Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/18722 |
Resumo: | Himatanthus drasticus (Apocynaceae) is traditionally used in folk medicine in Northeastern Brazil. The plant is wildly distribuited from Guianas until Southeast Brazil. Latex obtained by cutting its stem bark is mixed with water and extensively sold in public markets. People use Janaguba milk (“Leite de Janaguba”- LJ) to treat or prevent different inflammatory disorders such as gastritis, ulcers as well as cancer, among other uses. Despite, there is not enough scientific data confirming these pharmacological properties. In this work, Janaguba milk was preliminarily investigated in an attempt to validate its anti- inflammatory activity. Its major protein fraction (HdLP) was assessed for the presence of anti- inflammatory, anti-nociceptive and toxicological effects. LJ inhibited the inflammatory response induced by carrageenan in rats (98%, p < 0.05), whereas HdLP did it independent of the route of administration. HdLP significantly reduced the infiltration of neutrophils into the peritoneal cavity (96%, p < 0.05) concomitant with increased nitric oxide synthesis in plasma and decrease of pro-inflammatory cytokines IL- 1 and TNF-α in peritoneal fluid. This fraction also prevented vacuolization and Kupfer cell hyperplasia caused by carrageenan in liver. Further, the anti-inflammatory properties were shown to be associated with the protein fraction of LJ. HdLP exhibited anti-inflammation, even after heat-treatment (100 °C, 30 min) or proteolysis. Moreover, a pro-inflammatory effect was observed after HdLP treatment. It also suppressed abdominal constrictions in acetic acid-treated mice and reduced paw licking induced by formalin, both in a dose-dependent manner. An acute toxicological evaluation demonstrated no toxic effects after 14 days from LJ and HdLP administration by oral route even when high doses were tested. A qualitative phytochemical analysis showed the absence of lupeol and presence of saponinas, tannins and free steroids in HdLP. However, pharmacological properties are probably not related to them. It is therefore concluded that the latex of Himatanthus drasticus exhibits both the anti-inflammatory and anti-nociceptive activities claimed by its users. The protein fraction of the latex is an important contributor to the pharmacological properties of LJ. Resistance to heat and proteolytic treatment can explain the effectiveness of HdLP even when administered orally. The absence of toxic effects by oral route confirms the potential use of this plant as a phytotherapic agent. |
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Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel)Biochemical aspects of ethnopharmacological and latex Himatanthus drasticus Mart. (Plumel)BioquímicaHimatanthus drasticusLátexAnti-inflamaçãoAntinocicepçãoToxicologiaProteínas laticíferasHimatanthus drasticusLatexAnti-inflammationAnti-nociceptionToxicologyLatex proteinsPlantas medicinais - Uso terapêuticoAgentes anti-inflamatóriosMatéria médica vegetalFitoterapiaHimatanthus drasticus (Apocynaceae) is traditionally used in folk medicine in Northeastern Brazil. The plant is wildly distribuited from Guianas until Southeast Brazil. Latex obtained by cutting its stem bark is mixed with water and extensively sold in public markets. People use Janaguba milk (“Leite de Janaguba”- LJ) to treat or prevent different inflammatory disorders such as gastritis, ulcers as well as cancer, among other uses. Despite, there is not enough scientific data confirming these pharmacological properties. In this work, Janaguba milk was preliminarily investigated in an attempt to validate its anti- inflammatory activity. Its major protein fraction (HdLP) was assessed for the presence of anti- inflammatory, anti-nociceptive and toxicological effects. LJ inhibited the inflammatory response induced by carrageenan in rats (98%, p < 0.05), whereas HdLP did it independent of the route of administration. HdLP significantly reduced the infiltration of neutrophils into the peritoneal cavity (96%, p < 0.05) concomitant with increased nitric oxide synthesis in plasma and decrease of pro-inflammatory cytokines IL- 1 and TNF-α in peritoneal fluid. This fraction also prevented vacuolization and Kupfer cell hyperplasia caused by carrageenan in liver. Further, the anti-inflammatory properties were shown to be associated with the protein fraction of LJ. HdLP exhibited anti-inflammation, even after heat-treatment (100 °C, 30 min) or proteolysis. Moreover, a pro-inflammatory effect was observed after HdLP treatment. It also suppressed abdominal constrictions in acetic acid-treated mice and reduced paw licking induced by formalin, both in a dose-dependent manner. An acute toxicological