Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs

Detalhes bibliográficos
Autor(a) principal: Freitas,Suzane Fonseca
Data de Publicação: 2020
Outros Autores: Moreira,Heden Luiz Marques, Souza,Daiane Machado, Moreira,Carla Giovane Ávila, Tavares,Rafael Aldrighi, Dionello,Nelson José Laurino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista ciência agronômica (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409
Resumo: ABSTRACT This study aimed to develop PCR assays, reaction combinations, and validation of tetranucleotide SSR loci for tilapia, to minimize the impact of erroneous allele inferences on genotype determination of this marker. Microsatellites containing tetranucleotide repeats were obtained from tilapia genome, version 2.1, avoiding loci in the same linking group. Primers were designed for different fragment sizes, and fluorescence added to each locus. A total of 10 loci were amplified, separately and in combination, and loaded into a single capillary sequencer panel. Alleles were amplified without stutters and easily interpreted. PCR amplifications of DNA repeatedly extracted from samples, and genotyping at different PCR rounds were performed to infer allele signaling errors. The panel obtained in this study is currently used in kinship analyses and pedigree corrections in a tilapia breeding program.
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spelling Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programsGenetic variabilityGenotypingMicrosatellite markersABSTRACT This study aimed to develop PCR assays, reaction combinations, and validation of tetranucleotide SSR loci for tilapia, to minimize the impact of erroneous allele inferences on genotype determination of this marker. Microsatellites containing tetranucleotide repeats were obtained from tilapia genome, version 2.1, avoiding loci in the same linking group. Primers were designed for different fragment sizes, and fluorescence added to each locus. A total of 10 loci were amplified, separately and in combination, and loaded into a single capillary sequencer panel. Alleles were amplified without stutters and easily interpreted. PCR amplifications of DNA repeatedly extracted from samples, and genotyping at different PCR rounds were performed to infer allele signaling errors. The panel obtained in this study is currently used in kinship analyses and pedigree corrections in a tilapia breeding program.Universidade Federal do Ceará2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409Revista Ciência Agronômica v.51 n.4 2020reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFC10.5935/1806-6690.20200068info:eu-repo/semantics/openAccessFreitas,Suzane FonsecaMoreira,Heden Luiz MarquesSouza,Daiane MachadoMoreira,Carla Giovane ÁvilaTavares,Rafael AldrighiDionello,Nelson José Laurinoeng2020-10-20T00:00:00Zoai:scielo:S1806-66902020000400409Revistahttp://www.ccarevista.ufc.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2020-10-20T00:00Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
title Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
spellingShingle Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
Freitas,Suzane Fonseca
Genetic variability
Genotyping
Microsatellite markers
title_short Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
title_full Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
title_fullStr Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
title_full_unstemmed Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
title_sort Validation of SSR-tetranucleotide multiplex panel for kinship evaluation in tilapia breeding programs
author Freitas,Suzane Fonseca
author_facet Freitas,Suzane Fonseca
Moreira,Heden Luiz Marques
Souza,Daiane Machado
Moreira,Carla Giovane Ávila
Tavares,Rafael Aldrighi
Dionello,Nelson José Laurino
author_role author
author2 Moreira,Heden Luiz Marques
Souza,Daiane Machado
Moreira,Carla Giovane Ávila
Tavares,Rafael Aldrighi
Dionello,Nelson José Laurino
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Freitas,Suzane Fonseca
Moreira,Heden Luiz Marques
Souza,Daiane Machado
Moreira,Carla Giovane Ávila
Tavares,Rafael Aldrighi
Dionello,Nelson José Laurino
dc.subject.por.fl_str_mv Genetic variability
Genotyping
Microsatellite markers
topic Genetic variability
Genotyping
Microsatellite markers
description ABSTRACT This study aimed to develop PCR assays, reaction combinations, and validation of tetranucleotide SSR loci for tilapia, to minimize the impact of erroneous allele inferences on genotype determination of this marker. Microsatellites containing tetranucleotide repeats were obtained from tilapia genome, version 2.1, avoiding loci in the same linking group. Primers were designed for different fragment sizes, and fluorescence added to each locus. A total of 10 loci were amplified, separately and in combination, and loaded into a single capillary sequencer panel. Alleles were amplified without stutters and easily interpreted. PCR amplifications of DNA repeatedly extracted from samples, and genotyping at different PCR rounds were performed to infer allele signaling errors. The panel obtained in this study is currently used in kinship analyses and pedigree corrections in a tilapia breeding program.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/1806-6690.20200068
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal do Ceará
publisher.none.fl_str_mv Universidade Federal do Ceará
dc.source.none.fl_str_mv Revista Ciência Agronômica v.51 n.4 2020
reponame:Revista ciência agronômica (Online)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Revista ciência agronômica (Online)
collection Revista ciência agronômica (Online)
repository.name.fl_str_mv Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv ||alekdutra@ufc.br|| ccarev@ufc.br
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