Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/59840 |
Resumo: | Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-exchange chromatography to isolate this seminal protein. Semen was obtained from four adult Saanen bucks. Seminal plasma was pooled, dialyzed against distilled water and freeze-dried. Lyophilized proteins were loaded onto an ion-exchange chromatography column. Dialyzed-lyophilized proteins from the main peak of DEAE-Sephacel were applied to a C2/C18 column coupled to an RP-HPLC system, and the eluted proteins were lyophilized for electrophoresis. The N-terminal was sequenced and amino acid sequence similarity was determined using CLUSTAL W. Additionally, proteins from DEAE-Sephacel chromatography step were dialyzed and submitted to a heparin-Sepharose highperformance liquid chromatography. Goat seminal plasma after ion-exchange chromatography yielded 6.47 ± 0.63 mg (mean ± SEM) of the major retained fraction. The protein was designated BSFP (buck seminal fluid protein). BSFP exhibited N-terminal sequence homology to boar, stallion and bull spermadhesins. BSFP showed no heparin-binding capabilities. These results together with our previous data indicate that goat seminal plasma contains a protein that is structurally related to proteins of the spermadhesin family. Finally, this protein can be efficiently isolated by ion-exchange and reverse-phase chromatography |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasmaIon-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasmaPlasma seminalOvinosLecitinaMammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-exchange chromatography to isolate this seminal protein. Semen was obtained from four adult Saanen bucks. Seminal plasma was pooled, dialyzed against distilled water and freeze-dried. Lyophilized proteins were loaded onto an ion-exchange chromatography column. Dialyzed-lyophilized proteins from the main peak of DEAE-Sephacel were applied to a C2/C18 column coupled to an RP-HPLC system, and the eluted proteins were lyophilized for electrophoresis. The N-terminal was sequenced and amino acid sequence similarity was determined using CLUSTAL W. Additionally, proteins from DEAE-Sephacel chromatography step were dialyzed and submitted to a heparin-Sepharose highperformance liquid chromatography. Goat seminal plasma after ion-exchange chromatography yielded 6.47 ± 0.63 mg (mean ± SEM) of the major retained fraction. The protein was designated BSFP (buck seminal fluid protein). BSFP exhibited N-terminal sequence homology to boar, stallion and bull spermadhesins. BSFP showed no heparin-binding capabilities. These results together with our previous data indicate that goat seminal plasma contains a protein that is structurally related to proteins of the spermadhesin family. Finally, this protein can be efficiently isolated by ion-exchange and reverse-phase chromatographyGenetics and Molecular Research2021-08-04T12:08:31Z2021-08-04T12:08:31Z2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfTEIXEIRA, Dárcio Ítalo Alves; MELO, Luciana Magalhães; GADELHA, Carlos Alberto de Almeida; CUNHA, Rodrigo Maranguape Silva da; BLOCH JÚNIOR, Cunha; RÁDIS-BAPTISTA, Gandhi; CAVADA, Benildo Sousa; FREITAS, Vicente José de Figueirêdo. Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma. Genetics and Molecular Research, v. 5, n.1, p. 79-87. 2006. Disponível em: https://www.academia.edu/4589075/Ion_exchange_chromatography_used_to_isolate_a_ spermadhesin_related_protein_from_domestic_goat_Capra_hircus_seminal_plasma. Acesso em: 4 ago. 20211676-5680http://www.repositorio.ufc.br/handle/riufc/59840Teixeira, Dárcio Ítalo AlvesMelo, Luciana MagalhãesGadelha, Carlos Alberto de AlmeidaCunha, Rodrigo Maranguape Silva daBloch Júnior, CarlosRádis-Baptista, GandhiCavada, Benildo SousaFreitas, Vicente José de Figueirêdoengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-12-16T13:32:34Zoai:repositorio.ufc.br:riufc/59840Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:18:56.103687Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma |
title |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma |
spellingShingle |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma Teixeira, Dárcio Ítalo Alves Plasma seminal Ovinos Lecitina |
