Human IgG adsorption by affinity chromatography using FPLC Contichrom

Detalhes bibliográficos
Autor(a) principal: Santos, Santangela Oliveira
Data de Publicação: 2017
Outros Autores: Gondim, Diego Romão, Azevedo, Diana Cristina Silva de, Silva Júnior, Ivanildo José da
Tipo de documento: Artigo de conferência
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/54465
Resumo: Purification of human IgG by dye-ligand affinity chromatography was investigated. Procion Red and Reactive Red 120 dyes were immobilized on epoxide chitosan/alginate. Chromatographic experiments were conducted by using binary solution (IgG and human serum albumin-HSA) and human serum solution. The results showed that IgG was preferentially retained in both chromatographic matrices according to the electrophoresis analyze.
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network_name_str Repositório Institucional da Universidade Federal do Ceará (UFC)
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spelling Human IgG adsorption by affinity chromatography using FPLC ContichromHuman IgG adsorption by affinity chromatography using FPLC ContichromImunoglobulina GCromatografiaAdsorçãoPurification of human IgG by dye-ligand affinity chromatography was investigated. Procion Red and Reactive Red 120 dyes were immobilized on epoxide chitosan/alginate. Chromatographic experiments were conducted by using binary solution (IgG and human serum albumin-HSA) and human serum solution. The results showed that IgG was preferentially retained in both chromatographic matrices according to the electrophoresis analyze.2020-10-06T13:23:50Z2020-10-06T13:23:50Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectapplication/pdfSANTOS, Santangela Oliveira; GONDIM, Diego Romão; AZEVEDO, Diana Cristina Silva de; SILVA JUNIOR, Ivanildo José da. Human IgG adsorption by affinity chromatography using FPLC Contichrom. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, XXI; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, XII., 3 a 6 de set. 2017, Aracaju, Sergipe, Brasil. Anais [...] Aracaju, Sergipe, 2017.2447 2816http://www.repositorio.ufc.br/handle/riufc/54465Santos, Santangela OliveiraGondim, Diego RomãoAzevedo, Diana Cristina Silva deSilva Júnior, Ivanildo José daporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-06-08T16:31:03Zoai:repositorio.ufc.br:riufc/54465Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:22:44.188630Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Human IgG adsorption by affinity chromatography using FPLC Contichrom
Human IgG adsorption by affinity chromatography using FPLC Contichrom
title Human IgG adsorption by affinity chromatography using FPLC Contichrom
spellingShingle Human IgG adsorption by affinity chromatography using FPLC Contichrom
Santos, Santangela Oliveira
Imunoglobulina G
Cromatografia
Adsorção
title_short Human IgG adsorption by affinity chromatography using FPLC Contichrom
title_full Human IgG adsorption by affinity chromatography using FPLC Contichrom
title_fullStr Human IgG adsorption by affinity chromatography using FPLC Contichrom
title_full_unstemmed Human IgG adsorption by affinity chromatography using FPLC Contichrom
title_sort Human IgG adsorption by affinity chromatography using FPLC Contichrom
author Santos, Santangela Oliveira
author_facet Santos, Santangela Oliveira
Gondim, Diego Romão
Azevedo, Diana Cristina Silva de
Silva Júnior, Ivanildo José da
author_role author
author2 Gondim, Diego Romão
Azevedo, Diana Cristina Silva de
Silva Júnior, Ivanildo José da
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos, Santangela Oliveira
Gondim, Diego Romão
Azevedo, Diana Cristina Silva de
Silva Júnior, Ivanildo José da
dc.subject.por.fl_str_mv Imunoglobulina G
Cromatografia
Adsorção
topic Imunoglobulina G
Cromatografia
Adsorção
description Purification of human IgG by dye-ligand affinity chromatography was investigated. Procion Red and Reactive Red 120 dyes were immobilized on epoxide chitosan/alginate. Chromatographic experiments were conducted by using binary solution (IgG and human serum albumin-HSA) and human serum solution. The results showed that IgG was preferentially retained in both chromatographic matrices according to the electrophoresis analyze.
publishDate 2017
dc.date.none.fl_str_mv 2017
2020-10-06T13:23:50Z
2020-10-06T13:23:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv SANTOS, Santangela Oliveira; GONDIM, Diego Romão; AZEVEDO, Diana Cristina Silva de; SILVA JUNIOR, Ivanildo José da. Human IgG adsorption by affinity chromatography using FPLC Contichrom. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, XXI; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, XII., 3 a 6 de set. 2017, Aracaju, Sergipe, Brasil. Anais [...] Aracaju, Sergipe, 2017.
2447 2816
http://www.repositorio.ufc.br/handle/riufc/54465
identifier_str_mv SANTOS, Santangela Oliveira; GONDIM, Diego Romão; AZEVEDO, Diana Cristina Silva de; SILVA JUNIOR, Ivanildo José da. Human IgG adsorption by affinity chromatography using FPLC Contichrom. In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, XXI; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, XII., 3 a 6 de set. 2017, Aracaju, Sergipe, Brasil. Anais [...] Aracaju, Sergipe, 2017.
2447 2816
url http://www.repositorio.ufc.br/handle/riufc/54465
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1813028778708828160

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