evaluation demonstrated no toxic effects after 14 days from LJ and HdLP administration by oral route even when high doses were tested. A qualitative phytochemical analysis showed the absence of lupeol and presence of saponinas, tannins and free steroids in HdLP. However, pharmacological properties are probably not related to them. It is therefore concluded that the latex of Himatanthus drasticus exhibits both the anti-inflammatory and anti-nociceptive activities claimed by its users. The protein fraction of the latex is an important contributor to the pharmacological properties of LJ. Resistance to heat and proteolytic treatment can explain the effectiveness of HdLP even when administered orally. The absence of toxic effects by oral route confirms the potential use of this plant as a phytotherapic agent.Himatanthus drasticus (Apocynaceae) é uma espécie tradicionalmente utilizada na medicina popular da região nordeste deste país. Sua distribuição ocorre das Guianas ao sudeste do Brasil. O látex desta planta é obtido após uma injúria no caule e amplamente comercializado em mercados públicos. As pessoas ingerem o látex, conhecido como Leite de Janaguba (LJ), para o tratamento e/ou prevenção de diferentes doenças inflamatórias, gastrite, úlcera, câncer, entre outras. Apesar das diversas informações oriundas da medicina popular, não existem relatos científicos suficientes confirmando tais propriedades farmacológicas. Neste trabalho, LJ foi investigado com o propósito de validar sua tão popularmente relatada atividade anti-inflamatória. A fração proteica deste látex (HdLP) também foi investigada quanto à presença de atividades anti-inflamatória, antinociceptiva, além de seus efeitos toxicológicos. LJ inibiu a resposta inflamatória induzida por carragenina em ratos (98%, p < 0.05), assim como o fez a fração HdLP, independente da rota de administração. HdLP reduziu a infiltração de neutrófilos na cavidade peritoneal (96%, p < 0.05). Paralelamente a isto, ocorreu o aumento da síntese de óxido nítrico no plasma e a redução de citocinas pró-inflamatórias (IL-1 e TNF-α) no fluido peritonal. Esta fração também preveniu a vacuolização e hiperplasia das células de Kupfer, causadas pelo efeito da carragenina no fígado. Atividade anti-inflamatória está provavelmente associada com a fração proteica de LJ. HdLP exibiu um efeito anti-inflamatório mesmo após aquecimento (100 °C, 30 minutos) ou proteólise. Além desta atividade, um efeito pró-inflamatório foi observado após o tratamento com HdLP. Esta também suprimiu contrações abdominais induzidas por ácido acético e reduziu a lambedura de pata induzida por formalina, ambos de maneira dose-dependente. A avaliação da toxicidade aguda demonstrou a ausência de efeitos tóxicos após tratamento com LJ e HdLP (v.o.) mesmo utilizando doses 5000 vezes maiores que as recomendadas. Uma dosagem qualitativa de fitoquímicos em HdLP mostrou a ausência de lupeol e presença de saponinas, taninos e esteróides livres. Entretanto, as propriedades farmacológicas provavelmente não estão relacionadas com estas moléculas. Desta forma, concluímos que o látex de H. drasticus exibe duas das atividades farmacológicas relatadas por seus usuários e a fração proteica deste látex é um importante contribuinte para tais propriedades medicinais. A resistência ao aquecimento e à proteólise pode explicar a efetividade de HdLP quando administrada oralmente. A ausência de efeitos tóxicos por via oral torna esta planta uma potencial candidata a fitoterápico.Ramos, Márcio VianaMatos, Mayara Patrícia Viana de2016-07-26T20:01:21Z2016-07-26T20:01:21Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfMATOS, Mayara Patrícia Viana de. Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel). 2013. 89 f. Dissertação (Mestrado) - Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Bioquímica. Fortaleza, 2013.http://www.repositorio.ufc.br/handle/riufc/18722porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2020-05-20T13:28:49Zoai:repositorio.ufc.br:riufc/18722Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:56:34.810908Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) Biochemical aspects of ethnopharmacological and latex Himatanthus drasticus Mart. (Plumel) |
title |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) |
spellingShingle |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) Matos, Mayara Patrícia Viana de Bioquímica Himatanthus drasticus Látex Anti-inflamação Antinocicepção Toxicologia Proteínas laticíferas Himatanthus drasticus Latex Anti-inflammation Anti-nociception Toxicology Latex proteins Plantas medicinais - Uso terapêutico Agentes anti-inflamatórios Matéria médica vegetal Fitoterapia |
title_short |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) |
