Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/23927 |
Resumo: | The leafhopper (Liriomyza sativae) today is considered one of the main plagues of melon in Brazil, which has been stimulating the abusive use of insecticides to overcome the problems caused by the pest. In this sense, the use of resistant cultivars is a promising alternative. Thus, the present study aimed to evaluate the resistance of melon genotypes in relation to the fruit fly. For that, 21 genotypes of melon were evaluated, from the Genetic Improvement Program of Meloeiro of Embrapa, and the commercial hybrid "Goldex" testifies. The non-preference for oviposition and feeding were evaluated in tests with and without a choice; The effect of genotype antibiosis through larval and pupal viability; As well as the number of trichomes and volatile compounds present in the leaves. For evaluation, the following genotypes AC 35, AC 43, 341, 343, 333 and the Goldex hybrid were selected for the morphological characterization of leaves (adaxial and abaxial) and release of volatile compounds. Quantification of trichomes was carried out in Scanning Electron Microscope (SEM), where the number of trichomes was quantified and correlated with oviposition and plague feeding. The volatile analyzes in the melon leaves were carried out using the solid phase microextraction technique coupled to gas chromatography with mass spectrometry (SPME-GC-MS). As for the non-preference for oviposition (number of oviposition punctures) and feeding (number of feeding punctures), there was no difference between the genotypes and the Goldex control, in a test with a chance of choice. However, in the confinement test, genotypes 343 and AC 43 were the least oviposited by L. sativae, with mean values of 0.9 and 1.3 respectively; While genotype 343 was the least preferred for feeding, with 0.7 feeding punches / 4cm in diameter. As for larval and pupal viability, nine genotypes were more promising: 339, 346, 343, 341, 334, 331, 333, 313 and 330, and a possible antibiosis effect was found in these treatments. In relation to the density of trichomes, there was no difference between the treatments and no correlation was found between the density (adaxial and abaxial) and the number of oviposition and feeding punctures. For the volatile analysis of the leaves, a total of ten volatile compounds distributed among the treatments were obtained. From this study it is suggested to have antibiosis resistance in the nine genotypes mentioned above and the presence of some degree of antixenosis in genotypes 343 and AC43, but the trichomes and volatile compounds identified in the melon genotypes do not present any action Antioxenotic for oviposition and feeding of L. sativae. |
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Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae)Resistance of melon genotypes to leafminer, Liriomyza sativae Blanchard (Diptera: Agromyzidae)Cucumis meloNão-preferênciaAntibioseTricomasVoláteisThe leafhopper (Liriomyza sativae) today is considered one of the main plagues of melon in Brazil, which has been stimulating the abusive use of insecticides to overcome the problems caused by the pest. In this sense, the use of resistant cultivars is a promising alternative. Thus, the present study aimed to evaluate the resistance of melon genotypes in relation to the fruit fly. For that, 21 genotypes of melon were evaluated, from the Genetic Improvement Program of Meloeiro of Embrapa, and the commercial hybrid "Goldex" testifies. The non-preference for oviposition and feeding were evaluated in tests with and without a choice; The effect of genotype antibiosis through larval and pupal viability; As well as the number of trichomes and volatile compounds present in the leaves. For evaluation, the following genotypes AC 35, AC 43, 341, 343, 333 and the Goldex hybrid were selected for the morphological characterization of leaves (adaxial and abaxial) and release of volatile compounds. Quantification of trichomes was carried out in Scanning Electron Microscope (SEM), where the number of trichomes was quantified and correlated with oviposition and plague feeding. The volatile analyzes in the melon leaves were carried out using the solid phase microextraction technique coupled to gas chromatography with mass spectrometry (SPME-GC-MS). As for the non-preference for oviposition (number of oviposition punctures) and feeding (number of feeding punctures), there was no difference between the genotypes and the Goldex control, in a test with a chance of