A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5531 |
Resumo: | p. 14 p. |
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Pacheco, Luis Gustavo CarvalhoSlade, Susan E.Seyffert, NúbiaSantos, Anderson Rodrigues dosCastro, Thiago Luiz de PaulaSilva, Wanderson M.Santos, Agenor V.Santos, Simone Gonçalves dosFarias, Luiz de MacêdoCarvalho, Maria Auxiliadora Roque dePimenta, Adriano Monteiro de CastroNascimento, Roberto José MeyerSilva, Artur Luiz da Costa daScrivens, James H.Oliveira, Sérgio CostaMiyoshi, AndersonDownson, Christopher G.Azevedo, Vasco Ariston de CarvalhoPacheco, Luis Gustavo CarvalhoSlade, Susan E.Seyffert, NúbiaSantos, Anderson Rodrigues dosCastro, Thiago Luiz de PaulaSilva, Wanderson M.Santos, Agenor V.Santos, Simone Gonçalves dosFarias, Luiz de MacêdoCarvalho, Maria Auxiliadora Roque dePimenta, Adriano Monteiro de CastroNascimento, Roberto José MeyerSilva, Artur Luiz da Costa daScrivens, James H.Oliveira, Sérgio CostaMiyoshi, AndersonDownson, Christopher G.Azevedo, Vasco Ariston de Carvalho2012-03-12T17:57:53Z2012-03-12T17:57:53Z20111471-2180http://www.repositorio.ufba.br/ri/handle/ri/553111:12p. 14 p.Background: Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing the entire exoproteome of the pathogenic bacterium Corynebacterium pseudotuberculosis, the etiological agent of caseous lymphadenitis (CLA) in sheep and goats. Results: An optimized protocol of three-phase partitioning (TPP) was used to obtain the C. pseudotuberculosis exoproteins, and a newly introduced method of data-independent MS acquisition (LC-MSE) was employed for protein identification and label-free quantification. Additionally, the recently developed tool SurfG+ was used for in silico prediction of sub-cellular localization of the identified proteins. In total, 93 different extracellular proteins of C. pseudotuberculosis were identified with high confidence by this strategy; 44 proteins were commonly identified in two different strains, isolated from distinct hosts, then composing a core C. pseudotuberculosis exoproteome. Analysis with the SurfG+ tool showed that more than 75% (70/93) of the identified proteins could be predicted as containing signals for active exportation. Moreover, evidence could be found for probable non-classical export of most of the remaining proteins. Conclusions: Comparative analyses of the exoproteomes of two C. pseudotuberculosis strains, in addition to comparison with other experimentally determined corynebacterial exoproteomes, were helpful to gain novel insights into the contribution of the exported proteins in the virulence of this bacterium. The results presented here compose the most comprehensive coverage of the exoproteome of a corynebacterial species so far.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-03-12T17:57:53Z No. of bitstreams: 1 A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.pdf: 502566 bytes, checksum: 1391b29b1a2008f44cf5ea68e325d340 (MD5)Made available in DSpace on 2012-03-12T17:57:53Z (GMT). No. of bitstreams: 1 A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.pdf: 502566 bytes, checksum: 1391b29b1a2008f44cf5ea68e325d340 (MD5) Previous issue date: 2011A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosisBMC Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5531/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINALA combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.pdfA combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.pdfapplication/pdf502566https://repositorio.ufba.br/bitstream/ri/5531/1/A%20combined%20approach%20for%20comparative%20exoproteome%20analysis%20of%20Corynebacterium%20pseudotuberculosis.pdf1391b29b1a2008f44cf5ea68e325d340MD51TEXTA combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.pdf.txtA combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis.pdf.txtExtracted texttext/plain76277https://repositorio.ufba.br/bitstream/ri/5531/3/A%20combined%20approach%20for%20comparative%20exoproteome%20analysis%20of%20Corynebacterium%20pseudotuberculosis.pdf.txt78228da2ad9daa4c5fb888db592ca107MD53ri/55312022-07-05 14:02:50.07oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:50Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis |
| dc.title.alternative.pt_BR.fl_str_mv |
BMC Microbiology |
| title |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis |
| spellingShingle |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis Pacheco, Luis Gustavo Carvalho |
| title_short |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis |
| title_full |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis |
| title_fullStr |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis |
| title_full_unstemmed |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis |
| title_sort |
A combined approach for comparative exoproteome analysis of Corynebacterium pseudotuberculosis |
| author |
Pacheco, Luis Gustavo Carvalho |
| author_facet |
Pacheco, Luis Gustavo Carvalho Slade, Susan E. Seyffert, Núbia Santos, Anderson Rodrigues dos Castro, Thiago Luiz de Paula Silva, Wanderson M. Santos, Agenor V. Santos, Simone Gonçalves dos Farias, Luiz de Macêdo Carvalho, Maria Auxiliadora Roque de Pimenta, Adriano Monteiro de Castro Nascimento, Roberto José Meyer Silva, Artur Luiz da Costa da Scrivens, James H. Oliveira, Sérgio Costa Miyoshi, Anderson Downson, Christopher G. Azevedo, Vasco Ariston de Carvalho |
| author_role |
author |
| author2 |
Slade, Susan E. Seyffert, Núbia Santos, Anderson Rodrigues dos Castro, Thiago Luiz de Paula Silva, Wanderson M. Santos, Agenor V. Santos, Simone Gonçalves dos Farias, Luiz de Macêdo Carvalho, Maria Auxiliadora Roque de Pimenta, Adriano Monteiro de Castro Nascimento, Roberto José Meyer Silva, Artur Luiz da Costa da Scrivens, James H. Oliveira, Sérgio Costa Miyoshi, Anderson Downson, Christopher G. Azevedo, Vasco Ariston de Carvalho |
| author2_role |
author author author author author author author author author author author author author author author author author |
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Pacheco, Luis Gustavo Carvalho Slade, Susan E. Seyffert, Núbia Santos, Anderson Rodrigues dos Castro, Thiago Luiz de Paula Silva, Wanderson M. Santos, Agenor V. Santos, Simone Gonçalves dos Farias, Luiz de Macêdo Carvalho, Maria Auxiliadora Roque de Pimenta, Adriano Monteiro de Castro Nascimento, Roberto José Meyer Silva, Artur Luiz da Costa da Scrivens, James H. Oliveira, Sérgio Costa Miyoshi, Anderson Downson, Christopher G. Azevedo, Vasco Ariston de Carvalho Pacheco, Luis Gustavo Carvalho Slade, Susan E. Seyffert, Núbia Santos, Anderson Rodrigues dos Castro, Thiago Luiz de Paula Silva, Wanderson M. Santos, Agenor V. Santos, Simone Gonçalves dos Farias, Luiz de Macêdo Carvalho, Maria Auxiliadora Roque de Pimenta, Adriano Monteiro de Castro Nascimento, Roberto José Meyer Silva, Artur Luiz da Costa da Scrivens, James H. Oliveira, Sérgio Costa Miyoshi, Anderson Downson, Christopher G. Azevedo, Vasco Ariston de Carvalho |
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p. 14 p. |
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2011 |
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2011 |
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2012-03-12T17:57:53Z |
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1471-2180 |
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