Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus

Detalhes bibliográficos
Autor(a) principal: Kerkis, Alexandre
Data de Publicação: 2004
Outros Autores: Kerkis, Irina, Rádis-Baptista, Gandhi, Oliveira, Eduardo B., Vianna-Morgante, Angela M., Pereira, Lygia V., Yamane, Tetsuo
Tipo de documento: Artigo de conferência
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/59812
Resumo: Herein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed in both fixed and unfixed cells. In the cytoplasm, crotamine specifically associates with centrosomes and thus allows us to follow the process of centriole duplication and separation. In the nucleus, it binds to the chromosomes at S/G2 phase, when centrioles start dividing. Moreover, crotamine appears as a marker of actively proliferating cells, as shown by 5-BrdU cell-proliferation assay. Crotamine in the micromolar range proved nontoxic to any of the cell cultures tested and did not affect the pluripotency of ES cells or the development of mouse embryos
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spelling Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificusCrotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificusCromossomasDnaPeptídeosHerein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed in both fixed and unfixed cells. In the cytoplasm, crotamine specifically associates with centrosomes and thus allows us to follow the process of centriole duplication and separation. In the nucleus, it binds to the chromosomes at S/G2 phase, when centrioles start dividing. Moreover, crotamine appears as a marker of actively proliferating cells, as shown by 5-BrdU cell-proliferation assay. Crotamine in the micromolar range proved nontoxic to any of the cell cultures tested and did not affect the pluripotency of ES cells or the development of mouse embryosThe FASEB Journal express2021-08-02T17:18:59Z2021-08-02T17:18:59Z2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectapplication/pdfKERKIS, Alexandre; KERKIS, Irina; RÁDIS-BAPTISTA, Ghandi; OLIVEIRA, Eduardo B.; VIANNA-MORGANTE, Angela M.; PEREIRA, Lygia V.; YAMANE, Tetsuo. Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus The FASEB Journal express, United States, art. 10.1096/fj.03-1459fje. 2004.0892-6638http://www.repositorio.ufc.br/handle/riufc/59812Kerkis, AlexandreKerkis, IrinaRádis-Baptista, GandhiOliveira, Eduardo B.Vianna-Morgante, Angela M.Pereira, Lygia V.Yamane, Tetsuoporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-11-30T14:22:03Zoai:repositorio.ufc.br:riufc/59812Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:43:31.880050Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
title Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
spellingShingle Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
Kerkis, Alexandre
Cromossomas
Dna
Peptídeos
title_short Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
title_full Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
title_fullStr Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
title_full_unstemmed Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
title_sort Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
author Kerkis, Alexandre
author_facet Kerkis, Alexandre
Kerkis, Irina
Rádis-Baptista, Gandhi
Oliveira, Eduardo B.
Vianna-Morgante, Angela M.
Pereira, Lygia V.
Yamane, Tetsuo
author_role author
author2 Kerkis, Irina
Rádis-Baptista, Gandhi
Oliveira, Eduardo B.
Vianna-Morgante, Angela M.
Pereira, Lygia V.
Yamane, Tetsuo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kerkis, Alexandre
Kerkis, Irina
Rádis-Baptista, Gandhi
Oliveira, Eduardo B.
Vianna-Morgante, Angela M.
Pereira, Lygia V.
Yamane, Tetsuo
dc.subject.por.fl_str_mv Cromossomas
Dna
Peptídeos
topic Cromossomas
Dna
Peptídeos
description Herein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed in both fixed and unfixed cells. In the cytoplasm, crotamine specifically associates with centrosomes and thus allows us to follow the process of centriole duplication and separation. In the nucleus, it binds to the chromosomes at S/G2 phase, when centrioles start dividing. Moreover, crotamine appears as a marker of actively proliferating cells, as shown by 5-BrdU cell-proliferation assay. Crotamine in the micromolar range proved nontoxic to any of the cell cultures tested and did not affect the pluripotency of ES cells or the development of mouse embryos
publishDate 2004
dc.date.none.fl_str_mv 2004
2021-08-02T17:18:59Z
2021-08-02T17:18:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv KERKIS, Alexandre; KERKIS, Irina; RÁDIS-BAPTISTA, Ghandi; OLIVEIRA, Eduardo B.; VIANNA-MORGANTE, Angela M.; PEREIRA, Lygia V.; YAMANE, Tetsuo. Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus The FASEB Journal express, United States, art. 10.1096/fj.03-1459fje. 2004.
0892-6638
http://www.repositorio.ufc.br/handle/riufc/59812
identifier_str_mv KERKIS, Alexandre; KERKIS, Irina; RÁDIS-BAPTISTA, Ghandi; OLIVEIRA, Eduardo B.; VIANNA-MORGANTE, Angela M.; PEREIRA, Lygia V.; YAMANE, Tetsuo. Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus The FASEB Journal express, United States, art. 10.1096/fj.03-1459fje. 2004.
0892-6638
url http://www.repositorio.ufc.br/handle/riufc/59812
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The FASEB Journal express
publisher.none.fl_str_mv The FASEB Journal express
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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