MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection

Detalhes bibliográficos
Autor(a) principal: Neves Filho, Eduardo Henrique Cunha
Data de Publicação: 2010
Outros Autores: Alves, Markênia Kélia Santos, Lima, Valeska Portela, Rabenhorst, Silvia Helena Barem
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/5380
Resumo: Abstract MTHFR C677T and Helicobacter pylori infec- tion are believed to play critical roles in the DNA methylation process, an epigenetic feature frequently found in gastric cancer. The aim of this study was to verify the associations between the MTHFR C677T polymorphism and the methylation status of three gastric cancer-related genes. The influence of H. pylori strains was also assessed. DNA extracted from 71 gastric tumor samples was available for MTHFR C677T genotyping by PCR-RFLP, promoter methylation identification by MS-PCR and H. pylori detection and posterior subtyping ( cag A and vac A genes) by PCR. In the distal tumors, a positive correlation was found between the methylation of CDKN2A and the allele T carriers ( r =0.357; p =0.009). Considering the eldest patients (age ≥ 60 years old), this correlation was even higher ( r =0,417; p =0.014). H. pylori infection by highly pathogenic strains ( cag A+/ vac As1m1) was also found correlated to promoter methylation of CDKN2A and the allele T carriers in distal tumors ( r =0.484; p =0.026). No significant correlatio n was verified between MTHFR C677T genotype and promoter methylation status when we analyzed the general sample. DNA methylation in CDKN2A associated to the MTHFR 677T carrier is suggested to be a distal tumor characteristic, especially in those 60 years old or older, and it seems to depend on the infection by H. pylori cag A/ vac As1m1 strains
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spelling MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infectionHelicobacter pyloriNeoplasias GástricasAbstract MTHFR C677T and Helicobacter pylori infec- tion are believed to play critical roles in the DNA methylation process, an epigenetic feature frequently found in gastric cancer. The aim of this study was to verify the associations between the MTHFR C677T polymorphism and the methylation status of three gastric cancer-related genes. The influence of H. pylori strains was also assessed. DNA extracted from 71 gastric tumor samples was available for MTHFR C677T genotyping by PCR-RFLP, promoter methylation identification by MS-PCR and H. pylori detection and posterior subtyping ( cag A and vac A genes) by PCR. In the distal tumors, a positive correlation was found between the methylation of CDKN2A and the allele T carriers ( r =0.357; p =0.009). Considering the eldest patients (age ≥ 60 years old), this correlation was even higher ( r =0,417; p =0.014). H. pylori infection by highly pathogenic strains ( cag A+/ vac As1m1) was also found correlated to promoter methylation of CDKN2A and the allele T carriers in distal tumors ( r =0.484; p =0.026). No significant correlatio n was verified between MTHFR C677T genotype and promoter methylation status when we analyzed the general sample. DNA methylation in CDKN2A associated to the MTHFR 677T carrier is suggested to be a distal tumor characteristic, especially in those 60 years old or older, and it seems to depend on the infection by H. pylori cag A/ vac As1m1 strainsVirchows Archiv2013-07-16T14:19:09Z2013-07-16T14:19:09Z2010-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNEVES FILHO, E. H. C. et al. MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection. Virchows Archiv, Berlin, v. 457, n. 6, p. 627-633, dez. 2010.1432-2307 Online0945-6317 Impressahttp://www.repositorio.ufc.br/handle/riufc/5380Neves Filho, Eduardo Henrique CunhaAlves, Markênia Kélia SantosLima, Valeska PortelaRabenhorst, Silvia Helena Baremengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2021-12-15T18:16:12Zoai:repositorio.ufc.br:riufc/5380Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:33:15.112442Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
title MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
spellingShingle MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
Neves Filho, Eduardo Henrique Cunha
Helicobacter pylori
Neoplasias Gástricas
title_short MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
title_full MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
title_fullStr MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
title_full_unstemmed MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
title_sort MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection
author Neves Filho, Eduardo Henrique Cunha
author_facet Neves Filho, Eduardo Henrique Cunha
Alves, Markênia Kélia Santos
Lima, Valeska Portela
Rabenhorst, Silvia Helena Barem
author_role author
author2 Alves, Markênia Kélia Santos
Lima, Valeska Portela
Rabenhorst, Silvia Helena Barem
author2_role author
author
author
dc.contributor.author.fl_str_mv Neves Filho, Eduardo Henrique Cunha
Alves, Markênia Kélia Santos
Lima, Valeska Portela
Rabenhorst, Silvia Helena Barem
dc.subject.por.fl_str_mv Helicobacter pylori
Neoplasias Gástricas
topic Helicobacter pylori
Neoplasias Gástricas
description Abstract MTHFR C677T and Helicobacter pylori infec- tion are believed to play critical roles in the DNA methylation process, an epigenetic feature frequently found in gastric cancer. The aim of this study was to verify the associations between the MTHFR C677T polymorphism and the methylation status of three gastric cancer-related genes. The influence of H. pylori strains was also assessed. DNA extracted from 71 gastric tumor samples was available for MTHFR C677T genotyping by PCR-RFLP, promoter methylation identification by MS-PCR and H. pylori detection and posterior subtyping ( cag A and vac A genes) by PCR. In the distal tumors, a positive correlation was found between the methylation of CDKN2A and the allele T carriers ( r =0.357; p =0.009). Considering the eldest patients (age ≥ 60 years old), this correlation was even higher ( r =0,417; p =0.014). H. pylori infection by highly pathogenic strains ( cag A+/ vac As1m1) was also found correlated to promoter methylation of CDKN2A and the allele T carriers in distal tumors ( r =0.484; p =0.026). No significant correlatio n was verified between MTHFR C677T genotype and promoter methylation status when we analyzed the general sample. DNA methylation in CDKN2A associated to the MTHFR 677T carrier is suggested to be a distal tumor characteristic, especially in those 60 years old or older, and it seems to depend on the infection by H. pylori cag A/ vac As1m1 strains
publishDate 2010
dc.date.none.fl_str_mv 2010-12
2013-07-16T14:19:09Z
2013-07-16T14:19:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv NEVES FILHO, E. H. C. et al. MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection. Virchows Archiv, Berlin, v. 457, n. 6, p. 627-633, dez. 2010.
1432-2307 Online
0945-6317 Impressa
http://www.repositorio.ufc.br/handle/riufc/5380
identifier_str_mv NEVES FILHO, E. H. C. et al. MTHFR C677T polymorphism and differential methylation status in gastric cancer : an association with Helicobacter pylori infection. Virchows Archiv, Berlin, v. 457, n. 6, p. 627-633, dez. 2010.
1432-2307 Online
0945-6317 Impressa
url http://www.repositorio.ufc.br/handle/riufc/5380
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Virchows Archiv
publisher.none.fl_str_mv Virchows Archiv
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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