Detecção de betalactamases de espectro expandindo (ESBL) em cepas de coliformes isolados de hortaliças minimante comercializadas na cidade de Fortaleza -CE

• Autor(a) principal: Cunha, Francisco Afrânio
• Data de Publicação: 2007
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/17463
• topic: Ciência e tecnologia de alimentos; Hortaliças minimamente processadas; Enterobacteriaceae; Resistência a antimicrobianos; Minimally processed vegetables; Enterobacteriaceae; Antibiotic resistance; ESBL

Minimally processed vegetables (MPV) normally consist of fresh raw vegetables, washed, peeled, cut, disinfected, centrifugal dryed packed and kept under refrigeration. Vegetables are potential vehicle of microorganisms that can be associated to the outbreaks of foodborne. Countless are the causes for the presence high microbial load in that product type, among which are: the cultivation techniques, storage, transport and distribution for consumption, the practice of the use of organic fertilizer, the use of polluted water for irrigation, the transport accomplished in open crates and the hygiene conditions in the handling and preparation of meals, mainly when such foods are consumed raw. The objective of this study was to determine the presence of extendedspectrum β-betalactamases (ESBL) in strains of Enterobacteriaceae isolated of MPV marketed in the city of Fortaleza and to verify if the analyzed vegetables assists the Brazilian Food Sanitation Standard, RDC N° 12, 02 of january 2001. 80 samples of MPV were collected marketed in Fortaleza. Were collected vegetables studied: 8 samples beet; 10 lettuce; 8 of cherry-colored tomato; 8 purple cabbage, green cabbage; 10 carrot and green bean; 8 grated carrot; 10 grated carrot, green cabbage and purple cabbage; 10 salads; 8 cilantro, onion and parsley pricked. The analyses were accomplished of april from 2006 to may of 2007. The countings of mesophiles aerobic, varied from 5,60 to 13,35 log UFC / g of MPV. The countings of moulds and yeasts, varied from 5,54 to 9,88 log UFC / g MPV. The counting of total coliforms and fecal coliforms were quite high. The main isolated and identified mold was the Penicillium spp. All of the beet samples, grated carrot, carrot and green bean, grated carrot, purple cabbage and green cabbage, cilantro and parsley pricked met inappropriate for the consumption, because they presented amounts superior of 100 fecal coliforms / g samples. Of the samples of lettuce 50% they presented fecal coliforms counting above legislation. Of the tomatoes cherry analyzed 75% were inside patterns of the Brazilian legislation, for fecal coliforms. Of the samples of purple cabbage, green cabbage 87% were with fecal coliforms counting above. Of the samples of salad 80% were out of the patterns. Salmonellas were not detected in the samples of MPV analyzed. The main isolated Enterobacteriaceae were E. coli, K. pneumoniae and E. aerogenes. All of the strains of K. pneumoniae and E. aerogenes were resistant to the ampicillin. Strains of E. coli, K pneumoniae and E. aerogenes with multidrug resistance were detected. Resistance to ciprofloxacin, ceftazidime and imipenem was not observed. Enterobacteriaceae samples producing of ESBL were not detected. Were not identified strains of E. coli O15:H7 among the strains of E. coli isolated of MPV. The hygienicsanitary conditions of those products can be improved with the application of Good Practices in the whole productive chain.