Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein

Detalhes bibliográficos
Autor(a) principal: Pinho,Ricardo Martins Araujo
Data de Publicação: 2017
Outros Autores: Santos,Edson Mauro, Oliveira,Juliana Silva de, Loureiro,Alexandre Henrique Remigio, Macêdo,Alberto Jefferson da Silva, Alves,Joyce Pereira, Santos,Ana Paula Maia dos, Santos,Vinicius da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Saúde e Produção Animal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000400505
Resumo: SUMMARY The aim of this study was to evaluate the effect of the levels of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein. A completely randomized experimental design was adopted with a 5 × 3 factorial arrangement consisting of five levels of spineless-cactus mucilage (0, 5, 10, 20, and 40%) and three substrates (carboxymethylcellulose, starch, and trypticase). Treatments were evaluated in a ruminal environment simulated by in vitro incubation at different times of assessment: 0, 3, 6, 12, 24, and 48 h. The incubation procedure was repeated three times, totaling three evaluations per incubation time for each treatment. There was an interaction (P<0.05) between the mucilage levels and substrate for all evaluated ruminal parameters, except for the concentration of microbial protein after 48 h of fermentation and for the proportions of acetate and butyrate fermentation at time 0 h. There was a quadratic increase (P<0.05) in the concentration of ammoniacal nitrogen after 48 h of incubation in the media containing carboxymethylcellulose and trypticase. pH values decreased quadratically (P<0.05) as a function of the mucilage levels in the media containing carboxymethylcellulose and trypticase. Overall, no expressive alterations were observed between the individual molar proportions of acetate, propionate, and butyrate with the addition of spineless-cactus mucilage levels to the different substrates. Spineless- cactus mucilage affects the pattern of fermentation of starch, cellulase, and protein performed by rumen microorganisms.
id UFBA-3_26792cdcebf6af9c716ff67137783a8b
oai_identifier_str oai:scielo:S1519-99402017000400505
network_acronym_str UFBA-3
network_name_str Revista Brasileira de Saúde e Produção Animal
repository_id_str
spelling Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and proteinammoniacal nitrogenmicrobial proteinpHrumen microorganismsvolatile fatty acidsSUMMARY The aim of this study was to evaluate the effect of the levels of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein. A completely randomized experimental design was adopted with a 5 × 3 factorial arrangement consisting of five levels of spineless-cactus mucilage (0, 5, 10, 20, and 40%) and three substrates (carboxymethylcellulose, starch, and trypticase). Treatments were evaluated in a ruminal environment simulated by in vitro incubation at different times of assessment: 0, 3, 6, 12, 24, and 48 h. The incubation procedure was repeated three times, totaling three evaluations per incubation time for each treatment. There was an interaction (P<0.05) between the mucilage levels and substrate for all evaluated ruminal parameters, except for the concentration of microbial protein after 48 h of fermentation and for the proportions of acetate and butyrate fermentation at time 0 h. There was a quadratic increase (P<0.05) in the concentration of ammoniacal nitrogen after 48 h of incubation in the media containing carboxymethylcellulose and trypticase. pH values decreased quadratically (P<0.05) as a function of the mucilage levels in the media containing carboxymethylcellulose and trypticase. Overall, no expressive alterations were observed between the individual molar proportions of acetate, propionate, and butyrate with the addition of spineless-cactus mucilage levels to the different substrates. Spineless- cactus mucilage affects the pattern of fermentation of starch, cellulase, and protein performed by rumen microorganisms.UFBA - Universidade Federal da Bahia2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000400505Revista Brasileira de Saúde e Produção Animal v.18 n.4 2017reponame:Revista Brasileira de Saúde e Produção Animalinstname:Universidade Federal da Bahia (UFBA)instacron:UFBA10.1590/s1519-99402017000400002info:eu-repo/semantics/openAccessPinho,Ricardo Martins AraujoSantos,Edson MauroOliveira,Juliana Silva deLoureiro,Alexandre Henrique RemigioMacêdo,Alberto Jefferson da SilvaAlves,Joyce PereiraSantos,Ana Paula Maia dosSantos,Vinicius da Silvaeng2018-01-23T00:00:00Zoai:scielo:S1519-99402017000400505Revistahttp://www.rbspa.ufba.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||rbspa@ufba.br1519-99401519-9940opendoar:2018-01-23T00:00Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA)false
