Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis

Detalhes bibliográficos
Autor(a) principal: Paule, B. J. A.
Data de Publicação: 2004
Outros Autores: Nascimento, Roberto José Meyer, Costa, L. F. Moura, Bahia, Robson Cerqueira, Carminati, R., Regis, Lia Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7696
Resumo: Texto completo:acesso restrito. p. 311-316
id UFBA-2_e2d2ddf600f8772582fe84e90dedf257
oai_identifier_str oai:repositorio.ufba.br:ri/7696
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Paule, B. J. A.Nascimento, Roberto José MeyerCosta, L. F. MouraBahia, Robson CerqueiraCarminati, R.Regis, Lia FernandesPaule, B. J. A.Nascimento, Roberto José MeyerCosta, L. F. MouraBahia, Robson CerqueiraCarminati, R.Regis, Lia Fernandes2012-12-20T17:38:35Z20041046-5928http://www.repositorio.ufba.br/ri/handle/ri/7696v. 34, n. 2Texto completo:acesso restrito. p. 311-316The three-phase partitioning (TPP) technique was used upstream to isolate/concentrate secreted proteins from Corynebacterium pseudotuberculosis cultured in a complex liquid medium. Several parameters of the TPP technique (15, 30, or 60% ammonium sulfate concentration; 4.0, 5.5, or 7.0 pH; and primary (n) or tertiary (t)-butanol solvent isomer) were varied to determine the optimal recovery of serologically and cellularly immunoreactive extracted proteins. A TPP extraction made with 30% ammonium sulfate and an initial pH of 4.0 gave the best humoral and cellular immunoreactivity of caseous lymphadenitis infected goats. In particular, two immunogenic secreted (16 and 125 kDa) proteins, which had not been found by other extraction methods, were identified.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-12-20T17:38:35Z No. of bitstreams: 1 1-s2.0-S1046592804000051-main.pdf: 511792 bytes, checksum: af3c9b021fa8564642b5c578dee51d18 (MD5)Made available in DSpace on 2012-12-20T17:38:35Z (GMT). No. of bitstreams: 1 1-s2.0-S1046592804000051-main.pdf: 511792 bytes, checksum: af3c9b021fa8564642b5c578dee51d18 (MD5) Previous issue date: 2004Elsevierhttp://dx.doi.org/10.1016/j.pep.2003.12.003reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACorynebacterium pseudotuberculosisExcreted–secreted proteinsThree-phase partitioningThree-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosisProtein Expression and Purificationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S1046592804000051-main.pdf1-s2.0-S1046592804000051-main.pdfapplication/pdf511792https://repositorio.ufba.br/bitstream/ri/7696/1/1-s2.0-S1046592804000051-main.pdfaf3c9b021fa8564642b5c578dee51d18MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7696/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S1046592804000051-main.pdf.txt1-s2.0-S1046592804000051-main.pdf.txtExtracted texttext/plain19886https://repositorio.ufba.br/bitstream/ri/7696/3/1-s2.0-S1046592804000051-main.pdf.txt60faf033ddf824935ce9592895cc10b7MD53ri/76962022-07-05 14:02:55.141oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:55Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
dc.title.alternative.pt_BR.fl_str_mv Protein Expression and Purification
title Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
spellingShingle Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
Paule, B. J. A.
Corynebacterium pseudotuberculosis
Excreted–secreted proteins
Three-phase partitioning
title_short Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
title_full Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
title_fullStr Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
title_full_unstemmed Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
title_sort Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis
author Paule, B. J. A.
author_facet Paule, B. J. A.
Nascimento, Roberto José Meyer
Costa, L. F. Moura
Bahia, Robson Cerqueira
Carminati, R.
Regis, Lia Fernandes
author_role author
author2 Nascimento, Roberto José Meyer
Costa, L. F. Moura
Bahia, Robson Cerqueira
Carminati, R.
Regis, Lia Fernandes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Paule, B. J. A.
Nascimento, Roberto José Meyer
Costa, L. F. Moura
Bahia, Robson Cerqueira
Carminati, R.
Regis, Lia Fernandes
Paule, B. J. A.
Nascimento, Roberto José Meyer
Costa, L. F. Moura
Bahia, Robson Cerqueira
Carminati, R.
Regis, Lia Fernandes
dc.subject.por.fl_str_mv Corynebacterium pseudotuberculosis
Excreted–secreted proteins
Three-phase partitioning
topic Corynebacterium pseudotuberculosis
Excreted–secreted proteins
Three-phase partitioning
description Texto completo:acesso restrito. p. 311-316
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2012-12-20T17:38:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7696
dc.identifier.issn.none.fl_str_mv 1046-5928
dc.identifier.number.pt_BR.fl_str_mv v. 34, n. 2
identifier_str_mv 1046-5928
v. 34, n. 2
url http://www.repositorio.ufba.br/ri/handle/ri/7696
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.pep.2003.12.003
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/7696/1/1-s2.0-S1046592804000051-main.pdf
https://repositorio.ufba.br/bitstream/ri/7696/2/license.txt
https://repositorio.ufba.br/bitstream/ri/7696/3/1-s2.0-S1046592804000051-main.pdf.txt
bitstream.checksum.fl_str_mv af3c9b021fa8564642b5c578dee51d18
1b89a9a0548218172d7c829f87a0eab9
60faf033ddf824935ce9592895cc10b7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459414142713856

Registros relacionados