title_full |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) |
title_fullStr |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) |
title_full_unstemmed |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) |
title_sort |
Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel) |
author |
Matos, Mayara Patrícia Viana de |
author_facet |
Matos, Mayara Patrícia Viana de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ramos, Márcio Viana |
dc.contributor.author.fl_str_mv |
Matos, Mayara Patrícia Viana de |
dc.subject.por.fl_str_mv |
Bioquímica Himatanthus drasticus Látex Anti-inflamação Antinocicepção Toxicologia Proteínas laticíferas Himatanthus drasticus Latex Anti-inflammation Anti-nociception Toxicology Latex proteins Plantas medicinais - Uso terapêutico Agentes anti-inflamatórios Matéria médica vegetal Fitoterapia |
topic |
Bioquímica Himatanthus drasticus Látex Anti-inflamação Antinocicepção Toxicologia Proteínas laticíferas Himatanthus drasticus Latex Anti-inflammation Anti-nociception Toxicology Latex proteins Plantas medicinais - Uso terapêutico Agentes anti-inflamatórios Matéria médica vegetal Fitoterapia |
description |
Himatanthus drasticus (Apocynaceae) is traditionally used in folk medicine in Northeastern Brazil. The plant is wildly distribuited from Guianas until Southeast Brazil. Latex obtained by cutting its stem bark is mixed with water and extensively sold in public markets. People use Janaguba milk (“Leite de Janaguba”- LJ) to treat or prevent different inflammatory disorders such as gastritis, ulcers as well as cancer, among other uses. Despite, there is not enough scientific data confirming these pharmacological properties. In this work, Janaguba milk was preliminarily investigated in an attempt to validate its anti- inflammatory activity. Its major protein fraction (HdLP) was assessed for the presence of anti- inflammatory, anti-nociceptive and toxicological effects. LJ inhibited the inflammatory response induced by carrageenan in rats (98%, p < 0.05), whereas HdLP did it independent of the route of administration. HdLP significantly reduced the infiltration of neutrophils into the peritoneal cavity (96%, p < 0.05) concomitant with increased nitric oxide synthesis in plasma and decrease of pro-inflammatory cytokines IL- 1 and TNF-α in peritoneal fluid. This fraction also prevented vacuolization and Kupfer cell hyperplasia caused by carrageenan in liver. Further, the anti-inflammatory properties were shown to be associated with the protein fraction of LJ. HdLP exhibited anti-inflammation, even after heat-treatment (100 °C, 30 min) or proteolysis. Moreover, a pro-inflammatory effect was observed after HdLP treatment. It also suppressed abdominal constrictions in acetic acid-treated mice and reduced paw licking induced by formalin, both in a dose-dependent manner. An acute toxicological evaluation demonstrated no toxic effects after 14 days from LJ and HdLP administration by oral route even when high doses were tested. A qualitative phytochemical analysis showed the absence of lupeol and presence of saponinas, tannins and free steroids in HdLP. However, pharmacological properties are probably not related to them. It is therefore concluded that the latex of Himatanthus drasticus exhibits both the anti-inflammatory and anti-nociceptive activities claimed by its users. The protein fraction of the latex is an important contributor to the pharmacological properties of LJ. Resistance to heat and proteolytic treatment can explain the effectiveness of HdLP even when administered orally. The absence of toxic effects by oral route confirms the potential use of this plant as a phytotherapic agent. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2016-07-26T20:01:21Z 2016-07-26T20:01:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MATOS, Mayara Patrícia Viana de. Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel). 2013. 89 f. Dissertação (Mestrado) - Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Bioquímica. Fortaleza, 2013. http://www.repositorio.ufc.br/handle/riufc/18722 |
identifier_str_mv |
MATOS, Mayara Patrícia Viana de. Aspectos bioquímicos e etnofarmacológicos do látex de Himatanthus drasticus Mart. (Plumel). 2013. 89 f. Dissertação (Mestrado) - Universidade Federal do Ceará, Centro de Ciências, Departamento de Bioquímica e Biologia Molecular, Programa de Pós-Graduação em Bioquímica. Fortaleza, 2013. |
url |
http://www.repositorio.ufc.br/handle/riufc/18722 |
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openAccess |
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Universidade Federal do Ceará (UFC) |
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UFC |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
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