Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Saúde e Produção Animal |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000100211 |
Resumo: | SUMMARY This study aimed to identify proteins in the seminal plasma associated with fertility in sheep of Santa Inês in Manaus, AM, using twodimensional electrophoresis techniques associated with mass spectrometry. Semen samples from eight adult sheep were collected by removing an aliquot for the physical and morphological assessments of semen and seminal plasma was subjected to SDS-PAGE profile and two-dimensional electrophoresis. Gels were stained with colloidal Coomassie, scanned and analyzed using ImageMaster 2D Platinum software, version 6.0. The selected individual spots were cut from the master gel, digested with trypsin and subjected to identification by mass spectrometry (MALDITof / Tof). Of the 108 spots detected in the gel, it selected 10 differential spots (based on the distribution thereof in the bidimensional gel and pre-analysis of the 2D ImageMaster Platinum Software) identifying 03 proteins: clusterin, a protein 14-3-3 zeta chain and Ram Seminal versicles 22kDa Protein. The identity of these proteins implies that the components of seminal plasma participate in physiological processes involved in sperm protection, motility and sperm capacitation, all associated with fertility. These proteins need to be better studied to see whether the same could be used as molecular markers of fertility as they were also found in other studies conducted with sheep Santa Ines. |
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Revista Brasileira de Saúde e Produção Animal |
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Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheepfertilityseminal plasmatwo-dimensional electrophoresisSUMMARY This study aimed to identify proteins in the seminal plasma associated with fertility in sheep of Santa Inês in Manaus, AM, using twodimensional electrophoresis techniques associated with mass spectrometry. Semen samples from eight adult sheep were collected by removing an aliquot for the physical and morphological assessments of semen and seminal plasma was subjected to SDS-PAGE profile and two-dimensional electrophoresis. Gels were stained with colloidal Coomassie, scanned and analyzed using ImageMaster 2D Platinum software, version 6.0. The selected individual spots were cut from the master gel, digested with trypsin and subjected to identification by mass spectrometry (MALDITof / Tof). Of the 108 spots detected in the gel, it selected 10 differential spots (based on the distribution thereof in the bidimensional gel and pre-analysis of the 2D ImageMaster Platinum Software) identifying 03 proteins: clusterin, a protein 14-3-3 zeta chain and Ram Seminal versicles 22kDa Protein. The identity of these proteins implies that the components of seminal plasma participate in physiological processes involved in sperm protection, motility and sperm capacitation, all associated with fertility. These proteins need to be better studied to see whether the same could be used as molecular markers of fertility as they were also found in other studies conducted with sheep Santa Ines.UFBA - Universidade Federal da Bahia2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000100211Revista Brasileira de Saúde e Produção Animal v.18 n.1 2017reponame:Revista Brasileira de Saúde e Produção Animalinstname:Universidade Federal da Bahia (UFBA)instacron:UFBA10.1590/s1519-99402017000100020info:eu-repo/semantics/openAccessOLIVEIRA,Marilza Assunção deOLIVEIRA,Roseane Pinto Martins deLIMA,Ana Rita deANDRADE,Edmar Vaz deABREU,Jan Sidarta Lima deOLIVEIRA,Franklyn Ferreira deeng2017-04-06T00:00:00Zoai:scielo:S1519-99402017000100211Revistahttp://www.rbspa.ufba.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||rbspa@ufba.br1519-99401519-9940opendoar:2017-04-06T00:00Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA)false |
dc.title.none.fl_str_mv |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep |
title |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep |
spellingShingle |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep OLIVEIRA,Marilza Assunção de fertility seminal plasma two-dimensional electrophoresis |
title_short |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep |
title_full |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep |
title_fullStr |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep |
title_full_unstemmed |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep |
title_sort |
Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep |
author |
OLIVEIRA,Marilza Assunção de |
author_facet |
OLIVEIRA,Marilza Assunção de OLIVEIRA,Roseane Pinto Martins de LIMA,Ana Rita de ANDRADE,Edmar Vaz de ABREU,Jan Sidarta Lima de OLIVEIRA,Franklyn Ferreira de |
author_role |
author |
author2 |
OLIVEIRA,Roseane Pinto Martins de LIMA,Ana Rita de ANDRADE,Edmar Vaz de ABREU,Jan Sidarta Lima de OLIVEIRA,Franklyn Ferreira de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
OLIVEIRA,Marilza Assunção de OLIVEIRA,Roseane Pinto Martins de LIMA,Ana Rita de ANDRADE,Edmar Vaz de ABREU,Jan Sidarta Lima de OLIVEIRA,Franklyn Ferreira de |
dc.subject.por.fl_str_mv |
fertility seminal plasma two-dimensional electrophoresis |
topic |
fertility seminal plasma two-dimensional electrophoresis |
description |
SUMMARY This study aimed to identify proteins in the seminal plasma associated with fertility in sheep of Santa Inês in Manaus, AM, using twodimensional electrophoresis techniques associated with mass spectrometry. Semen samples from eight adult sheep were collected by removing an aliquot for the physical and morphological assessments of semen and seminal plasma was subjected to SDS-PAGE profile and two-dimensional electrophoresis. Gels were stained with colloidal Coomassie, scanned and analyzed using ImageMaster 2D Platinum software, version 6.0. The selected individual spots were cut from the master gel, digested with trypsin and subjected to identification by mass spectrometry (MALDITof / Tof). Of the 108 spots detected in the gel, it selected 10 differential spots (based on the distribution thereof in the bidimensional gel and pre-analysis of the 2D ImageMaster Platinum Software) identifying 03 proteins: clusterin, a protein 14-3-3 zeta chain and Ram Seminal versicles 22kDa Protein. The identity of these proteins implies that the components of seminal plasma participate in physiological processes involved in sperm protection, motility and sperm capacitation, all associated with fertility. These proteins need to be better studied to see whether the same could be used as molecular markers of fertility as they were also found in other studies conducted with sheep Santa Ines. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000100211 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1519-99402017000100211 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1519-99402017000100020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
UFBA - Universidade Federal da Bahia |
publisher.none.fl_str_mv |
UFBA - Universidade Federal da Bahia |
dc.source.none.fl_str_mv |
Revista Brasileira de Saúde e Produção Animal v.18 n.1 2017 reponame:Revista Brasileira de Saúde e Produção Animal instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
instname_str |
Universidade Federal da Bahia (UFBA) |
instacron_str |
UFBA |
institution |
UFBA |
reponame_str |
Revista Brasileira de Saúde e Produção Animal |
collection |
Revista Brasileira de Saúde e Produção Animal |
repository.name.fl_str_mv |
Revista Brasileira de Saúde e Produção Animal - Universidade Federal da Bahia (UFBA) |
repository.mail.fl_str_mv |
||rbspa@ufba.br |
_version_ |
1750297508189306880 |