choice. However, in the confinement test, genotypes 343 and AC 43 were the least oviposited by L. sativae, with mean values of 0.9 and 1.3 respectively; While genotype 343 was the least preferred for feeding, with 0.7 feeding punches / 4cm in diameter. As for larval and pupal viability, nine genotypes were more promising: 339, 346, 343, 341, 334, 331, 333, 313 and 330, and a possible antibiosis effect was found in these treatments. In relation to the density of trichomes, there was no difference between the treatments and no correlation was found between the density (adaxial and abaxial) and the number of oviposition and feeding punctures. For the volatile analysis of the leaves, a total of ten volatile compounds distributed among the treatments were obtained. From this study it is suggested to have antibiosis resistance in the nine genotypes mentioned above and the presence of some degree of antixenosis in genotypes 343 and AC43, but the trichomes and volatile compounds identified in the melon genotypes do not present any action Antioxenotic for oviposition and feeding of L. sativae.A mosca-minadora (Liriomyza sativae) hoje é considerada uma das principais pragas do meloeiro no Brasil, o que vêm estimulando o uso abusivo de inseticidas para contornar os problemas ocasionados pela praga. Neste sentido, o uso de cultivares resistente é uma alternativa promissora. Assim, a presente pesquisa objetivou avaliar a resistência de genótipos de meloeiro em relação a mosca-minadora. Para tanto, avaliou-se 21 genótipos de meloeiro, provenientes do Programa de Melhoramento Genético de Meloeiro da Embrapa, e o híbrido comercial “Goldex” testemunha. Avaliaram-se a não-preferência por oviposição e alimentação, em testes com e sem chance de escolha; o efeito de antibiose dos genótipos através da viabilidade larval e pupal; assim como número de tricomas e compostos voláteis presentes nas folhas. Para avaliação, foram selecionados os seguintes genótipos AC 35, AC 43, 341, 343, 333 e o hibrido Goldex, para caracterização morfológica das folhas (adaxial e abaxial) e liberação de compostos voláteis. Realizou-se a quantificação de tricomas em Microscópio Eletrônico de Varredura (MEV), onde o número de tricomas foi quantificado e correlacionado com a oviposição e alimentação da praga. As análises de voláteis nas folhas de meloeiro foram realizadas utilizando-se da técnica da microextração em fase sólida acoplada a cromatografia gasosa com espectrometria de massa (SPME-GC-MS). Quanto à não-preferência para oviposição (número de puncturas de oviposição) e alimentação (número de puncturas de alimentação), não houve diferença entre os genótipos e a testemunha Goldex, em teste com chance de escolha. Porém, no teste em confinamento, os genótipos 343 e AC 43 foram os menos ovipositados por L. sativae, com valores médios de 0,9 e 1,3 respectivamente; enquanto, o genótipo 343 foi o menos preferido para alimentação, com 0,7 puncturas de alimentação/4cm de diâmetro. Quanto a viabilidade larval e pupal, nove genótipos mostraram-se mais promissores: 339, 346, 343, 341, 334, 331, 333, 313 e 330, sendo encontrado um possível efeito de antibiose nesses tratamentos. Em relação à densidade de tricomas, não houve diferença entre os tratamentos e não foi encontrado correlação entre a densidade (adaxial e abaxial) e o número de puncturas de oviposição e alimentação. Para a análise de voláteis das folhas, obteve-se um total de dez compostos voláteis distribuídos entre os tratamentos. A partir deste estudo sugerese haver resistência, do tipo antibiose nos nove genótipos, citados acima, e a presença de algum grau de antixenose nos genótipos 343 e AC43, porém os tricomas e os compostos voláteis identificados nos genótipos de meloeiro, não apresentam ação antixenótica para oviposição e alimentação de L. sativae.Dias-Pini, Nivia da SilvaOliveira, Josielma Monteiro de2017-07-11T11:55:26Z2017-07-11T11:55:26Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfOLIVEIRA, J. M. de. Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae). 2017. 55f. Dissertação (Mestrado em Agronomia/Fitotecnia)- Universidade Federal do Ceará, Fortaleza, 2017.http://www.repositorio.ufc.br/handle/riufc/23927porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-08-04T18:44:02Zoai:repositorio.ufc.br:riufc/23927Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:51:56.449969Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) Resistance of melon genotypes to leafminer, Liriomyza sativae Blanchard (Diptera: Agromyzidae) |
title |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) |
