Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco

Detalhes bibliográficos
Autor(a) principal: Lima Júnior, Roberto César Pereira
Data de Publicação: 2008
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
dARK ID: ark:/83112/001300000z1c8
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/3843
Resumo: Severe Intestinal Mucositis (IM) and diarrhea are frequent and dose-limiting side-effects of colon cancer chemotherapy with irinotecan (CPT-11). At present, much of IM pathophysiology remains unknown. Aims: To study the mechanisms and inflammatory mediators involved in CPT-11-induced intestinal mucositis, verifying the role of the protease caspase-1, cytokines interleukin-1 (IL-1), interleukin-18 (IL-18) and interleukin-33 (IL-33), nitric oxide, 5-lipoxygenase and PAF as well as the sequence of activation of these mediators in the inflammatory response and morphofunctional contexts. Methods: Swiss, C57BL/6 (BL), BALB/c (BC) male mice or caspases-1(Casp-1-/-), IL-18 (IL-18-/-), inducible nitric oxide synthase (iNOS-/-), 5-Lipoxygenase (5-LOX-/-), PAF receptor (PAFr-/-) knockout mice, 22g, were divided into groups (n=4-5) and treated for 4 days with saline (5 mL/kg, i.p.) or CPT-11 (60 mg/kg, i.p.) or were given IL-18bp (IL-18 binding protein, 200 µg/animal/4 days, i.p.), aminoguanidine (AG, 50 mg/kg, s.c, 2x/day/4 days), IL-33 (1 µg/animal/4 days, i.v, 1h previously CPT-11) or loperamide ([4x3 mg/kg and 4x30 mg/kg]/animal, s.c.) co-administered with CPT-11. On day 5, diarrhea and leukocyte counts were assessed, and following sacrifice duodenal portions of the animal were collected to assess myeloperoxidase (MPO, neutrophils/mg tissue) and nitric oxide synthase (iNOS pM citruline /h/mg protein) activity, morphometric analysis, and in vitro contractility (%contraction in relation to KCl 60 mM). ANOVA/Newman Keuls or Kruskal Wallis/Dunn were used as statistical tests. P<0.05 was accepted. Results: IM was morphofunctionally similar in both BL and Swiss. CPT-11 induced leukopenia in all animal lineages despite the treatment given. Additionally, CPT-11 induced MPO, increased in vitro contractility and diarrheic events, and villi/crypt ratio reduction in BL and BC mice in comparison with saline treated mice (p<0.05). Despite the injection of CPT-11, Casp-1-/-, IL-18-/- or IL-18bp treated BC mice presented reduced MPO and iNOS activities and also increased villi/crypt ratio, reduced duodenal in vitro contractility and mild diarrhea similar to saline-treated mice (P>0.05), similar to the patterns seen in IL-33 treated mice. The reduced iNOS activity found in the earlier mice led us to investigate the CPT-11 effect on iNOS /- and aminoguanidine-treated mice. Lower IL-1beta and KC cytokine levels, as well as reduced MPO activity were found. Also, preserved villi/crypt ratio and muscle layer thickness, normal duodenal contractility and diarrheic events were seen similarly to saline-treated mice (p>0.05). However, CPT-11-treated PAFr-/- presented morphofunctional alterations and increased intestinal MPO activity. 5-LOX-/- or loperamide treated mice (positive control), both injected with CPT-11, exhibited increased MPO activity and morphometric alterations, but no exacerbation on in vitro contractility was observable. Conclusion: Taken together, these results suggest the role of caspase-1/Interleukin-18/nitric oxide cascade on pathogenesis of CPT-11-induced morphofunctional alteration and inflammatory response (IM). IL-33 seems to be a compensatory protective factor on this inflammatory cascade. 5-LOX and PAFr show a likely secondary role on IM pathophysiology.
