Efeito do anestésico eugenol na qualidade espermática do sêmen de tilápia do Nilo (Oreochromis niloticus)

• Autor(a) principal: Pereira, Paula Naiane Braga
• Data de Publicação: 2016
• Outros Autores: Silva, Angelita Costa da, Teixeira, Erivânia Gomes, Farias, Wladimir Ronald Lobo
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/20544
• topic: Reprodução animal; Eugenol; Espermatozóides - peixes; Aquicultura

The use of high quality gametes of fish breeding is a vital factor to ensure excellence in the production of offspring for aquaculture. In this work was to evaluate the influence of natural anesthetic eugenol on semen quality of Nile tilapia (Oreochromis niloticus), through the determination of sperm motility rate, pH, semen volume produced and motility of sperm from semen samples collected from fish (n = 10) anesthetized with 120 mg L - 1 of eugenol, as well as of animals subjected to the anesthetic. In addition, the influence of the anesthetic in the morphology of male gametes was also assessed through observation of 150 sperm of each fish, in both treatments (n = 8), the feasibility of using this anesthetic agent in experimental procedures. The average sperm motility rate of semen non - anesthetized fish was significantly higher (91 ±%) observed in 9944 material collected from anesthetized specimens from 81.5 ± 7835 percent, revealing that the rate of sperm motility decreased with the use of the anesthetic. The variation of pH 8.4 ± 0699 was in control and treatment 8.3 ± 0, 483 in fish that passed through anesthetic induction, so there is no statistically significant difference between the two groups. There were no differences between the treatment and control group of anesthetized fish regarding the volume of semen produced from 310 ± 137 μL and 260 ± 142 μL, respectively. The animals in the control group showed an average sperm motility of 588.9 ± 320 seconds, while for the sperm of anesthetized fish this averaging was 534.7 ± 310 seconds, but there was in the tatistically significant variation between the two treatments. Morphology sperm tests, there were statistically significant differences between the two groups: fish from the control group showed an average of 3.0 ± 117.50 sperm without any deformity, while the other group the sperm with the absence of deformities were 88 ± 2.08. So the anesthetic eugenol is not indicated for use in breeding, because according to the data obtained there is prejudice in the production of male gametes.