Detection of agents associated with respiratory diseases of swine by real time PCR
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Saúde e Produção Animal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402015000200005 |
Resumo: | Porcine Respiratory Disease Complex (PRDC) is a group of diseases that cause high losses in the swine industry. Several infectious agents are related to PRDC including porcine circovirus 2 (PCV-2), pseudorabies virus (SuHV-1),Haemophilus parasuis (HP), Mycoplasma hypneumoniae (MH) and Pasteurela multocida (PM). The aim of this study was to develop real-time PCRs (qPCR) for the detection of these infectious agents. Oligonucleotides were designed for each specific infectious agent and labeled with different fluorophores to amplify specific parts of the genome. This was done in two groups of reactions—a duplex qPCR for SuHV-1 and PCV-2 and a multiplex qPCR to detect the three bacteria simultaneously. The reactions were tested in 142 pooled samples of swine lymph nodes and lungs with clinical signs of PRDC. There were 135 samples that tested positive for PCV-2, 61 for HP, 29 for PM, 30 for MH and zero for SuHV-1. We recorded 76 cases of co-infection. The qPCRs developed in this study are useful tools in the diagnosis of PRDC. |
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Detection of agents associated with respiratory diseases of swine by real time PCRmolecular biologypreventive veterinary medicinevirusesPorcine Respiratory Disease Complex (PRDC) is a group of diseases that cause high losses in the swine industry. Several infectious agents are related to PRDC including porcine circovirus 2 (PCV-2), pseudorabies virus (SuHV-1),Haemophilus parasuis (HP), Mycoplasma hypneumoniae (MH) and Pasteurela multocida (PM). The aim of this study was to develop real-time PCRs (qPCR) for the detection of these infectious agents. Oligonucleotides were designed for each specific infectious agent and labeled with different fluorophores to amplify specific parts of the genome. This was done in two groups of reactions—a duplex qPCR for SuHV-1 and PCV-2 and a multiplex qPCR to detect the three bacteria simultaneously. The reactions were tested in 142 pooled samples of swine lymph nodes and lungs with clinical signs of PRDC. There were 135 samples that tested positive for PCV-2, 61 for HP, 29 for PM, 30 for MH and zero for SuHV-1. We recorded 76 cases of co-infection. The qPCRs developed in this study are useful tools in the diagnosis of PRDC.UFBA - Universidade Federal da Bahia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402015000200005Revista Brasileira de Saúde e Produção Animal v.16 n.2 2015reponame:Revista Brasileira de Saúde e Produção Animalinstname:Universidade Federal da Bahia (UFBA)instacron:UFBA10.1590/S1519-99402015000200005info:eu-repo/semantics/openAccessFONSECA JUNIOR,Antônio AugustoNONAKA,Carolina Kymie VasquezGUEDES,Estefânia de OliveiraLOBATO,Zélia Inês PortelaDIAS,Alessandra SilvaNASCIMENTO,Juliana Amália Fonte Boa doKLEIN,Cátia SileneREIS,Jenner Karlisson Pimenta dosHEINEMANN,Marcos Bryaneng2015-09-23T00:00:00Zoai:scielo:S1519-99402015000200005Revistahttp://www.rbspa.ufba.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||rbspa@ufba.br1519-99401519-9940opendoar:2015-09-23T00:00Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA)false |
dc.title.none.fl_str_mv |
Detection of agents associated with respiratory diseases of swine by real time PCR |
title |
Detection of agents associated with respiratory diseases of swine by real time PCR |
spellingShingle |
Detection of agents associated with respiratory diseases of swine by real time PCR FONSECA JUNIOR,Antônio Augusto molecular biology preventive veterinary medicine viruses |
title_short |
Detection of agents associated with respiratory diseases of swine by real time PCR |
title_full |
Detection of agents associated with respiratory diseases of swine by real time PCR |
title_fullStr |
Detection of agents associated with respiratory diseases of swine by real time PCR |
title_full_unstemmed |
Detection of agents associated with respiratory diseases of swine by real time PCR |
title_sort |
Detection of agents associated with respiratory diseases of swine by real time PCR |
author |
FONSECA JUNIOR,Antônio Augusto |
author_facet |
FONSECA JUNIOR,Antônio Augusto NONAKA,Carolina Kymie Vasquez GUEDES,Estefânia de Oliveira LOBATO,Zélia Inês Portela DIAS,Alessandra Silva NASCIMENTO,Juliana Amália Fonte Boa do KLEIN,Cátia Silene REIS,Jenner Karlisson Pimenta dos HEINEMANN,Marcos Bryan |
author_role |
author |
author2 |
NONAKA,Carolina Kymie Vasquez GUEDES,Estefânia de Oliveira LOBATO,Zélia Inês Portela DIAS,Alessandra Silva NASCIMENTO,Juliana Amália Fonte Boa do KLEIN,Cátia Silene REIS,Jenner Karlisson Pimenta dos HEINEMANN,Marcos Bryan |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
FONSECA JUNIOR,Antônio Augusto NONAKA,Carolina Kymie Vasquez GUEDES,Estefânia de Oliveira LOBATO,Zélia Inês Portela DIAS,Alessandra Silva NASCIMENTO,Juliana Amália Fonte Boa do KLEIN,Cátia Silene REIS,Jenner Karlisson Pimenta dos HEINEMANN,Marcos Bryan |
dc.subject.por.fl_str_mv |
molecular biology preventive veterinary medicine viruses |
topic |
molecular biology preventive veterinary medicine viruses |
description |
Porcine Respiratory Disease Complex (PRDC) is a group of diseases that cause high losses in the swine industry. Several infectious agents are related to PRDC including porcine circovirus 2 (PCV-2), pseudorabies virus (SuHV-1),Haemophilus parasuis (HP), Mycoplasma hypneumoniae (MH) and Pasteurela multocida (PM). The aim of this study was to develop real-time PCRs (qPCR) for the detection of these infectious agents. Oligonucleotides were designed for each specific infectious agent and labeled with different fluorophores to amplify specific parts of the genome. This was done in two groups of reactions—a duplex qPCR for SuHV-1 and PCV-2 and a multiplex qPCR to detect the three bacteria simultaneously. The reactions were tested in 142 pooled samples of swine lymph nodes and lungs with clinical signs of PRDC. There were 135 samples that tested positive for PCV-2, 61 for HP, 29 for PM, 30 for MH and zero for SuHV-1. We recorded 76 cases of co-infection. The qPCRs developed in this study are useful tools in the diagnosis of PRDC. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402015000200005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402015000200005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1519-99402015000200005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
UFBA - Universidade Federal da Bahia |
publisher.none.fl_str_mv |
UFBA - Universidade Federal da Bahia |
dc.source.none.fl_str_mv |
Revista Brasileira de Saúde e Produção Animal v.16 n.2 2015 reponame:Revista Brasileira de Saúde e Produção Animal instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
instname_str |
Universidade Federal da Bahia (UFBA) |
instacron_str |
UFBA |
institution |
UFBA |
reponame_str |
Revista Brasileira de Saúde e Produção Animal |
collection |
Revista Brasileira de Saúde e Produção Animal |
repository.name.fl_str_mv |
Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA) |
repository.mail.fl_str_mv |
||rbspa@ufba.br |
_version_ |
1750297507623075840 |