Use of protein antigens for early serological diagnosis of leprosy

Detalhes bibliográficos
Autor(a) principal: Duthie, Malcolm S.
Data de Publicação: 2007
Outros Autores: Goto, Wakako, Ireton, Greg C., Stefani, Mariane M. A., Nakatani, Maria, Jesus, Robson Crusue de, Martins Netto, Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14299
Resumo: Texto completo: acesso restrito. p. 1400-1408
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spelling Duthie, Malcolm S.Goto, WakakoIreton, Greg C.Stefani, Mariane M. A.Nakatani, MariaJesus, Robson Crusue deMartins Netto, EduardoDuthie, Malcolm S.Goto, WakakoIreton, Greg C.Stefani, Mariane M. A.Nakatani, MariaJesus, Robson Crusue deMartins Netto, Eduardo2014-01-08T13:45:42Z20071556-6811http://repositorio.ufba.br/ri/handle/ri/14299v. 14, n. 11Texto completo: acesso restrito. p. 1400-1408Leprosy is a chronic and debilitating human disease caused by infection with the Mycobacterium leprae bacillus. Despite the marked reduction in the number of registered worldwide leprosy cases as a result of the widespread use of multidrug therapy, the number of new cases detected each year remains relatively stable. This indicates that M. leprae is still being transmitted and that, without earlier diagnosis, M. leprae infection will continue to pose a health problem. Current diagnostic techniques, based on the appearance of clinical symptoms or of immunoglobulin M (IgM) antibodies that recognize the bacterial phenolic glycolipid I, are unable to reliably identify early-stage leprosy. In this study we examine the ability of IgG within leprosy patient sera to bind several M. leprae protein antigens. As expected, multibacillary leprosy patients provided stronger responses than paucibacillary leprosy patients. We demonstrate that the geographic locations of the patients can influence the antigens they recognize but that ML0405 and ML2331 are recognized by sera from diverse regions (the Philippines, coastal and central Brazil, and Japan). A fusion construct of these two proteins (designated leprosy IDRI diagnostic 1 [LID-1]) retained the diagnostic activity of the component antigens. Upon testing against a panel of prospective sera from individuals who developed leprosy, we determined that LID-1 was capable of diagnosing leprosy 6 to 8 months before the onset of clinical symptoms. A serological diagnostic test capable of identifying and allowing treatment of early-stage leprosy could reduce transmission, prevent functional disabilities and stigmatizing deformities, and facilitate leprosy eradication.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-08T13:45:42Z No. of bitstreams: 1 10.1128CVI.00299-07.pdf: 277985 bytes, checksum: 8bb4dd226c4ef0de556b3a9d860593ea (MD5)Made available in DSpace on 2014-01-08T13:45:42Z (GMT). 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dc.title.pt_BR.fl_str_mv Use of protein antigens for early serological diagnosis of leprosy
dc.title.alternative.pt_BR.fl_str_mv Clinical and Vaccine Immunology
title Use of protein antigens for early serological diagnosis of leprosy
spellingShingle Use of protein antigens for early serological diagnosis of leprosy
Duthie, Malcolm S.
title_short Use of protein antigens for early serological diagnosis of leprosy
title_full Use of protein antigens for early serological diagnosis of leprosy
title_fullStr Use of protein antigens for early serological diagnosis of leprosy
title_full_unstemmed Use of protein antigens for early serological diagnosis of leprosy
title_sort Use of protein antigens for early serological diagnosis of leprosy
author Duthie, Malcolm S.
author_facet Duthie, Malcolm S.
Goto, Wakako
Ireton, Greg C.
Stefani, Mariane M. A.
Nakatani, Maria
Jesus, Robson Crusue de
Martins Netto, Eduardo
author_role author
author2 Goto, Wakako
Ireton, Greg C.
Stefani, Mariane M. A.
Nakatani, Maria
Jesus, Robson Crusue de
Martins Netto, Eduardo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Duthie, Malcolm S.
Goto, Wakako
Ireton, Greg C.
Stefani, Mariane M. A.
Nakatani, Maria
Jesus, Robson Crusue de
Martins Netto, Eduardo
Duthie, Malcolm S.
Goto, Wakako
Ireton, Greg C.
Stefani, Mariane M. A.
Nakatani, Maria
Jesus, Robson Crusue de
Martins Netto, Eduardo
description Texto completo: acesso restrito. p. 1400-1408
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-01-08T13:45:42Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14299
dc.identifier.issn.none.fl_str_mv 1556-6811
dc.identifier.number.pt_BR.fl_str_mv v. 14, n. 11
identifier_str_mv 1556-6811
v. 14, n. 11
url http://repositorio.ufba.br/ri/handle/ri/14299
dc.language.iso.fl_str_mv eng
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