spellingShingle |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) Oliveira, Josielma Monteiro de Cucumis melo Não-preferência Antibiose Tricomas Voláteis |
title_short |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) |
title_full |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) |
title_fullStr |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) |
title_full_unstemmed |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) |
title_sort |
Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae) |
author |
Oliveira, Josielma Monteiro de |
author_facet |
Oliveira, Josielma Monteiro de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Dias-Pini, Nivia da Silva |
dc.contributor.author.fl_str_mv |
Oliveira, Josielma Monteiro de |
dc.subject.por.fl_str_mv |
Cucumis melo Não-preferência Antibiose Tricomas Voláteis |
topic |
Cucumis melo Não-preferência Antibiose Tricomas Voláteis |
description |
The leafhopper (Liriomyza sativae) today is considered one of the main plagues of melon in Brazil, which has been stimulating the abusive use of insecticides to overcome the problems caused by the pest. In this sense, the use of resistant cultivars is a promising alternative. Thus, the present study aimed to evaluate the resistance of melon genotypes in relation to the fruit fly. For that, 21 genotypes of melon were evaluated, from the Genetic Improvement Program of Meloeiro of Embrapa, and the commercial hybrid "Goldex" testifies. The non-preference for oviposition and feeding were evaluated in tests with and without a choice; The effect of genotype antibiosis through larval and pupal viability; As well as the number of trichomes and volatile compounds present in the leaves. For evaluation, the following genotypes AC 35, AC 43, 341, 343, 333 and the Goldex hybrid were selected for the morphological characterization of leaves (adaxial and abaxial) and release of volatile compounds. Quantification of trichomes was carried out in Scanning Electron Microscope (SEM), where the number of trichomes was quantified and correlated with oviposition and plague feeding. The volatile analyzes in the melon leaves were carried out using the solid phase microextraction technique coupled to gas chromatography with mass spectrometry (SPME-GC-MS). As for the non-preference for oviposition (number of oviposition punctures) and feeding (number of feeding punctures), there was no difference between the genotypes and the Goldex control, in a test with a chance of choice. However, in the confinement test, genotypes 343 and AC 43 were the least oviposited by L. sativae, with mean values of 0.9 and 1.3 respectively; While genotype 343 was the least preferred for feeding, with 0.7 feeding punches / 4cm in diameter. As for larval and pupal viability, nine genotypes were more promising: 339, 346, 343, 341, 334, 331, 333, 313 and 330, and a possible antibiosis effect was found in these treatments. In relation to the density of trichomes, there was no difference between the treatments and no correlation was found between the density (adaxial and abaxial) and the number of oviposition and feeding punctures. For the volatile analysis of the leaves, a total of ten volatile compounds distributed among the treatments were obtained. From this study it is suggested to have antibiosis resistance in the nine genotypes mentioned above and the presence of some degree of antixenosis in genotypes 343 and AC43, but the trichomes and volatile compounds identified in the melon genotypes do not present any action Antioxenotic for oviposition and feeding of L. sativae. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-11T11:55:26Z 2017-07-11T11:55:26Z 2017 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA, J. M. de. Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae). 2017. 55f. Dissertação (Mestrado em Agronomia/Fitotecnia)- Universidade Federal do Ceará, Fortaleza, 2017. http://www.repositorio.ufc.br/handle/riufc/23927 |
identifier_str_mv |
OLIVEIRA, J. M. de. Resistência de genótipos de meloeiro à mosca-minadora, Liriomyza sativae Blanchard (Diptera: Agromyzidae). 2017. 55f. Dissertação (Mestrado em Agronomia/Fitotecnia)- Universidade Federal do Ceará, Fortaleza, 2017. |
url |
http://www.repositorio.ufc.br/handle/riufc/23927 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
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UFC |
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UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
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