The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection

Detalhes bibliográficos
Autor(a) principal: Inês, Elizabete de Jesus
Data de Publicação: 2013
Outros Autores: Silva, Mônica Lopes Sampaio, Souza, Joelma Nascimento, Teixeira, Márcia Cristina Aquino, Soares, Neci Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/11969
Resumo: Texto completo. Acesso restrito. p. 31-35
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spelling Inês, Elizabete de JesusSilva, Mônica Lopes SampaioSouza, Joelma NascimentoTeixeira, Márcia Cristina AquinoSoares, Neci MatosInês, Elizabete de JesusSilva, Mônica Lopes SampaioSouza, Joelma NascimentoTeixeira, Márcia Cristina AquinoSoares, Neci Matos2013-06-18T20:41:48Z2013-06-18T20:41:48Z20130732-8893http://www.repositorio.ufba.br/ri/handle/ri/11969v. 76, n. 1Texto completo. Acesso restrito. p. 31-35Carbohydrates of pathogen antigens have been disrupted by periodate oxidation, in order to reduce nonspecific bindings and improve serodiagnosis of parasite infections. In the present study, the enzymelinked immunosorbent assay (ELISA) was carried out with filariform larvae antigen treated, or not treated, with sodium metaperiodate. Groups of sera from patients with Strongyloides stercoralis infection, with other intestinal parasites and a normal control, were used. The oxidation of Strongyloides stercoralis glycosylated epitopes reduced the seroreactivity of sera from patients with S. stercoralis infection as demonstrated by ELISA, with a decrease in sera optical densities. The number of cross-reactions of IgG and IgE-ELISAs increased by 12% and 16%, respectively, after antigen treatment with metaperiodate. This was more often observed in patients infected with Schistosoma mansoni and hookworm. Moreover, the IgG depletion from sera tested by IgE-ELISA led to the detection of previous false-negative samples from S. stercoralis–infected patients.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-06-12T18:06:42Z No. of bitstreams: 1 33333333.pdf: 306976 bytes, checksum: f89749f6edfe0cf5ba3776a5d131d1b2 (MD5)Approved for entry into archive by Alda Lima da Silva(sivalda@ufba.br) on 2013-06-18T20:41:48Z (GMT) No. of bitstreams: 1 33333333.pdf: 306976 bytes, checksum: f89749f6edfe0cf5ba3776a5d131d1b2 (MD5)Made available in DSpace on 2013-06-18T20:41:48Z (GMT). No. of bitstreams: 1 33333333.pdf: 306976 bytes, checksum: f89749f6edfe0cf5ba3776a5d131d1b2 (MD5) Previous issue date: 2013SalvadorDiagnostic Microbiology and Infectious Diseasehttp://dx.doi.org/10.1016/j.diagmicrobio.2013.01.016reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAStrongyloides stercoralisSodium metaperiodateSerodiagnosisGlycoproteinsThe role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infectionDiagnostic Microbiology and Infectious Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/11969/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINAL33333333.pdf33333333.pdfapplication/pdf306976https://repositorio.ufba.br/bitstream/ri/11969/1/33333333.pdff89749f6edfe0cf5ba3776a5d131d1b2MD51TEXT33333333.pdf.txt33333333.pdf.txtExtracted texttext/plain33945https://repositorio.ufba.br/bitstream/ri/11969/3/33333333.pdf.txtc089a5731af2af5071a269ca7bdc0951MD53ri/119692022-08-08 13:18:15.957oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:18:15Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
dc.title.alternative.pt_BR.fl_str_mv Diagnostic Microbiology and Infectious Disease
title The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
spellingShingle The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
Inês, Elizabete de Jesus
Strongyloides stercoralis
Sodium metaperiodate
Serodiagnosis
Glycoproteins
title_short The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
title_full The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
title_fullStr The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
title_full_unstemmed The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
title_sort The role of glycosylated epitopes in the serodiagnosis of Strongyloides stercoralis infection
author Inês, Elizabete de Jesus
author_facet Inês, Elizabete de Jesus
Silva, Mônica Lopes Sampaio
Souza, Joelma Nascimento
Teixeira, Márcia Cristina Aquino
Soares, Neci Matos
author_role author
author2 Silva, Mônica Lopes Sampaio
Souza, Joelma Nascimento
Teixeira, Márcia Cristina Aquino
Soares, Neci Matos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Inês, Elizabete de Jesus
Silva, Mônica Lopes Sampaio
Souza, Joelma Nascimento
Teixeira, Márcia Cristina Aquino
Soares, Neci Matos
Inês, Elizabete de Jesus
Silva, Mônica Lopes Sampaio
Souza, Joelma Nascimento
Teixeira, Márcia Cristina Aquino
Soares, Neci Matos
dc.subject.por.fl_str_mv Strongyloides stercoralis
Sodium metaperiodate
Serodiagnosis
Glycoproteins
topic Strongyloides stercoralis
Sodium metaperiodate
Serodiagnosis
Glycoproteins
description Texto completo. Acesso restrito. p. 31-35
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-06-18T20:41:48Z
dc.date.available.fl_str_mv 2013-06-18T20:41:48Z
dc.date.issued.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/11969
dc.identifier.issn.none.fl_str_mv 0732-8893
dc.identifier.number.pt_BR.fl_str_mv v. 76, n. 1
identifier_str_mv 0732-8893
v. 76, n. 1
url http://www.repositorio.ufba.br/ri/handle/ri/11969
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Diagnostic Microbiology and Infectious Disease
publisher.none.fl_str_mv Diagnostic Microbiology and Infectious Disease
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.diagmicrobio.2013.01.016
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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collection Repositório Institucional da UFBA
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