Caracterização ecoepidemiológica de micobactérias em triatomíneos coletados no município de Sobral-Ceará

• Autor(a) principal: Silva, Caroliny Soares
• Data de Publicação: 2021
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/59010
• topic: Triatominae; Mycobacterium; Tuberculose

INTRODUCTION: The Mycobacteriaceae family is formed by the genus Mycobacterium and four new genera. Noteworthy are the species of the Mycobacterium tuberculosis complex (CMTB) that cause tuberculosis (TB), the species collectively called Non-Tuberculous Mycobacteria (NTM) that cause mycobacteriosis, and the causative agent of leprosy, M. leprae. Studies conducted in Sobral, Ceará, found several species of triatomines within the urban perimeter. The intestinal microbiota of these insects is dynamic and diverse, including species of the genus Mycobacterium. OBJECTIVE: To identify the presence of mycobacteria in triatomine hemiptera found in homes of the urban and rural areas of the city of Sobral, Ceará. METHODS: The triatomines used in this study were provided by the Sobral Zoonoses Control Center. The insects were captured from January 2019 to April 2020 and sent to Triatomine Information Posts installed at the municipal health posts, for taxonomic characterization and storage. Genomic DNA extraction of the collected triatomines was performed, followed by identification of mycobacteria using the PRA-hsp65 technique. DNA samples from PRA-hsp65 positive triatomines were sequenced and then phylogenetically analyzed. The addresses of the places where triatomines were collected and the residences of TB cases diagnosed in 2018-2019 were spatially analyzed. RESULTS: It was collected 167 samples of triatomines, from the total 41.9% (70/167) were PRA-hsp65 positive for the genus Mycobacterium. Sequences were obtained from 70% (49/70) of the samples, being identified 12 bacterial genera, including 9 samples as CMTB. Spatial analysis found two significant clusters of triatomines and one cluster of TB cases. Spatial interpolation indicated a high-risk trend of spatial agglomeration in the county seat for triatomines and TB cases. CONCLUSION: This study is the first to describe the finding of CMTB species in triatomines in the urban peridomicile. The set of information from this study can be used to understand the possible transmission of mycobacteria by triatomines, and thus help to establish adequate measures for the prevention of mycobacteriosis.