Distribution of 131I-labeled Bothrops erythromelas venom in mice
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/7050 |
Resumo: | p. 439-443 |
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Vasconcelos, C. M. L.Valença, R. C.Araújo, E. A.Modesto, J. C. A.Pontes, M. M.Brazil, Tania KoblerGuarnieri, M. C.Vasconcelos, C. M. L.Valença, R. C.Araújo, E. A.Modesto, J. C. A.Pontes, M. M.Brazil, Tania KoblerGuarnieri, M. C.2012-10-24T18:45:51Z2012-10-24T18:45:51Z19981414-431Xhttp://www.repositorio.ufba.br/ri/handle/ri/7050v. 31, n. 3p. 439-443Bothrops erythromelas is responsible for many snake bites in northeastern Brazil. In the present study we determined the in vivo distribution of the venom following its subcutaneous injection into mice. B. erythromelas venom and albumin were labeled individually with 131I by the chloramine T method, and separated in a Sephacryl® S-200 column. The efficiency of labeling was 68%. Male Swiss mice (40-45 g), which had been provided with drinking water containing 0.05% KI over a period of 10 days prior to the experiment, were inoculated dorsally (sc) with 0.3 ml (2.35 x 105 cpm/mouse) of 131I-venom (N = 42), 131I-albumin or 131I (controls, N = 28 each). Thirty minutes and 1, 3, 6, 12, 18 and 24 h after inoculation, the animals were perfused with 0.85% NaCl and skin and various organs were collected in order to determine radioactivity content. There was a high rate of venom absorption in the skin (51%) within the first 30 min compared to albumin (20.1%) and free iodine (8.2%). Up to the third hour after injection there was a tendency for venom and albumin to concentrate in the stomach (3rd h), small intestine (3rd h) and large intestine (6th h). Both control groups had more radioactivity in the digestive tract, especially in the stomach, but these levels decreased essentially to baseline by 12-18 h postinjection. In the kidneys, the distribution profiles of venom, albumin and iodine were similar. Counts at 30 min postinjection were low in all three groups (1.37, 1.86 and 0.77, respectively), and diminished to essentially 0% by 12-18 h. Albumin tended to concentrate in muscle until the 3rd h postinjection (1.98%). There was a low binding of labeled venom in the liver (<0.54%), thyroid (<0.11%) and lungs (<0.08%), and no iodinated venom was detected in brain, heart, diaphragm, spleen or bladder. The low venom binding observed in most internal organs, comparable to that of albumin, suggests that B. erythromelas venom does not specifically target most internal organs. That is, the systemic effects of envenomation are mainly due to an indirect action.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2012-10-24T18:45:51Z No. of bitstreams: 1 Vasconcelos, C.M.L..pdf: 46049 bytes, checksum: d475f4c37c3e577b736f098005371a4b (MD5)Made available in DSpace on 2012-10-24T18:45:51Z (GMT). No. of bitstreams: 1 Vasconcelos, C.M.L..pdf: 46049 bytes, checksum: d475f4c37c3e577b736f098005371a4b (MD5) Previous issue date: 1998Ribeirão PretoBraz J Med Biol Reshttp://dx.doi.org/10.1590/S0100-879X1998000300017reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABothrops erythromelassnake venomprotein iodinationDistribution of 131I-labeled Bothrops erythromelas venom in miceBraz J Med Biol Resinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALVasconcelos, C.M.L..pdfVasconcelos, C.M.L..pdfapplication/pdf46049https://repositorio.ufba.br/bitstream/ri/7050/1/Vasconcelos%2c%20C.M.L..pdfd475f4c37c3e577b736f098005371a4bMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/7050/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXTVasconcelos, C.M.L..pdf.txtVasconcelos, C.M.L..pdf.txtExtracted texttext/plain18098https://repositorio.ufba.br/bitstream/ri/7050/3/Vasconcelos%2c%20C.M.L..pdf.txt0072ba9a63e8ae07a7867aabb01cdb52MD53ri/70502022-07-05 14:03:12.262oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:12Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Distribution of 131I-labeled Bothrops erythromelas venom in mice |
dc.title.alternative.pt_BR.fl_str_mv |
Braz J Med Biol Res |
title |
Distribution of 131I-labeled Bothrops erythromelas venom in mice |
spellingShingle |
Distribution of 131I-labeled Bothrops erythromelas venom in mice Vasconcelos, C. M. L. Bothrops erythromelas snake venom protein iodination |
title_short |
Distribution of 131I-labeled Bothrops erythromelas venom in mice |
title_full |
Distribution of 131I-labeled Bothrops erythromelas venom in mice |
title_fullStr |
Distribution of 131I-labeled Bothrops erythromelas venom in mice |
title_full_unstemmed |
Distribution of 131I-labeled Bothrops erythromelas venom in mice |
title_sort |
Distribution of 131I-labeled Bothrops erythromelas venom in mice |
author |
Vasconcelos, C. M. L. |
author_facet |
Vasconcelos, C. M. L. Valença, R. C. Araújo, E. A. Modesto, J. C. A. Pontes, M. M. Brazil, Tania Kobler Guarnieri, M. C. |
author_role |
author |
author2 |
Valença, R. C. Araújo, E. A. Modesto, J. C. A. Pontes, M. M. Brazil, Tania Kobler Guarnieri, M. C. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vasconcelos, C. M. L. Valença, R. C. Araújo, E. A. Modesto, J. C. A. Pontes, M. M. Brazil, Tania Kobler Guarnieri, M. C. Vasconcelos, C. M. L. Valença, R. C. Araújo, E. A. Modesto, J. C. A. Pontes, M. M. Brazil, Tania Kobler Guarnieri, M. C. |
dc.subject.por.fl_str_mv |
Bothrops erythromelas snake venom protein iodination |
topic |
Bothrops erythromelas snake venom protein iodination |
description |
p. 439-443 |
publishDate |
1998 |
dc.date.issued.fl_str_mv |
1998 |
dc.date.accessioned.fl_str_mv |
2012-10-24T18:45:51Z |
dc.date.available.fl_str_mv |
2012-10-24T18:45:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/7050 |
dc.identifier.issn.none.fl_str_mv |
1414-431X |
dc.identifier.number.pt_BR.fl_str_mv |
v. 31, n. 3 |
identifier_str_mv |
1414-431X v. 31, n. 3 |
url |
http://www.repositorio.ufba.br/ri/handle/ri/7050 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Braz J Med Biol Res |
publisher.none.fl_str_mv |
Braz J Med Biol Res |
dc.source.pt_BR.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X1998000300017 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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Universidade Federal da Bahia (UFBA) |
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UFBA |
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UFBA |
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Repositório Institucional da UFBA |
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Repositório Institucional da UFBA |
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