An improved anti-C3/IgG ELISA for quantification of soluble immune complexes

Detalhes bibliográficos
Autor(a) principal: Soares, Neci Matos
Data de Publicação: 2001
Outros Autores: Santiago, Mittermayer Barreto, Carvalho, Lain Carlos Pontes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7764
Resumo: Texto completo: acesso restrito. p. 199–205
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spelling Soares, Neci MatosSantiago, Mittermayer BarretoCarvalho, Lain Carlos Pontes deSoares, Neci MatosSantiago, Mittermayer BarretoCarvalho, Lain Carlos Pontes de2013-01-04T15:12:34Z20010022-1759http://www.repositorio.ufba.br/ri/handle/ri/7764v. 249, n. 1-2Texto completo: acesso restrito. p. 199–205A semi-quantitative ELISA for complement-fixing, IgG-containing immune complexes (IC) is described. The assay is based on the insolubilization of IC by polyethyleneglycol, their capture by solid-phase anti-C3 antibodies, reaction with peroxidase-labeled anti-IgG antibodies and incubation with a chromogenic peroxidase substrate. It was markedly improved by the use of a single-step procedure which simultaneously washed and precipitated the insolubilized immune complexes. Intra-assay and inter-assay coefficients of variation were lower than 8.6 and 14.7%, respectively. As expected, higher levels of circulating immune complexes, in relation to healthy individuals, were found in patients with American visceral leishmaniasis (AVL), systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), with prevalences comparable to those described in the literature. The ELISA can be quickly assembled from reagents and plasticware widely available commercially, detects immune complexes fulfilling three different criteria and is more sensitive than a previously published method based on the same principles (detection limit for complement-sensitized aggregated IgG of 2 μg ml−1 as compared with a detection limit above 16 μg ml−1).Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2013-01-04T15:12:34Z No. of bitstreams: 1 Soares.pdf: 191788 bytes, checksum: b78c44651d8f3a884fc8fc69cbb05f66 (MD5)Made available in DSpace on 2013-01-04T15:12:34Z (GMT). No. of bitstreams: 1 Soares.pdf: 191788 bytes, checksum: b78c44651d8f3a884fc8fc69cbb05f66 (MD5) Previous issue date: 2001http://dx.doi.org/10.1016/S0022-1759(00)00362-8reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAImmune complexesPolyethylene glycolELISAC3IgGAn improved anti-C3/IgG ELISA for quantification of soluble immune complexesJournal of Immunological Methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINALSoares.pdfSoares.pdfapplication/pdf191788https://repositorio.ufba.br/bitstream/ri/7764/1/Soares.pdfb78c44651d8f3a884fc8fc69cbb05f66MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7764/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTSoares.pdf.txtSoares.pdf.txtExtracted texttext/plain24653https://repositorio.ufba.br/bitstream/ri/7764/3/Soares.pdf.txt25a264082f49dce2f9b3b45db1dab2aeMD53ri/77642022-07-05 14:03:07.722oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:07Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
dc.title.alternative.pt_BR.fl_str_mv Journal of Immunological Methods
title An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
spellingShingle An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
Soares, Neci Matos
Immune complexes
Polyethylene glycol
ELISA
C3
IgG
title_short An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_full An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_fullStr An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_full_unstemmed An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
title_sort An improved anti-C3/IgG ELISA for quantification of soluble immune complexes
author Soares, Neci Matos
author_facet Soares, Neci Matos
Santiago, Mittermayer Barreto
Carvalho, Lain Carlos Pontes de
author_role author
author2 Santiago, Mittermayer Barreto
Carvalho, Lain Carlos Pontes de
author2_role author
author
dc.contributor.author.fl_str_mv Soares, Neci Matos
Santiago, Mittermayer Barreto
Carvalho, Lain Carlos Pontes de
Soares, Neci Matos
Santiago, Mittermayer Barreto
Carvalho, Lain Carlos Pontes de
dc.subject.por.fl_str_mv Immune complexes
Polyethylene glycol
ELISA
C3
IgG
topic Immune complexes
Polyethylene glycol
ELISA
C3
IgG
description Texto completo: acesso restrito. p. 199–205
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2013-01-04T15:12:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7764
dc.identifier.issn.none.fl_str_mv 0022-1759
dc.identifier.number.pt_BR.fl_str_mv v. 249, n. 1-2
identifier_str_mv 0022-1759
v. 249, n. 1-2
url http://www.repositorio.ufba.br/ri/handle/ri/7764
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/S0022-1759(00)00362-8
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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