The effects of cryoprotectants on chilled pirapitinga (Piaractus brachypomus) embryos at various ontogenetic stages

• Autor(a) principal: Pessoa, Nathalie Ommundsen; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil
• Data de Publicação: 2014
• Outros Autores: Viveiros, Ana Tereza de Mendonça; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil, Barbosa, Thales Cordeiro; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil, Souza Filho, Francisco Gerson Mendes de; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil, Filho, Aldeney Andrade Soares; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil, Sousa, Míriam Luiza Nogueira Martins de; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil, Lourenço, Athur Vinícius; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil, Sampaio, Célia Maria Souza; Dept of Biology, State University of Ceará, UECE, Fortaleza, CE, 60740-000, Brazil
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista brasileira de higiene e sanidade animal
• Texto Completo: http://www.higieneanimal.ufc.br/seer/index.php/higieneanimal/article/view/189
• topic: aquaculture; South American fish; embryonic stage; Larval survival

Cryopreservation has not been successfully used to preserve fish embryos, although chilling techniques have been used with good results. The aim of this study was to chill Piaractus brachypomus embryos at different stages of development in some cryoprotectants and for various periods of chilling. Embryos at the following ontogenetic stages were used: blastoderm – 1.2 hours post-fertilization (hpf); epiboly – 5 hpf; blastopore closure – 8 hpf; and appearance of optic vesicle – 13 hpf. One hundred embryos were selected from each of the four stages and chilled in methanol, methylglycol or dimethylsulfoxide (DMSO) for 6, 8, 10 or 12 hours, at 2ºC. The total number of treatments was 4 stages x 3 cryoprotectants x 4 periods of chilling. The highest percentage of normal and live larvae (30.6%) was observed when embryos were chilled at 13-hpf in methanol for 6 hours. In general, larvae chilled at a more developed stages (8 and 13 hpf), in methanol and for shorter periods could survive chilling and develop normally, compared to the other treatments. Therefore, P. brachypomus embryos at the optical vesicle appearance stage (13 hpf) should be chilled in a solution containing  17.5%  glucose and 10% methanol for up to eight six at 2°C.