Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/10996 |
Resumo: | The objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with Enterococcus faecalis. Forty-five human lower premolars were infected with a culture of E. faecalis (ATCC 29212) for 28 days. Five other teeth that were neither contaminated nor instrumented were used as controls. The 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (K-type, Dentsply Maillefer, Ballaigues, Switzerland); rotary (MTwo, VDW GmbH, Munich, Germany); and reciprocating (Reciproc R50, VDW GmbH, Munich, Germany) instruments. During chemomechanical preparation, 21 mL of 2.5% NaOCl was used as the irrigating solution. Microbiological sampling was performed before (S1) and immediately after (S2) the chemomechanical preparation using sterilized paper points. Specimens were then cleaved, and 0.02 g of dentine chips was collected from the root thirds to verify the presence of microorganisms in dentinal tubules. All three preparation techniques reduced the number of microorganisms in the root canal lumen and dentine chips from the root thirds, but no significant differences were observed between the three groups (p > 0.05). Reciprocating instrumentation with Reciproc R50 was effective in reducing the number of microorganisms within the root canal system. Although this technique involves the use of only one file to perform the root canal therapy, it is as effective as conventional rotary instrumentation in reducing the E. faecalis biofilm from the root canal system. However, further clinical investigations are warranted in order to ratify these results. |
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Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canalsEndodontiaInstrumentos OdontológicosThe objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with Enterococcus faecalis. Forty-five human lower premolars were infected with a culture of E. faecalis (ATCC 29212) for 28 days. Five other teeth that were neither contaminated nor instrumented were used as controls. The 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (K-type, Dentsply Maillefer, Ballaigues, Switzerland); rotary (MTwo, VDW GmbH, Munich, Germany); and reciprocating (Reciproc R50, VDW GmbH, Munich, Germany) instruments. During chemomechanical preparation, 21 mL of 2.5% NaOCl was used as the irrigating solution. Microbiological sampling was performed before (S1) and immediately after (S2) the chemomechanical preparation using sterilized paper points. Specimens were then cleaved, and 0.02 g of dentine chips was collected from the root thirds to verify the presence of microorganisms in dentinal tubules. All three preparation techniques reduced the number of microorganisms in the root canal lumen and dentine chips from the root thirds, but no significant differences were observed between the three groups (p > 0.05). Reciprocating instrumentation with Reciproc R50 was effective in reducing the number of microorganisms within the root canal system. Although this technique involves the use of only one file to perform the root canal therapy, it is as effective as conventional rotary instrumentation in reducing the E. faecalis biofilm from the root canal system. However, further clinical investigations are warranted in order to ratify these results.Brazilian Oral Research2015-03-20T12:15:02Z2015-03-20T12:15:02Z2015-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfNAKAMURA, V. C. ; CANDEIRO, G. T. M. ; CAI, S. ; GAVINI, G. Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals. Brazilian Oral Research, v. 29, n. 1, p. 1-7, jan. 2015.1806-8324http://www.repositorio.ufc.br/handle/riufc/10996Nakamura, Vitor CesarCandeiro, George Taccio de MirandaCai, SilvanaGavini, Giulioengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-01-31T13:17:45Zoai:repositorio.ufc.br:riufc/10996Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2019-01-31T13:17:45Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals |
title |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals |
spellingShingle |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals Nakamura, Vitor Cesar Endodontia Instrumentos Odontológicos |
title_short |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals |
title_full |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals |
title_fullStr |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals |
title_full_unstemmed |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals |
title_sort |
Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals |
author |
Nakamura, Vitor Cesar |
author_facet |
Nakamura, Vitor Cesar Candeiro, George Taccio de Miranda Cai, Silvana Gavini, Giulio |
author_role |
author |
author2 |
Candeiro, George Taccio de Miranda Cai, Silvana Gavini, Giulio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nakamura, Vitor Cesar Candeiro, George Taccio de Miranda Cai, Silvana Gavini, Giulio |
dc.subject.por.fl_str_mv |
Endodontia Instrumentos Odontológicos |
topic |
Endodontia Instrumentos Odontológicos |
description |
The objective of the present study was to assess the effectiveness of reciprocating instrumentation in disinfecting oval-shaped root canals infected with Enterococcus faecalis. Forty-five human lower premolars were infected with a culture of E. faecalis (ATCC 29212) for 28 days. Five other teeth that were neither contaminated nor instrumented were used as controls. The 45 specimens were divided into three experimental groups (n = 15) based on the root canal preparation technique used: manual (K-type, Dentsply Maillefer, Ballaigues, Switzerland); rotary (MTwo, VDW GmbH, Munich, Germany); and reciprocating (Reciproc R50, VDW GmbH, Munich, Germany) instruments. During chemomechanical preparation, 21 mL of 2.5% NaOCl was used as the irrigating solution. Microbiological sampling was performed before (S1) and immediately after (S2) the chemomechanical preparation using sterilized paper points. Specimens were then cleaved, and 0.02 g of dentine chips was collected from the root thirds to verify the presence of microorganisms in dentinal tubules. All three preparation techniques reduced the number of microorganisms in the root canal lumen and dentine chips from the root thirds, but no significant differences were observed between the three groups (p > 0.05). Reciprocating instrumentation with Reciproc R50 was effective in reducing the number of microorganisms within the root canal system. Although this technique involves the use of only one file to perform the root canal therapy, it is as effective as conventional rotary instrumentation in reducing the E. faecalis biofilm from the root canal system. However, further clinical investigations are warranted in order to ratify these results. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-20T12:15:02Z 2015-03-20T12:15:02Z 2015-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
NAKAMURA, V. C. ; CANDEIRO, G. T. M. ; CAI, S. ; GAVINI, G. Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals. Brazilian Oral Research, v. 29, n. 1, p. 1-7, jan. 2015. 1806-8324 http://www.repositorio.ufc.br/handle/riufc/10996 |
identifier_str_mv |
NAKAMURA, V. C. ; CANDEIRO, G. T. M. ; CAI, S. ; GAVINI, G. Ex vivo evaluation of three instrumentation techniques on E. faecalis biofilm within oval shaped root canals. Brazilian Oral Research, v. 29, n. 1, p. 1-7, jan. 2015. 1806-8324 |
url |
http://www.repositorio.ufc.br/handle/riufc/10996 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Oral Research |
publisher.none.fl_str_mv |
Brazilian Oral Research |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1809935800233623552 |