Antifungal resistance and virulence among Candida spp. from captive amazonian manatees and west Indian manatees : potential impacts on animal and environmental health

• Autor(a) principal: Sidrim, José Júlio Costa
• Data de Publicação: 2016
• Outros Autores: Carvalho, Vitor Luz, Castelo-Branco, Débora de Souza Collares Maia, Brilhante, Raimunda Sâmia Nogueira, Guedes, Gláucia Morgana de Melo, Barbosa, Giovanna Riello, Lazzarini, Stella Maris, Oliveira, Daniella Carvalho Ribeiro, Meirelles, Ana Carolina Oliveira de, Attademo, Fernanda Löffler Niemeyer, Freire, Augusto Carlos da Bôaviagem, Pereira-Neto, Pereira-Neto, Cordeiro, Rossana de Aguiar, Moreira, José Luciano Bezerra, Rocha, Marcos Fábio Gadelha
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/15960
• topic: Trichechus; Saúde Ambiental

This work aimed at evaluating the antifungal susceptibility and production of virulence factors by Candida spp. isolated from sirenians in Brazil. The isolates (n = 105) were recovered from the natural cavities of Amazonian and West Indian manatees and were tested for the susceptibility to amphotericin B, itraconazole, and fluconazole and for the production of phospholipases, proteases, and biofilm. The minimum inhibitory concentrations (MICs) for amphotericin B ranged from 0.03 to 1 lg/mL, and no resistant isolates were detected. Itraconazole and fluconazole MICs ranged from 0.03 to 16 lg/mL and from 0.125 to 64 lg/mL, respectively, and 35.2% (37/105) of the isolates were resistant to at least one of these azole drugs. Concerning the production of virulence factors, phospholipase activity was observed in 67.6% (71/105) of the isolates, while protease activity and biofilm production were detected in 50.5% (53/105) and 32.4% (34/105) of the isolates, respectively. Since the natural cavities of manatees are colonized by resistant and virulent strains of Candida spp., these animals can act as sources of resistance and virulence genes for the environment, conspecifics and other animal species, demonstrating the potential environmental impacts associated with their release back into their natural habitat.