Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma

Detalhes bibliográficos
Autor(a) principal: Teixeira, Dárcio Ítalo Alves
Data de Publicação: 2006
Outros Autores: Melo, Luciana Magalhães, Gadelha, Carlos Alberto de Almeida, Cunha, Rodrigo Maranguape Silva da, Bloch Júnior, Carlos, Rádis-Baptista, Gandhi, Cavada, Benildo Sousa, Freitas, Vicente José de Figueirêdo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/59840
Resumo: Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-exchange chromatography to isolate this seminal protein. Semen was obtained from four adult Saanen bucks. Seminal plasma was pooled, dialyzed against distilled water and freeze-dried. Lyophilized proteins were loaded onto an ion-exchange chromatography column. Dialyzed-lyophilized proteins from the main peak of DEAE-Sephacel were applied to a C2/C18 column coupled to an RP-HPLC system, and the eluted proteins were lyophilized for electrophoresis. The N-terminal was sequenced and amino acid sequence similarity was determined using CLUSTAL W. Additionally, proteins from DEAE-Sephacel chromatography step were dialyzed and submitted to a heparin-Sepharose highperformance liquid chromatography. Goat seminal plasma after ion-exchange chromatography yielded 6.47 ± 0.63 mg (mean ± SEM) of the major retained fraction. The protein was designated BSFP (buck seminal fluid protein). BSFP exhibited N-terminal sequence homology to boar, stallion and bull spermadhesins. BSFP showed no heparin-binding capabilities. These results together with our previous data indicate that goat seminal plasma contains a protein that is structurally related to proteins of the spermadhesin family. Finally, this protein can be efficiently isolated by ion-exchange and reverse-phase chromatography
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spelling Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasmaIon-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasmaPlasma seminalOvinosLecitinaMammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-exchange chromatography to isolate this seminal protein. Semen was obtained from four adult Saanen bucks. Seminal plasma was pooled, dialyzed against distilled water and freeze-dried. Lyophilized proteins were loaded onto an ion-exchange chromatography column. Dialyzed-lyophilized proteins from the main peak of DEAE-Sephacel were applied to a C2/C18 column coupled to an RP-HPLC system, and the eluted proteins were lyophilized for electrophoresis. The N-terminal was sequenced and amino acid sequence similarity was determined using CLUSTAL W. Additionally, proteins from DEAE-Sephacel chromatography step were dialyzed and submitted to a heparin-Sepharose highperformance liquid chromatography. Goat seminal plasma after ion-exchange chromatography yielded 6.47 ± 0.63 mg (mean ± SEM) of the major retained fraction. The protein was designated BSFP (buck seminal fluid protein). BSFP exhibited N-terminal sequence homology to boar, stallion and bull spermadhesins. BSFP showed no heparin-binding capabilities. These results together with our previous data indicate that goat seminal plasma contains a protein that is structurally related to proteins of the spermadhesin family. Finally, this protein can be efficiently isolated by ion-exchange and reverse-phase chromatographyGenetics and Molecular Research2021-08-04T12:08:31Z2021-08-04T12:08:31Z2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfTEIXEIRA, Dárcio Ítalo Alves; MELO, Luciana Magalhães; GADELHA, Carlos Alberto de Almeida; CUNHA, Rodrigo Maranguape Silva da; BLOCH JÚNIOR, Cunha; RÁDIS-BAPTISTA, Gandhi; CAVADA, Benildo Sousa; FREITAS, Vicente José de Figueirêdo. Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma. Genetics and Molecular Research, v. 5, n.1, p. 79-87. 2006. Disponível em: https://www.academia.edu/4589075/Ion_exchange_chromatography_used_to_isolate_a_ spermadhesin_related_protein_from_domestic_goat_Capra_hircus_seminal_plasma. Acesso em: 4 ago. 20211676-5680http://www.repositorio.ufc.br/handle/riufc/59840Teixeira, Dárcio Ítalo AlvesMelo, Luciana MagalhãesGadelha, Carlos Alberto de AlmeidaCunha, Rodrigo Maranguape Silva daBloch Júnior, CarlosRádis-Baptista, GandhiCavada, Benildo SousaFreitas, Vicente José de Figueirêdoengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-12-16T13:32:34Zoai:repositorio.ufc.br:riufc/59840Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:18:56.103687Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
title Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
spellingShingle Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
Teixeira, Dárcio Ítalo Alves
Plasma seminal
Ovinos
Lecitina
title_short Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
title_full Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
title_fullStr Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
title_full_unstemmed Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
title_sort Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
author Teixeira, Dárcio Ítalo Alves
author_facet Teixeira, Dárcio Ítalo Alves
Melo, Luciana Magalhães
Gadelha, Carlos Alberto de Almeida
Cunha, Rodrigo Maranguape Silva da
Bloch Júnior, Carlos
Rádis-Baptista, Gandhi
Cavada, Benildo Sousa
Freitas, Vicente José de Figueirêdo
author_role author
author2 Melo, Luciana Magalhães
Gadelha, Carlos Alberto de Almeida
Cunha, Rodrigo Maranguape Silva da
Bloch Júnior, Carlos
Rádis-Baptista, Gandhi
Cavada, Benildo Sousa
Freitas, Vicente José de Figueirêdo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira, Dárcio Ítalo Alves
Melo, Luciana Magalhães
Gadelha, Carlos Alberto de Almeida
Cunha, Rodrigo Maranguape Silva da
Bloch Júnior, Carlos
Rádis-Baptista, Gandhi
Cavada, Benildo Sousa
Freitas, Vicente José de Figueirêdo
dc.subject.por.fl_str_mv Plasma seminal
Ovinos
Lecitina
topic Plasma seminal
Ovinos
Lecitina
description Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-exchange chromatography to isolate this seminal protein. Semen was obtained from four adult Saanen bucks. Seminal plasma was pooled, dialyzed against distilled water and freeze-dried. Lyophilized proteins were loaded onto an ion-exchange chromatography column. Dialyzed-lyophilized proteins from the main peak of DEAE-Sephacel were applied to a C2/C18 column coupled to an RP-HPLC system, and the eluted proteins were lyophilized for electrophoresis. The N-terminal was sequenced and amino acid sequence similarity was determined using CLUSTAL W. Additionally, proteins from DEAE-Sephacel chromatography step were dialyzed and submitted to a heparin-Sepharose highperformance liquid chromatography. Goat seminal plasma after ion-exchange chromatography yielded 6.47 ± 0.63 mg (mean ± SEM) of the major retained fraction. The protein was designated BSFP (buck seminal fluid protein). BSFP exhibited N-terminal sequence homology to boar, stallion and bull spermadhesins. BSFP showed no heparin-binding capabilities. These results together with our previous data indicate that goat seminal plasma contains a protein that is structurally related to proteins of the spermadhesin family. Finally, this protein can be efficiently isolated by ion-exchange and reverse-phase chromatography
publishDate 2006
dc.date.none.fl_str_mv 2006
2021-08-04T12:08:31Z
2021-08-04T12:08:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv TEIXEIRA, Dárcio Ítalo Alves; MELO, Luciana Magalhães; GADELHA, Carlos Alberto de Almeida; CUNHA, Rodrigo Maranguape Silva da; BLOCH JÚNIOR, Cunha; RÁDIS-BAPTISTA, Gandhi; CAVADA, Benildo Sousa; FREITAS, Vicente José de Figueirêdo. Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma. Genetics and Molecular Research, v. 5, n.1, p. 79-87. 2006. Disponível em: https://www.academia.edu/4589075/Ion_exchange_chromatography_used_to_isolate_a_ spermadhesin_related_protein_from_domestic_goat_Capra_hircus_seminal_plasma. Acesso em: 4 ago. 2021
1676-5680
http://www.repositorio.ufc.br/handle/riufc/59840
identifier_str_mv TEIXEIRA, Dárcio Ítalo Alves; MELO, Luciana Magalhães; GADELHA, Carlos Alberto de Almeida; CUNHA, Rodrigo Maranguape Silva da; BLOCH JÚNIOR, Cunha; RÁDIS-BAPTISTA, Gandhi; CAVADA, Benildo Sousa; FREITAS, Vicente José de Figueirêdo. Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma. Genetics and Molecular Research, v. 5, n.1, p. 79-87. 2006. Disponível em: https://www.academia.edu/4589075/Ion_exchange_chromatography_used_to_isolate_a_ spermadhesin_related_protein_from_domestic_goat_Capra_hircus_seminal_plasma. Acesso em: 4 ago. 2021
1676-5680
url http://www.repositorio.ufc.br/handle/riufc/59840
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Genetics and Molecular Research
publisher.none.fl_str_mv Genetics and Molecular Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
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