Detection of agents associated with respiratory diseases of swine by real time PCR

Detalhes bibliográficos
Autor(a) principal: FONSECA JUNIOR,Antônio Augusto
Data de Publicação: 2015
Outros Autores: NONAKA,Carolina Kymie Vasquez, GUEDES,Estefânia de Oliveira, LOBATO,Zélia Inês Portela, DIAS,Alessandra Silva, NASCIMENTO,Juliana Amália Fonte Boa do, KLEIN,Cátia Silene, REIS,Jenner Karlisson Pimenta dos, HEINEMANN,Marcos Bryan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Saúde e Produção Animal
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402015000200005
Resumo: Porcine Respiratory Disease Complex (PRDC) is a group of diseases that cause high losses in the swine industry. Several infectious agents are related to PRDC including porcine circovirus 2 (PCV-2), pseudorabies virus (SuHV-1),Haemophilus parasuis (HP), Mycoplasma hypneumoniae (MH) and Pasteurela multocida (PM). The aim of this study was to develop real-time PCRs (qPCR) for the detection of these infectious agents. Oligonucleotides were designed for each specific infectious agent and labeled with different fluorophores to amplify specific parts of the genome. This was done in two groups of reactions—a duplex qPCR for SuHV-1 and PCV-2 and a multiplex qPCR to detect the three bacteria simultaneously. The reactions were tested in 142 pooled samples of swine lymph nodes and lungs with clinical signs of PRDC. There were 135 samples that tested positive for PCV-2, 61 for HP, 29 for PM, 30 for MH and zero for SuHV-1. We recorded 76 cases of co-infection. The qPCRs developed in this study are useful tools in the diagnosis of PRDC.
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spelling Detection of agents associated with respiratory diseases of swine by real time PCRmolecular biologypreventive veterinary medicinevirusesPorcine Respiratory Disease Complex (PRDC) is a group of diseases that cause high losses in the swine industry. Several infectious agents are related to PRDC including porcine circovirus 2 (PCV-2), pseudorabies virus (SuHV-1),Haemophilus parasuis (HP), Mycoplasma hypneumoniae (MH) and Pasteurela multocida (PM). The aim of this study was to develop real-time PCRs (qPCR) for the detection of these infectious agents. Oligonucleotides were designed for each specific infectious agent and labeled with different fluorophores to amplify specific parts of the genome. This was done in two groups of reactions—a duplex qPCR for SuHV-1 and PCV-2 and a multiplex qPCR to detect the three bacteria simultaneously. The reactions were tested in 142 pooled samples of swine lymph nodes and lungs with clinical signs of PRDC. There were 135 samples that tested positive for PCV-2, 61 for HP, 29 for PM, 30 for MH and zero for SuHV-1. We recorded 76 cases of co-infection. The qPCRs developed in this study are useful tools in the diagnosis of PRDC.UFBA - Universidade Federal da Bahia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402015000200005Revista Brasileira de Saúde e Produção Animal v.16 n.2 2015reponame:Revista Brasileira de Saúde e Produção Animalinstname:Universidade Federal da Bahia (UFBA)instacron:UFBA10.1590/S1519-99402015000200005info:eu-repo/semantics/openAccessFONSECA JUNIOR,Antônio AugustoNONAKA,Carolina Kymie VasquezGUEDES,Estefânia de OliveiraLOBATO,Zélia Inês PortelaDIAS,Alessandra SilvaNASCIMENTO,Juliana Amália Fonte Boa doKLEIN,Cátia SileneREIS,Jenner Karlisson Pimenta dosHEINEMANN,Marcos Bryaneng2015-09-23T00:00:00Zoai:scielo:S1519-99402015000200005Revistahttp://www.rbspa.ufba.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||rbspa@ufba.br1519-99401519-9940opendoar:2015-09-23T00:00Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA)false
dc.title.none.fl_str_mv Detection of agents associated with respiratory diseases of swine by real time PCR
title Detection of agents associated with respiratory diseases of swine by real time PCR
spellingShingle Detection of agents associated with respiratory diseases of swine by real time PCR
FONSECA JUNIOR,Antônio Augusto
molecular biology
preventive veterinary medicine
viruses
title_short Detection of agents associated with respiratory diseases of swine by real time PCR
title_full Detection of agents associated with respiratory diseases of swine by real time PCR
title_fullStr Detection of agents associated with respiratory diseases of swine by real time PCR
title_full_unstemmed Detection of agents associated with respiratory diseases of swine by real time PCR
title_sort Detection of agents associated with respiratory diseases of swine by real time PCR
author FONSECA JUNIOR,Antônio Augusto
author_facet FONSECA JUNIOR,Antônio Augusto
NONAKA,Carolina Kymie Vasquez
GUEDES,Estefânia de Oliveira
LOBATO,Zélia Inês Portela
DIAS,Alessandra Silva
NASCIMENTO,Juliana Amália Fonte Boa do
KLEIN,Cátia Silene
REIS,Jenner Karlisson Pimenta dos
HEINEMANN,Marcos Bryan
author_role author
author2 NONAKA,Carolina Kymie Vasquez
GUEDES,Estefânia de Oliveira
LOBATO,Zélia Inês Portela
DIAS,Alessandra Silva
NASCIMENTO,Juliana Amália Fonte Boa do
KLEIN,Cátia Silene
REIS,Jenner Karlisson Pimenta dos
HEINEMANN,Marcos Bryan
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv FONSECA JUNIOR,Antônio Augusto
NONAKA,Carolina Kymie Vasquez
GUEDES,Estefânia de Oliveira
LOBATO,Zélia Inês Portela
DIAS,Alessandra Silva
NASCIMENTO,Juliana Amália Fonte Boa do
KLEIN,Cátia Silene
REIS,Jenner Karlisson Pimenta dos
HEINEMANN,Marcos Bryan
dc.subject.por.fl_str_mv molecular biology
preventive veterinary medicine
viruses
topic molecular biology
preventive veterinary medicine
viruses
description Porcine Respiratory Disease Complex (PRDC) is a group of diseases that cause high losses in the swine industry. Several infectious agents are related to PRDC including porcine circovirus 2 (PCV-2), pseudorabies virus (SuHV-1),Haemophilus parasuis (HP), Mycoplasma hypneumoniae (MH) and Pasteurela multocida (PM). The aim of this study was to develop real-time PCRs (qPCR) for the detection of these infectious agents. Oligonucleotides were designed for each specific infectious agent and labeled with different fluorophores to amplify specific parts of the genome. This was done in two groups of reactions—a duplex qPCR for SuHV-1 and PCV-2 and a multiplex qPCR to detect the three bacteria simultaneously. The reactions were tested in 142 pooled samples of swine lymph nodes and lungs with clinical signs of PRDC. There were 135 samples that tested positive for PCV-2, 61 for HP, 29 for PM, 30 for MH and zero for SuHV-1. We recorded 76 cases of co-infection. The qPCRs developed in this study are useful tools in the diagnosis of PRDC.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402015000200005
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1519-99402015000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv UFBA - Universidade Federal da Bahia
publisher.none.fl_str_mv UFBA - Universidade Federal da Bahia
dc.source.none.fl_str_mv Revista Brasileira de Saúde e Produção Animal v.16 n.2 2015
reponame:Revista Brasileira de Saúde e Produção Animal
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
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institution UFBA
reponame_str Revista Brasileira de Saúde e Produção Animal
collection Revista Brasileira de Saúde e Produção Animal
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