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spelling Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitrícoMorphofunctional study and the inflammatory mediators involved on the pathogenesis of irinotecan (CPT-11)-induced intestinal mucositis in mice : role of caspase-1, interleukin-18 and nitric oxideMucositeFator de Ativação de PlaquetasMediadores da InflamaçãoInflamaçãoCitocinasSevere Intestinal Mucositis (IM) and diarrhea are frequent and dose-limiting side-effects of colon cancer chemotherapy with irinotecan (CPT-11). At present, much of IM pathophysiology remains unknown. Aims: To study the mechanisms and inflammatory mediators involved in CPT-11-induced intestinal mucositis, verifying the role of the protease caspase-1, cytokines interleukin-1 (IL-1), interleukin-18 (IL-18) and interleukin-33 (IL-33), nitric oxide, 5-lipoxygenase and PAF as well as the sequence of activation of these mediators in the inflammatory response and morphofunctional contexts. Methods: Swiss, C57BL/6 (BL), BALB/c (BC) male mice or caspases-1(Casp-1-/-), IL-18 (IL-18-/-), inducible nitric oxide synthase (iNOS-/-), 5-Lipoxygenase (5-LOX-/-), PAF receptor (PAFr-/-) knockout mice, 22g, were divided into groups (n=4-5) and treated for 4 days with saline (5 mL/kg, i.p.) or CPT-11 (60 mg/kg, i.p.) or were given IL-18bp (IL-18 binding protein, 200 µg/animal/4 days, i.p.), aminoguanidine (AG, 50 mg/kg, s.c, 2x/day/4 days), IL-33 (1 µg/animal/4 days, i.v, 1h previously CPT-11) or loperamide ([4x3 mg/kg and 4x30 mg/kg]/animal, s.c.) co-administered with CPT-11. On day 5, diarrhea and leukocyte counts were assessed, and following sacrifice duodenal portions of the animal were collected to assess myeloperoxidase (MPO, neutrophils/mg tissue) and nitric oxide synthase (iNOS pM citruline /h/mg protein) activity, morphometric analysis, and in vitro contractility (%contraction in relation to KCl 60 mM). ANOVA/Newman Keuls or Kruskal Wallis/Dunn were used as statistical tests. P<0.05 was accepted. Results: IM was morphofunctionally similar in both BL and Swiss. CPT-11 induced leukopenia in all animal lineages despite the treatment given. Additionally, CPT-11 induced MPO, increased in vitro contractility and diarrheic events, and villi/crypt ratio reduction in BL and BC mice in comparison with saline treated mice (p<0.05). Despite the injection of CPT-11, Casp-1-/-, IL-18-/- or IL-18bp treated BC mice presented reduced MPO and iNOS activities and also increased villi/crypt ratio, reduced duodenal in vitro contractility and mild diarrhea similar to saline-treated mice (P>0.05), similar to the patterns seen in IL-33 treated mice. The reduced iNOS activity found in the earlier mice led us to investigate the CPT-11 effect on iNOS /- and aminoguanidine-treated mice. Lower IL-1beta and KC cytokine levels, as well as reduced MPO activity were found. Also, preserved villi/crypt ratio and muscle layer thickness, normal duodenal contractility and diarrheic events were seen similarly to saline-treated mice (p>0.05). However, CPT-11-treated PAFr-/- presented morphofunctional alterations and increased intestinal MPO activity. 5-LOX-/- or loperamide treated mice (positive control), both injected with CPT-11, exhibited increased MPO activity and morphometric alterations, but no exacerbation on in vitro contractility was observable. Conclusion: Taken together, these results suggest the role of caspase-1/Interleukin-18/nitric oxide cascade on pathogenesis of CPT-11-induced morphofunctional alteration and inflammatory response (IM). IL-33 seems to be a compensatory protective factor on this inflammatory cascade. 5-LOX and PAFr show a likely secondary role on IM pathophysiology.A Mucosite Intestinal (MI) e a diarréia severas são efeitos colaterais freqüentes (15-25%) e limitantes da quimioterapia do câncer de cólon com Irinotecano (CPT-11). Até o presente momento, pouco se conhece sobre a fisiopatologia da MI. Objetivo: Estudar os mecanismos e mediadores envolvidos na mucosite intestinal induzida por CPT-11, verificando a participação da protease caspase-1, das citocinas interleucina-1 (IL-1), interleucina-18 (IL-18) e interleucina-33 (IL-33), do óxido nítrico, de mediadores 5-lipoxigenase e do PAF, assim como a seqüência de interação entre esses mediadores sob aspectos da resposta inflamatória e morfofuncionais. Métodos: Camundongos Swiss, C57BL/6 (BL), BALB/c (BC) ou knockout para Caspase-1 (Casp-/-), IL-18 (IL-18-/-), óxido nítrico sintase induzida (iNOS-/-), 5-Lipoxigenase (5-LOX-/-), receptor para PAF (PAFr-/-) machos, 