Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea

Detalhes bibliográficos
Autor(a) principal: Nascimento, Iracema Andrade
Data de Publicação: 1996
Outros Autores: Dickson, Kenneth L., Zimmerman, Earl G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/17084
Resumo: Texto completo: acesso restrito. p.231-238
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spelling Nascimento, Iracema AndradeDickson, Kenneth L.Zimmerman, Earl G.Nascimento, Iracema AndradeDickson, Kenneth L.Zimmerman, Earl G.2015-03-03T14:18:53Z19961573-5141http://repositorio.ufba.br/ri/handle/ri/17084v. 5, n. 4Texto completo: acesso restrito. p.231-238A recent approach to evaluate environmenta induced damages has been damages has been suggested, based on the stress response. The approach involves the detection of stress protein induction in organisms to infer about environmental conditions in their surroundings. However, to be an indicator of adverse biological effects in the environment, the elevation of stress proteins should be compared to a response pattern for the experimental species. JuvenileCorbicula fluminea, collection from a control site, were submited to heat-shock stress in the laboratory to obtain the stress response pattern under normal and extreme conditions. Acclimated to 26°C, the specimens were submited to 29, 32, 35 and 38°C, for 96 h. After 1, 2, 4, 8, 24, 48, 72 and 96 h of exposure, clams were removed from each vial and prepared for stress protein analysis. Animals from the control site were frozen in liquid nitrogen at the time of collection, and prepared for stress protein analysis. Hsp60 and 70 were detected by immunoreactivity after separation on 12.5% polyacrylamide gels and transference to nitrocellulose by western blotting, to determine the stress protein concentrations. The result showed that hsp70 increased at 4h from the beginning of the experiment and progressed over the 96 h experimental period in animals exposed to 35°C. However hsp70 levels decreased between 4 h and 24 h for the clams stressed at their lethal temperature of 38°C. Immunoblotting with hsp60 showed similar reactivity. At 38°C there was an increase in the amount of hsp60 at 4h, reaching a maximum eight-fold level at 8h. By 96h, the amount decreased to levels lower than those observed at 4h. At 38°C the level of hsp60 began to decrease at 8 h and continue to decline to 24 h when the clams died. The data support the hypothesis of increasing concentrations of stress protein until the heat shock approaches the thermal limits for the species. The results of this research suggest the usefulness of using the stress response as a diagnostic in environmental toxicology. They confirm that the sps response may serve as a valid biomonitoring tool under chronic, sublethal exposures when it is still possible to prevent effects at organismal or higher organizational levels.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2015-03-03T13:23:10Z No. of bitstreams: 1 33-J. Aqua. Ecosys. Health 5 p. 231-238, 1996.pdf: 4045398 bytes, checksum: 5fb2dc7602b5e16bd8d22f9758a87260 (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2015-03-03T14:18:53Z (GMT) No. of bitstreams: 1 33-J. Aqua. Ecosys. Health 5 p. 231-238, 1996.pdf: 4045398 bytes, checksum: 5fb2dc7602b5e16bd8d22f9758a87260 (MD5)Made available in DSpace on 2015-03-03T14:18:53Z (GMT). No. of bitstreams: 1 33-J. Aqua. Ecosys. Health 5 p. 231-238, 1996.pdf: 4045398 bytes, checksum: 5fb2dc7602b5e16bd8d22f9758a87260 (MD5) Previous issue date: 1996http://dx.doi.org/10.1007/BF00662184reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAStress proteinsCorbicula flumineaStress responseHeat shock protein response to thermal stress in the Asiatic clam,Corbicula flumineaJournal of Aquatic Ecosystem Healthinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL33-J. Aqua. Ecosys. Health 5 p. 231-238, 1996.pdf33-J. Aqua. Ecosys. 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dc.title.pt_BR.fl_str_mv Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
dc.title.alternative.pt_BR.fl_str_mv Journal of Aquatic Ecosystem Health
title Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
spellingShingle Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
Nascimento, Iracema Andrade
Stress proteins
Corbicula fluminea
Stress response
title_short Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
title_full Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
title_fullStr Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
title_full_unstemmed Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
title_sort Heat shock protein response to thermal stress in the Asiatic clam,Corbicula fluminea
author Nascimento, Iracema Andrade
author_facet Nascimento, Iracema Andrade
Dickson, Kenneth L.
Zimmerman, Earl G.
author_role author
author2 Dickson, Kenneth L.
Zimmerman, Earl G.
author2_role author
author
dc.contributor.author.fl_str_mv Nascimento, Iracema Andrade
Dickson, Kenneth L.
Zimmerman, Earl G.
Nascimento, Iracema Andrade
Dickson, Kenneth L.
Zimmerman, Earl G.
dc.subject.por.fl_str_mv Stress proteins
Corbicula fluminea
Stress response
topic Stress proteins
Corbicula fluminea
Stress response
description Texto completo: acesso restrito. p.231-238
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2015-03-03T14:18:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/17084
dc.identifier.issn.none.fl_str_mv 1573-5141
dc.identifier.number.pt_BR.fl_str_mv v. 5, n. 4
identifier_str_mv 1573-5141
v. 5, n. 4
url http://repositorio.ufba.br/ri/handle/ri/17084
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1007/BF00662184
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
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