Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis

Detalhes bibliográficos
Autor(a) principal: Araújo, Aurigena Antunes de
Data de Publicação: 2017
Outros Autores: Pereira, Aline de Sousa Barbosa Freitas, Medeiros, Caroline Addison Carvalho Xavier de, Brito, Gerly Anne de Castro, Leitão, Renata Ferreira de Carvalho, Araújo, Lorena de Souza, Guedes, Paulo Marcos Matta, Hiyari, Sarah, Pirih, Flávia Q., Araújo Júnior, Raimundo Fernandes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/28053
Resumo: Aim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model of ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups of twenty-one rats each, and given the following treatments for 10 days: (1) no ligature + water, (2) ligature + water, (3) ligature + 50 mg/kg Met, (4) ligature + 100 mg/kg Met, and (5) ligature + 200 mg/kg Met. Water or Met was administered orally. Maxillae were fixed and scanned using Micro-computed Tomography (μCT) to quantitate linear and bone volume/tissue volume (BV/TV) volumetric bone loss. Histopathological characteristics were assessed through immunohistochemical staining for MMP-9, COX-2, the RANKL/RANK/OPG pathway, SOD-1, and GPx-1. Additionally, confocal microscopy was used to analyze osteocalcin fluorescence. UV-VIS analysis was used to examine the levels of malondialdehyde, glutathione, IL-1β and TNF-α from gingival tissues. Quantitative RT-PCR reaction was used to gene expression of AMPK, NF-κB (p65), and Hmgb1 from gingival tissues. Significance among groups were analysed using a one-way ANOVA. A p-value of p<0.05 indicated a significant difference. Results Treatment with 50 mg/kg Met significantly reduced concentrations of malondialdehyde, IL-1β, and TNF-α (p < 0.05). Additionally, weak staining was observed for COX-2, MMP-9, RANK, RANKL, SOD-1, and GPx-1 after 50 mg/kg Met. OPG and Osteocalcin showed strong staining in the same group. Radiographically, linear measurements showed a statistically significant reduction in bone loss after 50 mg/kg Met compared to the ligature and Met 200 mg/kg groups. The same pattern was observed volumetrically in BV/TV and decreased osteoclast number (p<0.05). RT-PCR showed increased AMPK expression and decreased expression of NF-κB (p65) and HMGB1 after 50 mg/kg Met. Conclusions Metformin, at a concentration of 50 mg/kg, decreases the inflammatory response, oxidative stress and bone loss in ligature-induced periodontitis in rats.
id UFC-7_3dbdf6dea8047d57eb2d94f8c8a2445f
oai_identifier_str oai:repositorio.ufc.br:riufc/28053
network_acronym_str UFC-7
network_name_str Repositório Institucional da Universidade Federal do Ceará (UFC)
repository_id_str
spelling Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitisEstresse OxidativoMetforminaMetforminAim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model of ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups of twenty-one rats each, and given the following treatments for 10 days: (1) no ligature + water, (2) ligature + water, (3) ligature + 50 mg/kg Met, (4) ligature + 100 mg/kg Met, and (5) ligature + 200 mg/kg Met. Water or Met was administered orally. Maxillae were fixed and scanned using Micro-computed Tomography (μCT) to quantitate linear and bone volume/tissue volume (BV/TV) volumetric bone loss. Histopathological characteristics were assessed through immunohistochemical staining for MMP-9, COX-2, the RANKL/RANK/OPG pathway, SOD-1, and GPx-1. Additionally, confocal microscopy was used to analyze osteocalcin fluorescence. UV-VIS analysis was used to examine the levels of malondialdehyde, glutathione, IL-1β and TNF-α from gingival tissues. Quantitative RT-PCR reaction was used to gene expression of AMPK, NF-κB (p65), and Hmgb1 from gingival tissues. Significance among groups were analysed using a one-way ANOVA. A p-value of p<0.05 indicated a significant difference. Results Treatment with 50 mg/kg Met significantly reduced concentrations of malondialdehyde, IL-1β, and TNF-α (p < 0.05). Additionally, weak staining was observed for COX-2, MMP-9, RANK, RANKL, SOD-1, and GPx-1 after 50 mg/kg Met. OPG and Osteocalcin showed strong staining in the same group. Radiographically, linear measurements showed a statistically significant reduction in bone loss after 50 mg/kg Met compared to the ligature and Met 200 mg/kg groups. The same pattern was observed volumetrically in BV/TV and decreased osteoclast number (p<0.05). RT-PCR showed increased AMPK expression and decreased expression of NF-κB (p65) and HMGB1 after 50 mg/kg Met. Conclusions Metformin, at a concentration of 50 mg/kg, decreases the inflammatory response, oxidative stress and bone loss in ligature-induced periodontitis in rats.PLoS One2017-11-28T14:05:07Z2017-11-28T14:05:07Z2017-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfARAÚJO, A. A. de et al. Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One, v. 12, p. 1-21, aug. 2017.1932-6203 (Online)http://www.repositorio.ufc.br/handle/riufc/28053Araújo, Aurigena Antunes dePereira, Aline de Sousa Barbosa FreitasMedeiros, Caroline Addison Carvalho Xavier deBrito, Gerly Anne de CastroLeitão, Renata Ferreira de CarvalhoAraújo, Lorena de SouzaGuedes, Paulo Marcos MattaHiyari, SarahPirih, Flávia Q.Araújo Júnior, Raimundo Fernandes deengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-10-16T17:53:57Zoai:repositorio.ufc.br:riufc/28053Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:25:43.317462Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
title Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
spellingShingle Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
Araújo, Aurigena Antunes de
Estresse Oxidativo
Metformina
Metformin
title_short Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
title_full Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
title_fullStr Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
title_full_unstemmed Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
title_sort Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
author Araújo, Aurigena Antunes de
author_facet Araújo, Aurigena Antunes de
Pereira, Aline de Sousa Barbosa Freitas
Medeiros, Caroline Addison Carvalho Xavier de
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira de Carvalho
Araújo, Lorena de Souza
Guedes, Paulo Marcos Matta
Hiyari, Sarah
Pirih, Flávia Q.