22 g, foram divididos em grupos (n=4-5) e tratados por 4 dias com salina (5 mL/kg, i.p) ou CPT-11 (60 mg/kg, i.p) ou foram pré-tratados com IL-18bp (proteína ligante de IL18, 200 µg/animal/4 dias, i.p.), aminoguanidina (AG, 50 mg/kg, s.c, 2x/dia/4 dias), IL-33 (1 µg/animal/4 dias, i.v, 1h antes do CPT-11) ou loperamida ([4x3 mg/kg e 4x30 mg/kg]/animal, s.c.) em associação com CPT-11. No 5º dia, avaliou-se a diarréia por escores, o leucograma e, após sacrifício, coletou-se o duodeno para dosagem da atividade de mieloperoxidase (MPO, neutrófilos/mg tecido) e de óxido nítrico sintase induzida (iNOS pM citrulina/h/mg proteína), morfometria (razão vilo/cripta) e contratilidade in vitro à Acetilcolina (%contração em relação ao KCl60 mM). Para análise estatística utilizou-se ANOVA/Newman-Keuls ou Kruskal Wallis/Dunn. P<0,05 foi aceito. Resultados: Camundongos BL apresentaram um padrão de mucosite intestinal similar ao observado no camundongo Swiss sob aspectos morfofuncionais. O CPT-11 induziu leucopenia em todos os animais independentemente do tratamento aplicado. Adicionalmente, o CPT-11 induziu em animais BL e BC, não tratados, aumento de MPO intestinal, redução da relação vilo/cripta, amento da contratilidade in vitro e dos eventos de diarréia comparados com animais injetados somente com salina (p<0,05). A despeito da administração de CPT-11, animais Caspase-1-/-, IL-18-/- ou BC tratados com IL-18bp apresentaram atividade de MPO e de iNOS reduzidas, bem como aumento na relação vilo/cripta, menor contratilidade duodenal in vitro e eventos de diarréia atenuados em comparação aos respectivos controles tratados com salina (p>0,05). Dados idênticos também foram observados em camundongos injetados com IL-33 e que receberam CPT-11. A redução na atividade de iNOS naqueles animais motivou o estudo do efeito do CPT-11 em animais iNOS-/- ou tratados com aminoguanidina. Observaram-se menores níveis de citocinas IL-1beta e KC, bem como uma atividade de MPO reduzida no duodeno desses animais. Além disso, verificou-se preservação da relação vilo/cripta e da espessura da camada muscular, menor contratilidade duodenal in vitro e eventos de diarréia atenuados similar ao observado nos respectivos controles que receberam apenas salina (p>0,05). Porém, a administração de CPT-11 a animais PAFr-/- foi capaz de induzir alterações morfofuncionais e o aumento da atividade de MPO intestinal. Em camundongos 5-LOX /- ou tratados com loperamida (controle positivo), ambos os grupos injetados com CPT-11, observaram-se aumento da atividade de MPO e alterações morfométricas. Contudo, sobre aspectos funcionais de contratilidade in vitro, visualizou-se proteção. Conclusões: Esses resultados sugerem que a via caspase-1-IL-18 óxido nítrico contribui para o desenvolvimento da resposta inflamatória e alterações morfofuncionais na mucosite intestinal induzida por CPT-11. A IL-33 parece ser um fator protetor compensatório nessa cascata inflamatória. A 5-LOX e o PAFr parecem ter papel secundário na patogênese da mucosite intestinal por CPT-11.Ribeiro, Ronaldo de AlbuquerqueLima Júnior, Roberto César Pereira2012-10-01T16:30:37Z2012-10-01T16:30:37Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfLIMA JUNIOR, R. C. P. Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco. 200 f. Tese (Doutorado em Farmacologia) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2008.http://www.repositorio.ufc.br/handle/riufc/3843ark:/83112/001300000z1c8porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-06-29T13:54:11Zoai:repositorio.ufc.br:riufc/3843Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:20:22.767153Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
Morphofunctional study and the inflammatory mediators involved on the pathogenesis of irinotecan (CPT-11)-induced intestinal mucositis in mice : role of caspase-1, interleukin-18 and nitric oxide
title Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
spellingShingle Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
Lima Júnior, Roberto César Pereira
Mucosite
Fator de Ativação de Plaquetas
Mediadores da Inflamação
Inflamação
Citocinas
title_short Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
title_full Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
title_fullStr Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
title_full_unstemmed Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
title_sort Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco
author Lima Júnior, Roberto César Pereira
author_facet Lima Júnior, Roberto César Pereira
