Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/59812 |
Resumo: | Herein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed in both fixed and unfixed cells. In the cytoplasm, crotamine specifically associates with centrosomes and thus allows us to follow the process of centriole duplication and separation. In the nucleus, it binds to the chromosomes at S/G2 phase, when centrioles start dividing. Moreover, crotamine appears as a marker of actively proliferating cells, as shown by 5-BrdU cell-proliferation assay. Crotamine in the micromolar range proved nontoxic to any of the cell cultures tested and did not affect the pluripotency of ES cells or the development of mouse embryos |
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Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificusCrotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificusCromossomasDnaPeptídeosHerein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed in both fixed and unfixed cells. In the cytoplasm, crotamine specifically associates with centrosomes and thus allows us to follow the process of centriole duplication and separation. In the nucleus, it binds to the chromosomes at S/G2 phase, when centrioles start dividing. Moreover, crotamine appears as a marker of actively proliferating cells, as shown by 5-BrdU cell-proliferation assay. Crotamine in the micromolar range proved nontoxic to any of the cell cultures tested and did not affect the pluripotency of ES cells or the development of mouse embryosThe FASEB Journal express2021-08-02T17:18:59Z2021-08-02T17:18:59Z2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectapplication/pdfKERKIS, Alexandre; KERKIS, Irina; RÁDIS-BAPTISTA, Ghandi; OLIVEIRA, Eduardo B.; VIANNA-MORGANTE, Angela M.; PEREIRA, Lygia V.; YAMANE, Tetsuo. Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus The FASEB Journal express, United States, art. 10.1096/fj.03-1459fje. 2004.0892-6638http://www.repositorio.ufc.br/handle/riufc/59812Kerkis, AlexandreKerkis, IrinaRádis-Baptista, GandhiOliveira, Eduardo B.Vianna-Morgante, Angela M.Pereira, Lygia V.Yamane, Tetsuoporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-11-30T14:22:03Zoai:repositorio.ufc.br:riufc/59812Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:43:31.880050Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus |
title |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus |
spellingShingle |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus Kerkis, Alexandre Cromossomas Dna Peptídeos |
title_short |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus |
title_full |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus |
title_fullStr |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus |
title_full_unstemmed |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus |
title_sort |
Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus |
author |
Kerkis, Alexandre |
author_facet |
Kerkis, Alexandre Kerkis, Irina Rádis-Baptista, Gandhi Oliveira, Eduardo B. Vianna-Morgante, Angela M. Pereira, Lygia V. Yamane, Tetsuo |
author_role |
author |
author2 |
Kerkis, Irina Rádis-Baptista, Gandhi Oliveira, Eduardo B. Vianna-Morgante, Angela M. Pereira, Lygia V. Yamane, Tetsuo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Kerkis, Alexandre Kerkis, Irina Rádis-Baptista, Gandhi Oliveira, Eduardo B. Vianna-Morgante, Angela M. Pereira, Lygia V. Yamane, Tetsuo |
dc.subject.por.fl_str_mv |
Cromossomas Dna Peptídeos |
topic |
Cromossomas Dna Peptídeos |
description |
Herein we report that crotamine, a small lysine- and cysteine-rich protein from the venom of the South American rattlesnake, can rapidly penetrate into different cell types and mouse blastocysts in vitro. In vivo, crotamine strongly labels cells from mouse bone marrow and spleen and from peritoneal liquid, as shown by fluorescent confocal laser-scanning microscopy. Nuclear localization of crotamine was observed in both fixed and unfixed cells. In the cytoplasm, crotamine specifically associates with centrosomes and thus allows us to follow the process of centriole duplication and separation. In the nucleus, it binds to the chromosomes at S/G2 phase, when centrioles start dividing. Moreover, crotamine appears as a marker of actively proliferating cells, as shown by 5-BrdU cell-proliferation assay. Crotamine in the micromolar range proved nontoxic to any of the cell cultures tested and did not affect the pluripotency of ES cells or the development of mouse embryos |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2021-08-02T17:18:59Z 2021-08-02T17:18:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
KERKIS, Alexandre; KERKIS, Irina; RÁDIS-BAPTISTA, Ghandi; OLIVEIRA, Eduardo B.; VIANNA-MORGANTE, Angela M.; PEREIRA, Lygia V.; YAMANE, Tetsuo. Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus The FASEB Journal express, United States, art. 10.1096/fj.03-1459fje. 2004. 0892-6638 http://www.repositorio.ufc.br/handle/riufc/59812 |
identifier_str_mv |
KERKIS, Alexandre; KERKIS, Irina; RÁDIS-BAPTISTA, Ghandi; OLIVEIRA, Eduardo B.; VIANNA-MORGANTE, Angela M.; PEREIRA, Lygia V.; YAMANE, Tetsuo. Crotamine is a novel cell-penetrating protein from the venom of rattlesnake Crotalus durissus terrificus The FASEB Journal express, United States, art. 10.1096/fj.03-1459fje. 2004. 0892-6638 |
url |
http://www.repositorio.ufc.br/handle/riufc/59812 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
The FASEB Journal express |
publisher.none.fl_str_mv |
The FASEB Journal express |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1813028921425264640 |