MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/5380 |
Resumo: | Abstract MTHFR C677T and Helicobacter pylori infec- tion are believed to play critical roles in the DNA methylation process, an epigenetic feature frequently found in gastric cancer. The aim of this study was to verify the associations between the MTHFR C677T polymorphism and the methylation status of three gastric cancer-related genes. The influence of H. pylori strains was also assessed. DNA extracted from 71 gastric tumor samples was available for MTHFR C677T genotyping by PCR-RFLP, promoter methylation identification by MS-PCR and H. pylori detection and posterior subtyping ( cag A and vac A genes) by PCR. In the distal tumors, a positive correlation was found between the methylation of CDKN2A and the allele T carriers ( r =0.357; p =0.009). Considering the eldest patients (age ≥ 60 years old), this correlation was even higher ( r =0,417; p =0.014). H. pylori infection by highly pathogenic strains ( cag A+/ vac As1m1) was also found correlated to promoter methylation of CDKN2A and the allele T carriers in distal tumors ( r =0.484; p =0.026). No significant correlatio n was verified between MTHFR C677T genotype and promoter methylation status when we analyzed the general sample. DNA methylation in CDKN2A associated to the MTHFR 677T carrier is suggested to be a distal tumor characteristic, especially in those 60 years old or older, and it seems to depend on the infection by H. pylori cag A/ vac As1m1 strains |
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Repositório Institucional da Universidade Federal do Ceará (UFC) |
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MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infectionHelicobacter pyloriNeoplasias GástricasAbstract MTHFR C677T and Helicobacter pylori infec- tion are believed to play critical roles in the DNA methylation process, an epigenetic feature frequently found in gastric cancer. The aim of this study was to verify the associations between the MTHFR C677T polymorphism and the methylation status of three gastric cancer-related genes. The influence of H. pylori strains was also assessed. DNA extracted from 71 gastric tumor samples was available for MTHFR C677T genotyping by PCR-RFLP, promoter methylation identification by MS-PCR and H. pylori detection and posterior subtyping ( cag A and vac A genes) by PCR. In the distal tumors, a positive correlation was found between the methylation of CDKN2A and the allele T carriers ( r =0.357; p =0.009). Considering the eldest patients (age ≥ 60 years old), this correlation was even higher ( r =0,417; p =0.014). H. pylori infection by highly pathogenic strains ( cag A+/ vac As1m1) was also found correlated to promoter methylation of CDKN2A and the allele T carriers in distal tumors ( r =0.484; p =0.026). No significant correlatio n was verified between MTHFR C677T genotype and promoter methylation status when we analyzed the general sample. DNA methylation in CDKN2A associated to the MTHFR 677T carrier is suggested to be a distal tumor characteristic, especially in those 60 years old or older, and it seems to depend on the infection by H. pylori cag A/ vac As1m1 strainsVirchows Archiv2013-07-16T14:19:09Z2013-07-16T14:19:09Z2010-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNEVES FILHO, E. H. C. et al. MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection. Virchows Archiv, Berlin, v. 457, n. 6, p. 627-633, dez. 2010.1432-2307 Online0945-6317 Impressahttp://www.repositorio.ufc.br/handle/riufc/5380Neves Filho, Eduardo Henrique CunhaAlves, Markênia Kélia SantosLima, Valeska PortelaRabenhorst, Silvia Helena Baremengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-12-15T18:16:12Zoai:repositorio.ufc.br:riufc/5380Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:33:15.112442Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection |
title |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection |
spellingShingle |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection Neves Filho, Eduardo Henrique Cunha Helicobacter pylori Neoplasias Gástricas |
title_short |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection |
title_full |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection |
title_fullStr |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection |
title_full_unstemmed |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection |
title_sort |
MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection |
author |
Neves Filho, Eduardo Henrique Cunha |
author_facet |
Neves Filho, Eduardo Henrique Cunha Alves, Markênia Kélia Santos Lima, Valeska Portela Rabenhorst, Silvia Helena Barem |
author_role |
author |
author2 |
Alves, Markênia Kélia Santos Lima, Valeska Portela Rabenhorst, Silvia Helena Barem |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Neves Filho, Eduardo Henrique Cunha Alves, Markênia Kélia Santos Lima, Valeska Portela Rabenhorst, Silvia Helena Barem |
dc.subject.por.fl_str_mv |
Helicobacter pylori Neoplasias Gástricas |
topic |
Helicobacter pylori Neoplasias Gástricas |
description |
Abstract MTHFR C677T and Helicobacter pylori infec- tion are believed to play critical roles in the DNA methylation process, an epigenetic feature frequently found in gastric cancer. The aim of this study was to verify the associations between the MTHFR C677T polymorphism and the methylation status of three gastric cancer-related genes. The influence of H. pylori strains was also assessed. DNA extracted from 71 gastric tumor samples was available for MTHFR C677T genotyping by PCR-RFLP, promoter methylation identification by MS-PCR and H. pylori detection and posterior subtyping ( cag A and vac A genes) by PCR. In the distal tumors, a positive correlation was found between the methylation of CDKN2A and the allele T carriers ( r =0.357; p =0.009). Considering the eldest patients (age ≥ 60 years old), this correlation was even higher ( r =0,417; p =0.014). H. pylori infection by highly pathogenic strains ( cag A+/ vac As1m1) was also found correlated to promoter methylation of CDKN2A and the allele T carriers in distal tumors ( r =0.484; p =0.026). No significant correlatio n was verified between MTHFR C677T genotype and promoter methylation status when we analyzed the general sample. DNA methylation in CDKN2A associated to the MTHFR 677T carrier is suggested to be a distal tumor characteristic, especially in those 60 years old or older, and it seems to depend on the infection by H. pylori cag A/ vac As1m1 strains |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12 2013-07-16T14:19:09Z 2013-07-16T14:19:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
NEVES FILHO, E. H. C. et al. MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection. Virchows Archiv, Berlin, v. 457, n. 6, p. 627-633, dez. 2010. 1432-2307 Online 0945-6317 Impressa http://www.repositorio.ufc.br/handle/riufc/5380 |
identifier_str_mv |
NEVES FILHO, E. H. C. et al. MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection. Virchows Archiv, Berlin, v. 457, n. 6, p. 627-633, dez. 2010. 1432-2307 Online 0945-6317 Impressa |
url |
http://www.repositorio.ufc.br/handle/riufc/5380 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Virchows Archiv |
publisher.none.fl_str_mv |
Virchows Archiv |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1813028851965493248 |