Tetrazolium test for the viability of gherkin seeds
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista ciência agronômica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100118 |
Resumo: | ABSTRACT The tetrazolium test is a quick and effective method for evaluating viability in seeds. The aim of this study was to determine a methodology for using the tetrazolium test, and its efficiency in verifying viability in seeds of the gherkin (Cucumis anguria L.). Consequently, five batches of gherkin seed were initially evaluated by the tests for germination, first count and speed of germination. The batches were then subjected to treatments in order to adjust the methodology for the tetrazolium test, evaluating three concentrations of tetrazolium solution (0.100, 0.075 and 0.050%), kept in the dark for two, four and six hours, under temperatures of 35 °C and 40 °C, with four replications of 25 seeds per treatment. At the end of the staining period, the seeds were washed in running water, split lengthways between the cotyledons, and classified as viable and non-viable. Evaluation of viability in seeds of the gherkin by tetrazolium test is efficient when carried out at a concentration of 0.050%, either for six hours at 35 °C or four hours at 40 °C. |
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Tetrazolium test for the viability of gherkin seedsCucumis anguriaViabilityVigourQuick testABSTRACT The tetrazolium test is a quick and effective method for evaluating viability in seeds. The aim of this study was to determine a methodology for using the tetrazolium test, and its efficiency in verifying viability in seeds of the gherkin (Cucumis anguria L.). Consequently, five batches of gherkin seed were initially evaluated by the tests for germination, first count and speed of germination. The batches were then subjected to treatments in order to adjust the methodology for the tetrazolium test, evaluating three concentrations of tetrazolium solution (0.100, 0.075 and 0.050%), kept in the dark for two, four and six hours, under temperatures of 35 °C and 40 °C, with four replications of 25 seeds per treatment. At the end of the staining period, the seeds were washed in running water, split lengthways between the cotyledons, and classified as viable and non-viable. Evaluation of viability in seeds of the gherkin by tetrazolium test is efficient when carried out at a concentration of 0.050%, either for six hours at 35 °C or four hours at 40 °C.Universidade Federal do Ceará2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100118Revista Ciência Agronômica v.48 n.1 2017reponame:Revista ciência agronômica (Online)instname:Universidade Federal do Ceará (UFC)instacron:UFC10.5935/1806-6690.20170013info:eu-repo/semantics/openAccessPaiva,Emanoela Pereira deTorres,Salvador BarrosAlmeida,João Paulo Nobre deSá,Francisco Vanies da SilvaOliveira,Renata Ramayane Torquatoeng2016-11-22T00:00:00Zoai:scielo:S1806-66902017000100118Revistahttp://www.ccarevista.ufc.br/PUBhttps://old.scielo.br/oai/scielo-oai.php||alekdutra@ufc.br|| ccarev@ufc.br1806-66900045-6888opendoar:2016-11-22T00:00Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Tetrazolium test for the viability of gherkin seeds |
title |
Tetrazolium test for the viability of gherkin seeds |
spellingShingle |
Tetrazolium test for the viability of gherkin seeds Paiva,Emanoela Pereira de Cucumis anguria Viability Vigour Quick test |
title_short |
Tetrazolium test for the viability of gherkin seeds |
title_full |
Tetrazolium test for the viability of gherkin seeds |
title_fullStr |
Tetrazolium test for the viability of gherkin seeds |
title_full_unstemmed |
Tetrazolium test for the viability of gherkin seeds |
title_sort |
Tetrazolium test for the viability of gherkin seeds |
author |
Paiva,Emanoela Pereira de |
author_facet |
Paiva,Emanoela Pereira de Torres,Salvador Barros Almeida,João Paulo Nobre de Sá,Francisco Vanies da Silva Oliveira,Renata Ramayane Torquato |
author_role |
author |
author2 |
Torres,Salvador Barros Almeida,João Paulo Nobre de Sá,Francisco Vanies da Silva Oliveira,Renata Ramayane Torquato |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Paiva,Emanoela Pereira de Torres,Salvador Barros Almeida,João Paulo Nobre de Sá,Francisco Vanies da Silva Oliveira,Renata Ramayane Torquato |
dc.subject.por.fl_str_mv |
Cucumis anguria Viability Vigour Quick test |
topic |
Cucumis anguria Viability Vigour Quick test |
description |
ABSTRACT The tetrazolium test is a quick and effective method for evaluating viability in seeds. The aim of this study was to determine a methodology for using the tetrazolium test, and its efficiency in verifying viability in seeds of the gherkin (Cucumis anguria L.). Consequently, five batches of gherkin seed were initially evaluated by the tests for germination, first count and speed of germination. The batches were then subjected to treatments in order to adjust the methodology for the tetrazolium test, evaluating three concentrations of tetrazolium solution (0.100, 0.075 and 0.050%), kept in the dark for two, four and six hours, under temperatures of 35 °C and 40 °C, with four replications of 25 seeds per treatment. At the end of the staining period, the seeds were washed in running water, split lengthways between the cotyledons, and classified as viable and non-viable. Evaluation of viability in seeds of the gherkin by tetrazolium test is efficient when carried out at a concentration of 0.050%, either for six hours at 35 °C or four hours at 40 °C. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100118 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902017000100118 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/1806-6690.20170013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal do Ceará |
publisher.none.fl_str_mv |
Universidade Federal do Ceará |
dc.source.none.fl_str_mv |
Revista Ciência Agronômica v.48 n.1 2017 reponame:Revista ciência agronômica (Online) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Revista ciência agronômica (Online) |
collection |
Revista ciência agronômica (Online) |
repository.name.fl_str_mv |
Revista ciência agronômica (Online) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
||alekdutra@ufc.br|| ccarev@ufc.br |
_version_ |
1750297488535846912 |