author_role author
dc.contributor.none.fl_str_mv Ribeiro, Ronaldo de Albuquerque
dc.contributor.author.fl_str_mv Lima Júnior, Roberto César Pereira
dc.subject.por.fl_str_mv Mucosite
Fator de Ativação de Plaquetas
Mediadores da Inflamação
Inflamação
Citocinas
topic Mucosite
Fator de Ativação de Plaquetas
Mediadores da Inflamação
Inflamação
Citocinas
description Severe Intestinal Mucositis (IM) and diarrhea are frequent and dose-limiting side-effects of colon cancer chemotherapy with irinotecan (CPT-11). At present, much of IM pathophysiology remains unknown. Aims: To study the mechanisms and inflammatory mediators involved in CPT-11-induced intestinal mucositis, verifying the role of the protease caspase-1, cytokines interleukin-1 (IL-1), interleukin-18 (IL-18) and interleukin-33 (IL-33), nitric oxide, 5-lipoxygenase and PAF as well as the sequence of activation of these mediators in the inflammatory response and morphofunctional contexts. Methods: Swiss, C57BL/6 (BL), BALB/c (BC) male mice or caspases-1(Casp-1-/-), IL-18 (IL-18-/-), inducible nitric oxide synthase (iNOS-/-), 5-Lipoxygenase (5-LOX-/-), PAF receptor (PAFr-/-) knockout mice, 22g, were divided into groups (n=4-5) and treated for 4 days with saline (5 mL/kg, i.p.) or CPT-11 (60 mg/kg, i.p.) or were given IL-18bp (IL-18 binding protein, 200 µg/animal/4 days, i.p.), aminoguanidine (AG, 50 mg/kg, s.c, 2x/day/4 days), IL-33 (1 µg/animal/4 days, i.v, 1h previously CPT-11) or loperamide ([4x3 mg/kg and 4x30 mg/kg]/animal, s.c.) co-administered with CPT-11. On day 5, diarrhea and leukocyte counts were assessed, and following sacrifice duodenal portions of the animal were collected to assess myeloperoxidase (MPO, neutrophils/mg tissue) and nitric oxide synthase (iNOS pM citruline /h/mg protein) activity, morphometric analysis, and in vitro contractility (%contraction in relation to KCl 60 mM). ANOVA/Newman Keuls or Kruskal Wallis/Dunn were used as statistical tests. P<0.05 was accepted. Results: IM was morphofunctionally similar in both BL and Swiss. CPT-11 induced leukopenia in all animal lineages despite the treatment given. Additionally, CPT-11 induced MPO, increased in vitro contractility and diarrheic events, and villi/crypt ratio reduction in BL and BC mice in comparison with saline treated mice (p<0.05). Despite the injection of CPT-11, Casp-1-/-, IL-18-/- or IL-18bp treated BC mice presented reduced MPO and iNOS activities and also increased villi/crypt ratio, reduced duodenal in vitro contractility and mild diarrhea similar to saline-treated mice (P>0.05), similar to the patterns seen in IL-33 treated mice. The reduced iNOS activity found in the earlier mice led us to investigate the CPT-11 effect on iNOS /- and aminoguanidine-treated mice. Lower IL-1beta and KC cytokine levels, as well as reduced MPO activity were found. Also, preserved villi/crypt ratio and muscle layer thickness, normal duodenal contractility and diarrheic events were seen similarly to saline-treated mice (p>0.05). However, CPT-11-treated PAFr-/- presented morphofunctional alterations and increased intestinal MPO activity. 5-LOX-/- or loperamide treated mice (positive control), both injected with CPT-11, exhibited increased MPO activity and morphometric alterations, but no exacerbation on in vitro contractility was observable. Conclusion: Taken together, these results suggest the role of caspase-1/Interleukin-18/nitric oxide cascade on pathogenesis of CPT-11-induced morphofunctional alteration and inflammatory response (IM). IL-33 seems to be a compensatory protective factor on this inflammatory cascade. 5-LOX and PAFr show a likely secondary role on IM pathophysiology.
publishDate 2008
dc.date.none.fl_str_mv 2008
2012-10-01T16:30:37Z
2012-10-01T16:30:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.uri.fl_str_mv LIMA JUNIOR, R. C. P. Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco. 200 f. Tese (Doutorado em Farmacologia) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2008.
http://www.repositorio.ufc.br/handle/riufc/3843
dc.identifier.dark.fl_str_mv ark:/83112/001300000z1c8
identifier_str_mv LIMA JUNIOR, R. C. P. Estudo morfofuncional e dos mediadores inflamatórios envolvidos na patogênese da mucosite intestinal induzida por irinotecano (CPT-11) em camundongos : papel da caspase-1, interleucina-18 e óxido nitríco. 200 f. Tese (Doutorado em Farmacologia) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2008.
ark:/83112/001300000z1c8
url http://www.repositorio.ufc.br/handle/riufc/3843
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