From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library

Detalhes bibliográficos
Autor(a) principal: Oliveira, Fabiano
Data de Publicação: 2006
Outros Autores: Kamhawi, Shaden, Seitz, Amy E., Pham, Van My, Guigal, Pierre M., Fischer, Laurent, Ward, Jerrold, Valenzuela, Jesus G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13681
Resumo: Texto completo: acesso restrito. p. 374–390
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spelling Oliveira, FabianoKamhawi, ShadenSeitz, Amy E.Pham, Van MyGuigal, Pierre M.Fischer, LaurentWard, JerroldValenzuela, Jesus G.Oliveira, FabianoKamhawi, ShadenSeitz, Amy E.Pham, Van MyGuigal, Pierre M.Fischer, LaurentWard, JerroldValenzuela, Jesus G.2013-11-16T13:14:34Z20060264-410Xhttp://repositorio.ufba.br/ri/handle/ri/13681v. 24, n. 3Texto completo: acesso restrito. p. 374–390Delayed-type hypersensitivity (DTH) response to arthropod vector salivary proteins is associated with protection against pathogen transmission. Massive cDNA sequencing, high-throughput DNA plasmid construction and DNA immunisation were used to identify twelve DTH inducing proteins isolated from a Phlebotomus ariasi salivary gland cDNA library. Additionally, nine P. ariasi DNA plasmids produced specific anti-saliva antibodies, four of these showed a Th1 immune response while the other two exhibited a Th2 profile as determined by IgG2a and IgG1 isotype switching, respectively. In order to validate the specificity of sand fly DNA plasmids, mice previously exposed to sand fly saliva were intradermally injected once with selected P. ariasi plasmids and a specific DTH response consisting of infiltration of mononuclear cells in varying proportions was observed at 24 and 48 h. This approach can help to identify DTH inducing proteins that may be related to host protection against vector-borne diseases or other disease agents where cellular immune response is protective.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-09-04T12:44:33Z No. of bitstreams: 1 1-s2.0-S0264410X05007498-main.pdf: 1557023 bytes, checksum: 3dc09a1a592f8a218598368d50d4f03b (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-16T13:14:34Z (GMT) No. of bitstreams: 1 1-s2.0-S0264410X05007498-main.pdf: 1557023 bytes, checksum: 3dc09a1a592f8a218598368d50d4f03b (MD5)Made available in DSpace on 2013-11-16T13:14:34Z (GMT). No. of bitstreams: 1 1-s2.0-S0264410X05007498-main.pdf: 1557023 bytes, checksum: 3dc09a1a592f8a218598368d50d4f03b (MD5) Previous issue date: 2006http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.vaccine.2005.07.085reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACellular immune responsesDNA vaccineDelayed-type hypersensitivityPhlebotomusReverse antigen screeningSand fly salivaSand fly transcriptsFrom transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA libraryVaccineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S0264410X05007498-main.pdf1-s2.0-S0264410X05007498-main.pdfapplication/pdf1557023https://repositorio.ufba.br/bitstream/ri/13681/1/1-s2.0-S0264410X05007498-main.pdf3dc09a1a592f8a218598368d50d4f03bMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13681/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S0264410X05007498-main.pdf.txt1-s2.0-S0264410X05007498-main.pdf.txtExtracted texttext/plain65524https://repositorio.ufba.br/bitstream/ri/13681/3/1-s2.0-S0264410X05007498-main.pdf.txt317c3c34b4dff7079d6911d6de6b083aMD53ri/136812022-07-05 14:02:58.307oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:58Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
dc.title.alternative.pt_BR.fl_str_mv Vaccine
title From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
spellingShingle From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
Oliveira, Fabiano
Cellular immune responses
DNA vaccine
Delayed-type hypersensitivity
Phlebotomus
Reverse antigen screening
Sand fly saliva
Sand fly transcripts
title_short From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
title_full From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
title_fullStr From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
title_full_unstemmed From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
title_sort From transcriptome to immunome: Identification of DTH inducing proteins from a Phlebotomus ariasi salivary gland cDNA library
author Oliveira, Fabiano
author_facet Oliveira, Fabiano
Kamhawi, Shaden
Seitz, Amy E.
Pham, Van My
Guigal, Pierre M.
Fischer, Laurent
Ward, Jerrold
Valenzuela, Jesus G.
author_role author
author2 Kamhawi, Shaden
Seitz, Amy E.
Pham, Van My
Guigal, Pierre M.
Fischer, Laurent
Ward, Jerrold
Valenzuela, Jesus G.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Fabiano
Kamhawi, Shaden
Seitz, Amy E.
Pham, Van My
Guigal, Pierre M.
Fischer, Laurent
Ward, Jerrold
Valenzuela, Jesus G.
Oliveira, Fabiano
Kamhawi, Shaden
Seitz, Amy E.
Pham, Van My
Guigal, Pierre M.
Fischer, Laurent
Ward, Jerrold
Valenzuela, Jesus G.
dc.subject.por.fl_str_mv Cellular immune responses
DNA vaccine
Delayed-type hypersensitivity
Phlebotomus
Reverse antigen screening
Sand fly saliva
Sand fly transcripts
topic Cellular immune responses
DNA vaccine
Delayed-type hypersensitivity
Phlebotomus
Reverse antigen screening
Sand fly saliva
Sand fly transcripts
description Texto completo: acesso restrito. p. 374–390
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2013-11-16T13:14:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13681
dc.identifier.issn.none.fl_str_mv 0264-410X
dc.identifier.number.pt_BR.fl_str_mv v. 24, n. 3
identifier_str_mv 0264-410X
v. 24, n. 3
url http://repositorio.ufba.br/ri/handle/ri/13681
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.vaccine.2005.07.085
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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