Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/15737 |
Resumo: | Texto completo: acesso restrito. p. 301–310 |
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Rossi, Andre L.Barreto, Isabela CerqueiraMaciel, William Q.Rosa, Fabiana PaimLeão, Maria H. RochaWerckmann, JacquesRossi, Alexandre MaltaBorojevic, RadovanFarina, MarcosRossi, Andre L.Barreto, Isabela CerqueiraMaciel, William Q.Rosa, Fabiana PaimLeão, Maria H. RochaWerckmann, JacquesRossi, Alexandre MaltaBorojevic, RadovanFarina, Marcos2014-08-28T21:31:27Z20128756-3282http://repositorio.ufba.br/ri/handle/ri/15737v. 50, n. 1Texto completo: acesso restrito. p. 301–310We report the ultrastructure of regenerated bone surrounding two types of biomaterials: hydroxyapatite–alginate composite and sintered hydroxyapatite. Critical defects in the calvaria of Wistar rats were filled with micrometer-sized spherical biomaterials and analyzed after 90 and 120 days of implantation by high-resolution transmission electron microscopy and Fourier transform infrared attenuated total reflectance microscopy, respectively. Infrared spectroscopy showed that hydroxyapatite of both biomaterials became more disordered after implantation in the rat calvaria, indicating that the biological environment induced modifications in biomaterials structure. We observed that the regenerated bone surrounding both biomaterials had a lamellar structure with type I collagen fibers alternating in adjacent lamella with angles of approximately 90°. In each lamella, plate-like apatite crystals were aligned in the c-axis direction, although a rotation around the c-axis could be present. Bone plate-like crystal dimensions were similar in regenerated bone around biomaterials and pre-existing bone in the rat calvaria. No epitaxial growth was observed around any of the biomaterials. A distinct mineralized layer was observed between new bone and hydroxyapatite–alginate biomaterial. This region presented a particular ultrastructure with crystallites smaller than those of the bulk of the biomaterial, and was possibly formed during the synthesis of alginate-containing composite or in the biological environment after implantation. Round nanoparticles were observed in regions of newly formed bone. The findings of this work contribute to a better understanding of the role of hydroxyapatite based biomaterials in bone regeneration processes at the nanoscale.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-27T15:11:22Z No. of bitstreams: 1 Andre L. Rossi.pdf: 2285072 bytes, checksum: f0610b4fbca7b37b207d359d1dab3c95 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-08-28T21:31:27Z (GMT) No. of bitstreams: 1 Andre L. Rossi.pdf: 2285072 bytes, checksum: f0610b4fbca7b37b207d359d1dab3c95 (MD5)Made available in DSpace on 2014-08-28T21:31:27Z (GMT). No. of bitstreams: 1 Andre L. Rossi.pdf: 2285072 bytes, checksum: f0610b4fbca7b37b207d359d1dab3c95 (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1016/j.bone.2011.10.022reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABone implantHydroxyapatite–alginate compositeBone ultrastructureBiomaterialsBiomineralizationHRTEMUltrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatiteBoneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALAndre L. Rossi.pdfAndre L. Rossi.pdfapplication/pdf2285072https://repositorio.ufba.br/bitstream/ri/15737/1/Andre%20L.%20Rossi.pdff0610b4fbca7b37b207d359d1dab3c95MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15737/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTAndre L. Rossi.pdf.txtAndre L. Rossi.pdf.txtExtracted texttext/plain55170https://repositorio.ufba.br/bitstream/ri/15737/3/Andre%20L.%20Rossi.pdf.txt915a9f77a422ed7464feb238e4840a9dMD53ri/157372022-07-05 14:03:33.388oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:33Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite |
| dc.title.alternative.pt_BR.fl_str_mv |
Bone |
| title |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite |
| spellingShingle |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite Rossi, Andre L. Bone implant Hydroxyapatite–alginate composite Bone ultrastructure Biomaterials Biomineralization HRTEM |
| title_short |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite |
| title_full |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite |
| title_fullStr |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite |
| title_full_unstemmed |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite |
| title_sort |
Ultrastructure of regenerated bone mineral surrounding hydroxyapatite–alginate composite and sintered hydroxyapatite |
| author |
Rossi, Andre L. |
| author_facet |
Rossi, Andre L. Barreto, Isabela Cerqueira Maciel, William Q. Rosa, Fabiana Paim Leão, Maria H. Rocha Werckmann, Jacques Rossi, Alexandre Malta Borojevic, Radovan Farina, Marcos |
| author_role |
author |
| author2 |
Barreto, Isabela Cerqueira Maciel, William Q. Rosa, Fabiana Paim Leão, Maria H. Rocha Werckmann, Jacques Rossi, Alexandre Malta Borojevic, Radovan Farina, Marcos |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rossi, Andre L. Barreto, Isabela Cerqueira Maciel, William Q. Rosa, Fabiana Paim Leão, Maria H. Rocha Werckmann, Jacques Rossi, Alexandre Malta Borojevic, Radovan Farina, Marcos Rossi, Andre L. Barreto, Isabela Cerqueira Maciel, William Q. Rosa, Fabiana Paim Leão, Maria H. Rocha Werckmann, Jacques Rossi, Alexandre Malta Borojevic, Radovan Farina, Marcos |
| dc.subject.por.fl_str_mv |
Bone implant Hydroxyapatite–alginate composite Bone ultrastructure Biomaterials Biomineralization HRTEM |
| topic |
Bone implant Hydroxyapatite–alginate composite Bone ultrastructure Biomaterials Biomineralization HRTEM |
| description |
Texto completo: acesso restrito. p. 301–310 |
| publishDate |
2012 |
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2012 |
| dc.date.accessioned.fl_str_mv |
2014-08-28T21:31:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://repositorio.ufba.br/ri/handle/ri/15737 |
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8756-3282 |
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v. 50, n. 1 |
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8756-3282 v. 50, n. 1 |
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http://repositorio.ufba.br/ri/handle/ri/15737 |
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eng |
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info:eu-repo/semantics/openAccess |
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http://dx.doi.org/ 10.1016/j.bone.2011.10.022 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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