Araújo Júnior, Raimundo Fernandes de
author_role author
author2 Pereira, Aline de Sousa Barbosa Freitas
Medeiros, Caroline Addison Carvalho Xavier de
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira de Carvalho
Araújo, Lorena de Souza
Guedes, Paulo Marcos Matta
Hiyari, Sarah
Pirih, Flávia Q.
Araújo Júnior, Raimundo Fernandes de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araújo, Aurigena Antunes de
Pereira, Aline de Sousa Barbosa Freitas
Medeiros, Caroline Addison Carvalho Xavier de
Brito, Gerly Anne de Castro
Leitão, Renata Ferreira de Carvalho
Araújo, Lorena de Souza
Guedes, Paulo Marcos Matta
Hiyari, Sarah
Pirih, Flávia Q.
Araújo Júnior, Raimundo Fernandes de
dc.subject.por.fl_str_mv Estresse Oxidativo
Metformina
Metformin
topic Estresse Oxidativo
Metformina
Metformin
description Aim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model of ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups of twenty-one rats each, and given the following treatments for 10 days: (1) no ligature + water, (2) ligature + water, (3) ligature + 50 mg/kg Met, (4) ligature + 100 mg/kg Met, and (5) ligature + 200 mg/kg Met. Water or Met was administered orally. Maxillae were fixed and scanned using Micro-computed Tomography (μCT) to quantitate linear and bone volume/tissue volume (BV/TV) volumetric bone loss. Histopathological characteristics were assessed through immunohistochemical staining for MMP-9, COX-2, the RANKL/RANK/OPG pathway, SOD-1, and GPx-1. Additionally, confocal microscopy was used to analyze osteocalcin fluorescence. UV-VIS analysis was used to examine the levels of malondialdehyde, glutathione, IL-1β and TNF-α from gingival tissues. Quantitative RT-PCR reaction was used to gene expression of AMPK, NF-κB (p65), and Hmgb1 from gingival tissues. Significance among groups were analysed using a one-way ANOVA. A p-value of p<0.05 indicated a significant difference. Results Treatment with 50 mg/kg Met significantly reduced concentrations of malondialdehyde, IL-1β, and TNF-α (p < 0.05). Additionally, weak staining was observed for COX-2, MMP-9, RANK, RANKL, SOD-1, and GPx-1 after 50 mg/kg Met. OPG and Osteocalcin showed strong staining in the same group. Radiographically, linear measurements showed a statistically significant reduction in bone loss after 50 mg/kg Met compared to the ligature and Met 200 mg/kg groups. The same pattern was observed volumetrically in BV/TV and decreased osteoclast number (p<0.05). RT-PCR showed increased AMPK expression and decreased expression of NF-κB (p65) and HMGB1 after 50 mg/kg Met. Conclusions Metformin, at a concentration of 50 mg/kg, decreases the inflammatory response, oxidative stress and bone loss in ligature-induced periodontitis in rats.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-28T14:05:07Z
2017-11-28T14:05:07Z
2017-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ARAÚJO, A. A. de et al. Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One, v. 12, p. 1-21, aug. 2017.
1932-6203 (Online)
http://www.repositorio.ufc.br/handle/riufc/28053
identifier_str_mv ARAÚJO, A. A. de et al. Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One, v. 12, p. 1-21, aug. 2017.
1932-6203 (Online)
url http://www.repositorio.ufc.br/handle/riufc/28053
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLoS One
publisher.none.fl_str_mv PLoS One
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1813028799382552576