dc.title.none.fl_str_mv Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
title Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
spellingShingle Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
Pinho,Ricardo Martins Araujo
ammoniacal nitrogen
microbial protein
pH
rumen microorganisms
volatile fatty acids
title_short Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
title_full Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
title_fullStr Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
title_full_unstemmed Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
title_sort Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein
author Pinho,Ricardo Martins Araujo
author_facet Pinho,Ricardo Martins Araujo
Santos,Edson Mauro
Oliveira,Juliana Silva de
Loureiro,Alexandre Henrique Remigio
Macêdo,Alberto Jefferson da Silva
Alves,Joyce Pereira
Santos,Ana Paula Maia dos
Santos,Vinicius da Silva
author_role author
author2 Santos,Edson Mauro
Oliveira,Juliana Silva de
Loureiro,Alexandre Henrique Remigio
Macêdo,Alberto Jefferson da Silva
Alves,Joyce Pereira
Santos,Ana Paula Maia dos
Santos,Vinicius da Silva
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinho,Ricardo Martins Araujo
Santos,Edson Mauro
Oliveira,Juliana Silva de
Loureiro,Alexandre Henrique Remigio
Macêdo,Alberto Jefferson da Silva
Alves,Joyce Pereira
Santos,Ana Paula Maia dos
Santos,Vinicius da Silva
dc.subject.por.fl_str_mv ammoniacal nitrogen
microbial protein
pH
rumen microorganisms
volatile fatty acids
topic ammoniacal nitrogen
microbial protein
pH
rumen microorganisms
volatile fatty acids
description SUMMARY The aim of this study was to evaluate the effect of the levels of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein. A completely randomized experimental design was adopted with a 5 × 3 factorial arrangement consisting of five levels of spineless-cactus mucilage (0, 5, 10, 20, and 40%) and three substrates (carboxymethylcellulose, starch, and trypticase). Treatments were evaluated in a ruminal environment simulated by in vitro incubation at different times of assessment: 0, 3, 6, 12, 24, and 48 h. The incubation procedure was repeated three times, totaling three evaluations per incubation time for each treatment. There was an interaction (P<0.05) between the mucilage levels and substrate for all evaluated ruminal parameters, except for the concentration of microbial protein after 48 h of fermentation and for the proportions of acetate and butyrate fermentation at time 0 h. There was a quadratic increase (P<0.05) in the concentration of ammoniacal nitrogen after 48 h of incubation in the media containing carboxymethylcellulose and trypticase. pH values decreased quadratically (P<0.05) as a function of the mucilage levels in the media containing carboxymethylcellulose and trypticase. Overall, no expressive alterations were observed between the individual molar proportions of acetate, propionate, and butyrate with the addition of spineless-cactus mucilage levels to the different substrates. Spineless- cactus mucilage affects the pattern of fermentation of starch, cellulase, and protein performed by rumen microorganisms.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000400505
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000400505
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1519-99402017000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv UFBA - Universidade Federal da Bahia
publisher.none.fl_str_mv UFBA - Universidade Federal da Bahia
dc.source.none.fl_str_mv Revista Brasileira de Saúde e Produção Animal v.18 n.4 2017
reponame:Revista Brasileira de Saúde e Produção Animal
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Revista Brasileira de Saúde e Produção Animal
collection Revista Brasileira de Saúde e Produção Animal
repository.name.fl_str_mv Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv ||rbspa@ufba.br
_version_ 1750297508238589952