title_short |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma |
title_full |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma |
title_fullStr |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma |
title_full_unstemmed |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma |
title_sort |
Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma |
author |
Teixeira, Dárcio Ítalo Alves |
author_facet |
Teixeira, Dárcio Ítalo Alves Melo, Luciana Magalhães Gadelha, Carlos Alberto de Almeida Cunha, Rodrigo Maranguape Silva da Bloch Júnior, Carlos Rádis-Baptista, Gandhi Cavada, Benildo Sousa Freitas, Vicente José de Figueirêdo |
author_role |
author |
author2 |
Melo, Luciana Magalhães Gadelha, Carlos Alberto de Almeida Cunha, Rodrigo Maranguape Silva da Bloch Júnior, Carlos Rádis-Baptista, Gandhi Cavada, Benildo Sousa Freitas, Vicente José de Figueirêdo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Teixeira, Dárcio Ítalo Alves Melo, Luciana Magalhães Gadelha, Carlos Alberto de Almeida Cunha, Rodrigo Maranguape Silva da Bloch Júnior, Carlos Rádis-Baptista, Gandhi Cavada, Benildo Sousa Freitas, Vicente José de Figueirêdo |
dc.subject.por.fl_str_mv |
Plasma seminal Ovinos Lecitina |
topic |
Plasma seminal Ovinos Lecitina |
description |
Mammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-exchange chromatography to isolate this seminal protein. Semen was obtained from four adult Saanen bucks. Seminal plasma was pooled, dialyzed against distilled water and freeze-dried. Lyophilized proteins were loaded onto an ion-exchange chromatography column. Dialyzed-lyophilized proteins from the main peak of DEAE-Sephacel were applied to a C2/C18 column coupled to an RP-HPLC system, and the eluted proteins were lyophilized for electrophoresis. The N-terminal was sequenced and amino acid sequence similarity was determined using CLUSTAL W. Additionally, proteins from DEAE-Sephacel chromatography step were dialyzed and submitted to a heparin-Sepharose highperformance liquid chromatography. Goat seminal plasma after ion-exchange chromatography yielded 6.47 ± 0.63 mg (mean ± SEM) of the major retained fraction. The protein was designated BSFP (buck seminal fluid protein). BSFP exhibited N-terminal sequence homology to boar, stallion and bull spermadhesins. BSFP showed no heparin-binding capabilities. These results together with our previous data indicate that goat seminal plasma contains a protein that is structurally related to proteins of the spermadhesin family. Finally, this protein can be efficiently isolated by ion-exchange and reverse-phase chromatography |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2021-08-04T12:08:31Z 2021-08-04T12:08:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
TEIXEIRA, Dárcio Ítalo Alves; MELO, Luciana Magalhães; GADELHA, Carlos Alberto de Almeida; CUNHA, Rodrigo Maranguape Silva da; BLOCH JÚNIOR, Cunha; RÁDIS-BAPTISTA, Gandhi; CAVADA, Benildo Sousa; FREITAS, Vicente José de Figueirêdo. Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma. Genetics and Molecular Research, v. 5, n.1, p. 79-87. 2006. Disponível em: https://www.academia.edu/4589075/Ion_exchange_chromatography_used_to_isolate_a_ spermadhesin_related_protein_from_domestic_goat_Capra_hircus_seminal_plasma. Acesso em: 4 ago. 2021 1676-5680 http://www.repositorio.ufc.br/handle/riufc/59840 |
identifier_str_mv |
TEIXEIRA, Dárcio Ítalo Alves; MELO, Luciana Magalhães; GADELHA, Carlos Alberto de Almeida; CUNHA, Rodrigo Maranguape Silva da; BLOCH JÚNIOR, Cunha; RÁDIS-BAPTISTA, Gandhi; CAVADA, Benildo Sousa; FREITAS, Vicente José de Figueirêdo. Ion-exchange chromatography used to isolate a spermadhesin-related protein from domestic goat (Capra hircus) seminal plasma. Genetics and Molecular Research, v. 5, n.1, p. 79-87. 2006. Disponível em: https://www.academia.edu/4589075/Ion_exchange_chromatography_used_to_isolate_a_ spermadhesin_related_protein_from_domestic_goat_Capra_hircus_seminal_plasma. Acesso em: 4 ago. 2021 1676-5680 |
url |
http://www.repositorio.ufc.br/handle/riufc/59840 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Genetics and Molecular Research |
publisher.none.fl_str_mv |
Genetics and Molecular Research |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1813028751241379840 |