Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/28053 |
Resumo: | Aim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model of ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups of twenty-one rats each, and given the following treatments for 10 days: (1) no ligature + water, (2) ligature + water, (3) ligature + 50 mg/kg Met, (4) ligature + 100 mg/kg Met, and (5) ligature + 200 mg/kg Met. Water or Met was administered orally. Maxillae were fixed and scanned using Micro-computed Tomography (μCT) to quantitate linear and bone volume/tissue volume (BV/TV) volumetric bone loss. Histopathological characteristics were assessed through immunohistochemical staining for MMP-9, COX-2, the RANKL/RANK/OPG pathway, SOD-1, and GPx-1. Additionally, confocal microscopy was used to analyze osteocalcin fluorescence. UV-VIS analysis was used to examine the levels of malondialdehyde, glutathione, IL-1β and TNF-α from gingival tissues. Quantitative RT-PCR reaction was used to gene expression of AMPK, NF-κB (p65), and Hmgb1 from gingival tissues. Significance among groups were analysed using a one-way ANOVA. A p-value of p<0.05 indicated a significant difference. Results Treatment with 50 mg/kg Met significantly reduced concentrations of malondialdehyde, IL-1β, and TNF-α (p < 0.05). Additionally, weak staining was observed for COX-2, MMP-9, RANK, RANKL, SOD-1, and GPx-1 after 50 mg/kg Met. OPG and Osteocalcin showed strong staining in the same group. Radiographically, linear measurements showed a statistically significant reduction in bone loss after 50 mg/kg Met compared to the ligature and Met 200 mg/kg groups. The same pattern was observed volumetrically in BV/TV and decreased osteoclast number (p<0.05). RT-PCR showed increased AMPK expression and decreased expression of NF-κB (p65) and HMGB1 after 50 mg/kg Met. Conclusions Metformin, at a concentration of 50 mg/kg, decreases the inflammatory response, oxidative stress and bone loss in ligature-induced periodontitis in rats. |
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Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitisEstresse OxidativoMetforminaMetforminAim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model of ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups of twenty-one rats each, and given the following treatments for 10 days: (1) no ligature + water, (2) ligature + water, (3) ligature + 50 mg/kg Met, (4) ligature + 100 mg/kg Met, and (5) ligature + 200 mg/kg Met. Water or Met was administered orally. Maxillae were fixed and scanned using Micro-computed Tomography (μCT) to quantitate linear and bone volume/tissue volume (BV/TV) volumetric bone loss. Histopathological characteristics were assessed through immunohistochemical staining for MMP-9, COX-2, the RANKL/RANK/OPG pathway, SOD-1, and GPx-1. Additionally, confocal microscopy was used to analyze osteocalcin fluorescence. UV-VIS analysis was used to examine the levels of malondialdehyde, glutathione, IL-1β and TNF-α from gingival tissues. Quantitative RT-PCR reaction was used to gene expression of AMPK, NF-κB (p65), and Hmgb1 from gingival tissues. Significance among groups were analysed using a one-way ANOVA. A p-value of p<0.05 indicated a significant difference. Results Treatment with 50 mg/kg Met significantly reduced concentrations of malondialdehyde, IL-1β, and TNF-α (p < 0.05). Additionally, weak staining was observed for COX-2, MMP-9, RANK, RANKL, SOD-1, and GPx-1 after 50 mg/kg Met. OPG and Osteocalcin showed strong staining in the same group. Radiographically, linear measurements showed a statistically significant reduction in bone loss after 50 mg/kg Met compared to the ligature and Met 200 mg/kg groups. The same pattern was observed volumetrically in BV/TV and decreased osteoclast number (p<0.05). RT-PCR showed increased AMPK expression and decreased expression of NF-κB (p65) and HMGB1 after 50 mg/kg Met. Conclusions Metformin, at a concentration of 50 mg/kg, decreases the inflammatory response, oxidative stress and bone loss in ligature-induced periodontitis in rats.PLoS One2017-11-28T14:05:07Z2017-11-28T14:05:07Z2017-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfARAÚJO, A. A. de et al. Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One, v. 12, p. 1-21, aug. 2017.1932-6203 (Online)http://www.repositorio.ufc.br/handle/riufc/28053Araújo, Aurigena Antunes dePereira, Aline de Sousa Barbosa FreitasMedeiros, Caroline Addison Carvalho Xavier deBrito, Gerly Anne de CastroLeitão, Renata Ferreira de CarvalhoAraújo, Lorena de SouzaGuedes, Paulo Marcos MattaHiyari, SarahPirih, Flávia Q.Araújo Júnior, Raimundo Fernandes deengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-10-16T17:53:57Zoai:repositorio.ufc.br:riufc/28053Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:25:43.317462Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis |
title |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis |
spellingShingle |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis Araújo, Aurigena Antunes de Estresse Oxidativo Metformina Metformin |
title_short |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis |
title_full |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis |
title_fullStr |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis |
title_full_unstemmed |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis |
title_sort |
Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis |
author |
Araújo, Aurigena Antunes de |
author_facet |
Araújo, Aurigena Antunes de Pereira, Aline de Sousa Barbosa Freitas Medeiros, Caroline Addison Carvalho Xavier de Brito, Gerly Anne de Castro Leitão, Renata Ferreira de Carvalho Araújo, Lorena de Souza Guedes, Paulo Marcos Matta Hiyari, Sarah Pirih, Flávia Q. Araújo Júnior, Raimundo Fernandes de |
author_role |
author |
author2 |
Pereira, Aline de Sousa Barbosa Freitas Medeiros, Caroline Addison Carvalho Xavier de Brito, Gerly Anne de Castro Leitão, Renata Ferreira de Carvalho Araújo, Lorena de Souza Guedes, Paulo Marcos Matta Hiyari, Sarah Pirih, Flávia Q. Araújo Júnior, Raimundo Fernandes de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Aurigena Antunes de Pereira, Aline de Sousa Barbosa Freitas Medeiros, Caroline Addison Carvalho Xavier de Brito, Gerly Anne de Castro Leitão, Renata Ferreira de Carvalho Araújo, Lorena de Souza Guedes, Paulo Marcos Matta Hiyari, Sarah Pirih, Flávia Q. Araújo Júnior, Raimundo Fernandes de |
dc.subject.por.fl_str_mv |
Estresse Oxidativo Metformina Metformin |
topic |
Estresse Oxidativo Metformina Metformin |
description |
Aim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model of ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups of twenty-one rats each, and given the following treatments for 10 days: (1) no ligature + water, (2) ligature + water, (3) ligature + 50 mg/kg Met, (4) ligature + 100 mg/kg Met, and (5) ligature + 200 mg/kg Met. Water or Met was administered orally. Maxillae were fixed and scanned using Micro-computed Tomography (μCT) to quantitate linear and bone volume/tissue volume (BV/TV) volumetric bone loss. Histopathological characteristics were assessed through immunohistochemical staining for MMP-9, COX-2, the RANKL/RANK/OPG pathway, SOD-1, and GPx-1. Additionally, confocal microscopy was used to analyze osteocalcin fluorescence. UV-VIS analysis was used to examine the levels of malondialdehyde, glutathione, IL-1β and TNF-α from gingival tissues. Quantitative RT-PCR reaction was used to gene expression of AMPK, NF-κB (p65), and Hmgb1 from gingival tissues. Significance among groups were analysed using a one-way ANOVA. A p-value of p<0.05 indicated a significant difference. Results Treatment with 50 mg/kg Met significantly reduced concentrations of malondialdehyde, IL-1β, and TNF-α (p < 0.05). Additionally, weak staining was observed for COX-2, MMP-9, RANK, RANKL, SOD-1, and GPx-1 after 50 mg/kg Met. OPG and Osteocalcin showed strong staining in the same group. Radiographically, linear measurements showed a statistically significant reduction in bone loss after 50 mg/kg Met compared to the ligature and Met 200 mg/kg groups. The same pattern was observed volumetrically in BV/TV and decreased osteoclast number (p<0.05). RT-PCR showed increased AMPK expression and decreased expression of NF-κB (p65) and HMGB1 after 50 mg/kg Met. Conclusions Metformin, at a concentration of 50 mg/kg, decreases the inflammatory response, oxidative stress and bone loss in ligature-induced periodontitis in rats. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-28T14:05:07Z 2017-11-28T14:05:07Z 2017-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ARAÚJO, A. A. de et al. Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One, v. 12, p. 1-21, aug. 2017. 1932-6203 (Online) http://www.repositorio.ufc.br/handle/riufc/28053 |
identifier_str_mv |
ARAÚJO, A. A. de et al. Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One, v. 12, p. 1-21, aug. 2017. 1932-6203 (Online) |
url |
http://www.repositorio.ufc.br/handle/riufc/28053 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLoS One |
publisher.none.fl_str_mv |
PLoS One |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
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1813028799382552576 |