Tolerância ao estresse hídrico em diferentes variedades de feijão-caupi em fase de plântula

• Autor(a) principal: Fiege, Leonardo Barros da Costa
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/30990
• topic: Vigna unguiculata; Tolerância a seca; Caracterização genética; Fenotipagem

Cowpea production has a high socioeconomic importance in the northeastern region of Brazil, where most of its production is located, but this region is characterized by the occurrence of water shortage which causes a considerable decrease of the production in this species. therefore, it is important to identify and develop varieties of cowpea adapted to the dry climate. The objective of this work was to characterize different varieties of cowpea in seedling stage, through the use of ISSR markers and the Screening Box method, to identify variates that may have drought tolerance. The evaluation by means of ISSR markers was performed in the laboratory and consisted of the analysis of 50 DNA samples extracted within the different varieties of cowpea evaluated. This analysis sought to identify genetic variability for drought tolerance utilizing a multivariate and cluster analysis. The evaluation through the Screening Box method occurred during the months of September to October of 2017, in a greenhouse located at the Universidade Federal do Ceará (UFC). In this experiment, water stress was induced in plants of 22 varieties seven days after transplant. These varieties were selected based on the genetic characterization data. In both experiments local varieties acquired in several cities of the state of Ceará were evaluated. The control varieties in the two experiments were Pingo de Ouro 1,2 (tolerant to water stress) and Santo Inácio (sensitive to water stress). In this first experiment genetic variability was identified among the analyzed varieties, observing the formation of six groups. Several varieties (44) were grouped with the control tolerant strain, which indicates genetic proximity. In the greenhouse experiment, the statistical analysis indicated a difference between the treatments. The varieties of code CCE-010, CCE-012, CCE-012, CCE-012, CCE-010, CCE-012, CCE-012 and CCE-012 can be considered tolerant to water stress by the results of the experiment.