Virulence properties of a peptide hemolysin produced by Enterococcus faecalis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
| Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/5707 |
Resumo: | FURUMURA, M. T. ; FIGUEIRÊDO, P. M. S. ; CARBONELL, G. V. ; DARINI, A. L. ; BONFIM, Maria Rosa Quaresma ; TURRI, Rosimary de Jesus Gomes ; MORAES, Francyelle Costa ; ANDRADE, Cristina Monteiro de ; MENDES, Hermínio Benitez Rabello ; MONTEIRO NETO, Valério ; YANO, T. Virulence properties of a peptide hemolysin produced by Enterococcus faecalis. Advances in Bioscience and Biotechnology, v. 03, n. 7, p. 909-917, nov. 2012. |
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Virulence properties of a peptide hemolysin produced by Enterococcus faecalisEnterococcus faecalisCélulas EpiteliaisApoptoseFURUMURA, M. T. ; FIGUEIRÊDO, P. M. S. ; CARBONELL, G. V. ; DARINI, A. L. ; BONFIM, Maria Rosa Quaresma ; TURRI, Rosimary de Jesus Gomes ; MORAES, Francyelle Costa ; ANDRADE, Cristina Monteiro de ; MENDES, Hermínio Benitez Rabello ; MONTEIRO NETO, Valério ; YANO, T. Virulence properties of a peptide hemolysin produced by Enterococcus faecalis. Advances in Bioscience and Biotechnology, v. 03, n. 7, p. 909-917, nov. 2012.The characterization and prevalence of virulence fac- tors associated with enterococcal invasiveness and severity of disease are important areas to be investi- gated. Recently, we described the production of a heat- stable hemolysin by clinical isolates of Enterococcus faecalis cultived in BHI-GA (BHI with glucose and L- arginine). Now, we purified the hemolysin from the cul- ture supernatant by ultrafiltration (PM-10 membrane) and ethanol extraction followed by chromatography in a Bondapak C18 and Superdex Peptide columns. The hemolytic activity was not affected by the proteo- lytic enzymes. Cholesterol, phospholipids, EDTA and also bivalent ions did not inhibit the hemolytic activity. Among the various carbohydrates, only dextran 4 protected the erythrocytes against lyse. Scanning elec- tron microscopy showed that lyse of erythrocytes oc-cured at once after the exposure to the hemolysin. The mitochondrial activity and the cell membrane integrity were significantly affected by the hemolysis, within 20 min of exposure and caused apoptosis after 12 h incubation, 51.92% in HeLa and 68% in HEp-2 cells, analyzed by flow cytometry. These results sug- gest that the heat-stable pore forming hemolysin might be a putative virulence factor in enterococci in- fections.2013-08-30T12:04:44Z2013-08-30T12:04:44Z2012-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFURUMURA, M. T. ; FIGUEIRÊDO, P. M. S. ; CARBONELL, G. V. ; DARINI, A. L. ; BONFIM, M. R. Q. ; TURRI, R. J. G. ; MORAES, F. C. ; ANDRADE, C. M. ; MENDES, H. B. R. ; MONTEIRO NETO, V. ; YANO, T. (2012)2156-8456http://www.repositorio.ufc.br/handle/riufc/5707Furumura, M. T.Figueirêdo, Patricia de Maria SilvaCarbonell, Gleize V.Darini, Ana Lucia da CostaBomfim, Maria Rosa QuaresmaTurri, Rosimary de Jesus GomesMoraes, Francyelle CostaAndrade, Cristina Monteiro deMendes, Hermínio Benitez RabeloMonteiro Neto, Valérioengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-11-10T23:32:24Zoai:repositorio.ufc.br:riufc/5707Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:15:51.022330Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
| dc.title.none.fl_str_mv |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis |
| title |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis |
| spellingShingle |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis Furumura, M. T. Enterococcus faecalis Células Epiteliais Apoptose |
| title_short |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis |
| title_full |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis |
| title_fullStr |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis |
| title_full_unstemmed |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis |
| title_sort |
Virulence properties of a peptide hemolysin produced by Enterococcus faecalis |
| author |
Furumura, M. T. |
| author_facet |
Furumura, M. T. Figueirêdo, Patricia de Maria Silva Carbonell, Gleize V. Darini, Ana Lucia da Costa Bomfim, Maria Rosa Quaresma Turri, Rosimary de Jesus Gomes Moraes, Francyelle Costa Andrade, Cristina Monteiro de Mendes, Hermínio Benitez Rabelo Monteiro Neto, Valério |
| author_role |
author |
| author2 |
Figueirêdo, Patricia de Maria Silva Carbonell, Gleize V. Darini, Ana Lucia da Costa Bomfim, Maria Rosa Quaresma Turri, Rosimary de Jesus Gomes Moraes, Francyelle Costa Andrade, Cristina Monteiro de Mendes, Hermínio Benitez Rabelo Monteiro Neto, Valério |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Furumura, M. T. Figueirêdo, Patricia de Maria Silva Carbonell, Gleize V. Darini, Ana Lucia da Costa Bomfim, Maria Rosa Quaresma Turri, Rosimary de Jesus Gomes Moraes, Francyelle Costa Andrade, Cristina Monteiro de Mendes, Hermínio Benitez Rabelo Monteiro Neto, Valério |
| dc.subject.por.fl_str_mv |
Enterococcus faecalis Células Epiteliais Apoptose |
| topic |
Enterococcus faecalis Células Epiteliais Apoptose |
| description |
FURUMURA, M. T. ; FIGUEIRÊDO, P. M. S. ; CARBONELL, G. V. ; DARINI, A. L. ; BONFIM, Maria Rosa Quaresma ; TURRI, Rosimary de Jesus Gomes ; MORAES, Francyelle Costa ; ANDRADE, Cristina Monteiro de ; MENDES, Hermínio Benitez Rabello ; MONTEIRO NETO, Valério ; YANO, T. Virulence properties of a peptide hemolysin produced by Enterococcus faecalis. Advances in Bioscience and Biotechnology, v. 03, n. 7, p. 909-917, nov. 2012. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-11 2013-08-30T12:04:44Z 2013-08-30T12:04:44Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
FURUMURA, M. T. ; FIGUEIRÊDO, P. M. S. ; CARBONELL, G. V. ; DARINI, A. L. ; BONFIM, M. R. Q. ; TURRI, R. J. G. ; MORAES, F. C. ; ANDRADE, C. M. ; MENDES, H. B. R. ; MONTEIRO NETO, V. ; YANO, T. (2012) 2156-8456 http://www.repositorio.ufc.br/handle/riufc/5707 |
| identifier_str_mv |
FURUMURA, M. T. ; FIGUEIRÊDO, P. M. S. ; CARBONELL, G. V. ; DARINI, A. L. ; BONFIM, M. R. Q. ; TURRI, R. J. G. ; MORAES, F. C. ; ANDRADE, C. M. ; MENDES, H. B. R. ; MONTEIRO NETO, V. ; YANO, T. (2012) 2156-8456 |
| url |
http://www.repositorio.ufc.br/handle/riufc/5707 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
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Universidade Federal do Ceará (UFC) |
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UFC |
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UFC |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
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bu@ufc.br || repositorio@ufc.br |
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1813028728362500096 |