Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/12858 |
Resumo: | Texto completo: acesso restrito. p.199-212 |
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Oliveira, Diêgo Madureira dePitanga, B. P. S.Grangeiro, Maria SocorroLima, R. M. F.Costa, Maria de Fátima DiasCosta, Silvia LimaClarêncio, J.El-Bachá, Ramon dos SantosOliveira, Diêgo Madureira dePitanga, B. P. S.Grangeiro, Maria SocorroLima, R. M. F.Costa, Maria de Fátima DiasCosta, Silvia LimaClarêncio, J.El-Bachá, Ramon dos Santos2013-09-06T15:24:44Z20100960-3271http://www.repositorio.ufba.br/ri/handle/ri/12858v. 29, n. 3Texto completo: acesso restrito. p.199-212The exposure to benzene is a public health problem. Although the most well-known effect of benzene is hematopoietic toxicity, there is little information about the benzene and its metabolites effects on the central nervous system (CNS). This study examined the toxic effects of 1,2-dihydroxybenzene (catechol), a benzene metabolite, to human glioblastoma GL-15 cells. GL-15 cell cultures were used as a model to provide more information about the toxic effects of aromatic compounds to the CNS. Catechol induced time- and concentration-dependent cytotoxic effects. Morphological changes, such as the retraction of the cytoplasm and chromatin clumping, were seen in cells exposed to 200 μM catechol for 48 hours. In cells exposed to 600 μM catechol for 48 hours, 78.0% of them presented condensed nuclei, and the Comet assay showed DNA damage. The percentage of cells labeled with annexin V (apoptotic cells) was greater in the group exposed to catechol (20.7%) than in control cells (0.4%). Exposure to catechol at concentrations greater than 100 μM enhanced Bax levels, and a decrease in Bcl-2 level was observed after the exposure to 600 μM catechol for 48 hours. Furthermore, catechol depleted reduced glutathione. Hence, catechol induced cell death mainly by apoptosis.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-09-05T12:45:29Z No. of bitstreams: 1 Hum Exp Toxicol-2010-de Oliveira-199-212.pdf: 859208 bytes, checksum: bf3926c1528ac250894eed99bcac8cda (MD5)Approved for entry into archive by Patricia Barroso(pbarroso@ufba.br) on 2013-09-06T15:24:44Z (GMT) No. of bitstreams: 1 Hum Exp Toxicol-2010-de Oliveira-199-212.pdf: 859208 bytes, checksum: bf3926c1528ac250894eed99bcac8cda (MD5)Made available in DSpace on 2013-09-06T15:24:44Z (GMT). No. of bitstreams: 1 Hum Exp Toxicol-2010-de Oliveira-199-212.pdf: 859208 bytes, checksum: bf3926c1528ac250894eed99bcac8cda (MD5) Previous issue date: 2010http://dx.doi.org/10.1177/0960327109360364reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAApoptosisBaxBcl-2CatecholCytotoxicityGlioblastomaCatechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosisHuman and Experimental Toxicologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINALHum Exp Toxicol-2010-de Oliveira-199-212.pdfHum Exp Toxicol-2010-de Oliveira-199-212.pdfapplication/pdf859208https://repositorio.ufba.br/bitstream/ri/12858/1/Hum%20Exp%20Toxicol-2010-de%20Oliveira-199-212.pdfbf3926c1528ac250894eed99bcac8cdaMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12858/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTHum Exp Toxicol-2010-de Oliveira-199-212.pdf.txtHum Exp Toxicol-2010-de Oliveira-199-212.pdf.txtExtracted texttext/plain48365https://repositorio.ufba.br/bitstream/ri/12858/3/Hum%20Exp%20Toxicol-2010-de%20Oliveira-199-212.pdf.txt30cb06ff7fff9b26d872e73d569c235bMD53ri/128582022-07-05 14:03:42.569oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:42Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis |
| dc.title.alternative.pt_BR.fl_str_mv |
Human and Experimental Toxicology |
| title |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis |
| spellingShingle |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis Oliveira, Diêgo Madureira de Apoptosis Bax Bcl-2 Catechol Cytotoxicity Glioblastoma |
| title_short |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis |
| title_full |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis |
| title_fullStr |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis |
| title_full_unstemmed |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis |
| title_sort |
Catechol cytotoxicity in vitro: Induction of glioblastoma cell death by apoptosis |
| author |
Oliveira, Diêgo Madureira de |
| author_facet |
Oliveira, Diêgo Madureira de Pitanga, B. P. S. Grangeiro, Maria Socorro Lima, R. M. F. Costa, Maria de Fátima Dias Costa, Silvia Lima Clarêncio, J. El-Bachá, Ramon dos Santos |
| author_role |
author |
| author2 |
Pitanga, B. P. S. Grangeiro, Maria Socorro Lima, R. M. F. Costa, Maria de Fátima Dias Costa, Silvia Lima Clarêncio, J. El-Bachá, Ramon dos Santos |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Diêgo Madureira de Pitanga, B. P. S. Grangeiro, Maria Socorro Lima, R. M. F. Costa, Maria de Fátima Dias Costa, Silvia Lima Clarêncio, J. El-Bachá, Ramon dos Santos Oliveira, Diêgo Madureira de Pitanga, B. P. S. Grangeiro, Maria Socorro Lima, R. M. F. Costa, Maria de Fátima Dias Costa, Silvia Lima Clarêncio, J. El-Bachá, Ramon dos Santos |
| dc.subject.por.fl_str_mv |
Apoptosis Bax Bcl-2 Catechol Cytotoxicity Glioblastoma |
| topic |
Apoptosis Bax Bcl-2 Catechol Cytotoxicity Glioblastoma |
| description |
Texto completo: acesso restrito. p.199-212 |
| publishDate |
2010 |
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2010 |
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2013-09-06T15:24:44Z |
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0960-3271 |
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v. 29, n. 3 |
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0960-3271 v. 29, n. 3 |
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