomprotein iodinationDistribution of 131I-labeled Bothrops erythromelas venom in miceBraz J Med Biol Resinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALVasconcelos, C.M.L..pdfVasconcelos, C.M.L..pdfapplication/pdf46049https://repositorio.ufba.br/bitstream/ri/7050/1/Vasconcelos%2c%20C.M.L..pdfd475f4c37c3e577b736f098005371a4bMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/7050/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXTVasconcelos, C.M.L..pdf.txtVasconcelos, C.M.L..pdf.txtExtracted texttext/plain18098https://repositorio.ufba.br/bitstream/ri/7050/3/Vasconcelos%2c%20C.M.L..pdf.txt0072ba9a63e8ae07a7867aabb01cdb52MD53ri/70502022-07-05 14:03:12.262oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:12Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Distribution of 131I-labeled Bothrops erythromelas venom in mice
dc.title.alternative.pt_BR.fl_str_mv Braz J Med Biol Res
title Distribution of 131I-labeled Bothrops erythromelas venom in mice
spellingShingle Distribution of 131I-labeled Bothrops erythromelas venom in mice
Vasconcelos, C. M. L.
Bothrops erythromelas
snake venom
protein iodination
title_short Distribution of 131I-labeled Bothrops erythromelas venom in mice
title_full Distribution of 131I-labeled Bothrops erythromelas venom in mice
title_fullStr Distribution of 131I-labeled Bothrops erythromelas venom in mice
title_full_unstemmed Distribution of 131I-labeled Bothrops erythromelas venom in mice
title_sort Distribution of 131I-labeled Bothrops erythromelas venom in mice
author Vasconcelos, C. M. L.
author_facet Vasconcelos, C. M. L.
Valença, R. C.
Araújo, E. A.
Modesto, J. C. A.
Pontes, M. M.
Brazil, Tania Kobler
Guarnieri, M. C.
author_role author
author2 Valença, R. C.
Araújo, E. A.
Modesto, J. C. A.
Pontes, M. M.
Brazil, Tania Kobler
Guarnieri, M. C.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vasconcelos, C. M. L.
Valença, R. C.
Araújo, E. A.
Modesto, J. C. A.
Pontes, M. M.
Brazil, Tania Kobler
Guarnieri, M. C.
Vasconcelos, C. M. L.
Valença, R. C.
Araújo, E. A.
Modesto, J. C. A.
Pontes, M. M.
Brazil, Tania Kobler
Guarnieri, M. C.
dc.subject.por.fl_str_mv Bothrops erythromelas
snake venom
protein iodination
topic Bothrops erythromelas
snake venom
protein iodination
description p. 439-443
publishDate 1998
dc.date.issued.fl_str_mv 1998
dc.date.accessioned.fl_str_mv 2012-10-24T18:45:51Z
dc.date.available.fl_str_mv 2012-10-24T18:45:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7050
dc.identifier.issn.none.fl_str_mv 1414-431X
dc.identifier.number.pt_BR.fl_str_mv v. 31, n. 3
identifier_str_mv 1414-431X
v. 31, n. 3
url http://www.repositorio.ufba.br/ri/handle/ri/7050
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Braz J Med Biol Res
publisher.none.fl_str_mv Braz J Med Biol Res
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0100-879X1998000300017
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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