Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/14115 |
Resumo: | Biofilms are microbial communities that are irreversibly attached to a surface and are embedded in a polymeric matrix produced by them. Biofilms are commonly related to nosocomial infections that showing an enhanced resistance to antimicrobial agents compared to planktonic cells, thus hindering their treatments and limiting therapeutic options. In this context, the search for new molecules with antimicrobial and antibiofilm action has become an active area of research. The plants are sources of a variety of molecules with antimicrobial properties, among them we can mention the lectins and secondary metabolites. Several studies have reported the antimicrobial and antibiofilm action of these molecules classes as an alternative to antibiotics. Thus, the aim of this study was to evaluate the antimicrobial and antibiofilm of various lectins isolated from leguminous and algae, as well as two secondary metabolites isolated from plants , derriotusone A (isolated from Lonchocarpus obtusus) and casbane diterpene (isolated from Croton nepetaefolius). The results showed that some lectins tested were able to inhibit the planktonic growth and/or the biofilm formation of certain microorganisms. The lectin isolated from Vaitarea macrocarpa (VML) showed to be the most promising lectin, showing strong action on the planktonic growth and biofilm formation of Staphylococcus aures and Staphylococcus epidermidis. The derriobstusone A showed potential antibacterial and antibiofilm activite on S. aureus, whereas Escherichia coli showed lower sensitivity to the compound. In addition, derriotusone showed a potential antioxidant activity. Regarding to casbane diterpene, in general the compound was able to inhibit planktonic growth, formation of biofilms and disrupt the preformed biofilms of the S.aures, S. epidermidis, Candida albicans and Candida glabrata, and also showed to be effective against biofilms formed by the association between these bacteria and yeasts. In conclusion, the results showed that some lectins, as the secondary metabolites used in this study, may be considered as potential antimicrobial and antibiofilm agents, thus suggesting the use of these molecules in the treatment of infections associated with different microorganisms. |
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Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínicoActivity of lectins and bioactive metabolites from plants on microbial biofilms of clinical interestBiofilmeLectinasBiomoléculasBiofilms are microbial communities that are irreversibly attached to a surface and are embedded in a polymeric matrix produced by them. Biofilms are commonly related to nosocomial infections that showing an enhanced resistance to antimicrobial agents compared to planktonic cells, thus hindering their treatments and limiting therapeutic options. In this context, the search for new molecules with antimicrobial and antibiofilm action has become an active area of research. The plants are sources of a variety of molecules with antimicrobial properties, among them we can mention the lectins and secondary metabolites. Several studies have reported the antimicrobial and antibiofilm action of these molecules classes as an alternative to antibiotics. Thus, the aim of this study was to evaluate the antimicrobial and antibiofilm of various lectins isolated from leguminous and algae, as well as two secondary metabolites isolated from plants , derriotusone A (isolated from Lonchocarpus obtusus) and casbane diterpene (isolated from Croton nepetaefolius). The results showed that some lectins tested were able to inhibit the planktonic growth and/or the biofilm formation of certain microorganisms. The lectin isolated from Vaitarea macrocarpa (VML) showed to be the most promising lectin, showing strong action on the planktonic growth and biofilm formation of Staphylococcus aures and Staphylococcus epidermidis. The derriobstusone A showed potential antibacterial and antibiofilm activite on S. aureus, whereas Escherichia coli showed lower sensitivity to the compound. In addition, derriotusone showed a potential antioxidant activity. Regarding to casbane diterpene, in general the compound was able to inhibit planktonic growth, formation of biofilms and disrupt the preformed biofilms of the S.aures, S. epidermidis, Candida albicans and Candida glabrata, and also showed to be effective against biofilms formed by the association between these bacteria and yeasts. In conclusion, the results showed that some lectins, as the secondary metabolites used in this study, may be considered as potential antimicrobial and antibiofilm agents, thus suggesting the use of these molecules in the treatment of infections associated with different microorganisms.Biofilmes são comunidades microbianas que encontram-se irreversivelmente associadas a uma superfície e estão inseridas em uma matriz polimérica produzida por elas mesmas. Os biofilmes estão comumente relacionados a infecções nosocomiais que apresentam maior resistência a agentes antimicrobianos quando comparadas com células planctônicas, dificultando assim seus tratamentos e limitando as opções terapêuticas. Nesse sentido a busca por novas moléculas com ação antimicrobiana e antibiofilme tornou-se uma área ativa na pesquisa científica. Os vegetais são fontes de uma variedade de moléculas com propriedades antimicrobianas, dentre estas podemos citar as lectinas e os metabólitos secundários. Diversos estudos têm relatado a ação antimicrobiana e antibiofilme dessas classes de moléculas como forma alternativa ao uso de antibióticos. Assim, o objetivo desse trabalho foi avaliar a ação antimicrobiana e antibiofilme de diversas lectinas isoladas de leguminosas e algas, bem como de dois metabólitos secundários isolados de plantas, derriotusona A (isolado de Lonchocarpus obtusus) e diterpeno casbano (isolado de Croton nepetaefolius). Os resultados demonstraram que algumas das lectinas testadas foram capazes de inibir o crescimento planctônico e/ou a formação de biofilme de determinados micro-organismos. A lectina isolada de Vaitarea macrocarpa (VML) mostrou ser a lectina mais promissora, mostrando forte ação sobre o crescimento planctônico e formação de biofilmes de Staphylococcus aures e Staphylococcus epidermidis. Derriobstusona A mostrou uma potencial ação antibacteriana e antibiofilme sobre S. aureus, enquanto que, Escherichia coli apresentou menor sensibilidade ao composto. Em adição, derriotusona A demonstrou uma potencial ação antioxidante. Em relação ao diterpeno casbano, em geral o composto foi capaz de inibir o crescimento planctônico, formação de biofilmes e causar danos nos biofilmes pré-formados de S.aures, S. epidermidis, Candida albicans e Candida glabrata, e mostrou ainda ser efetivo contra biofilmes formados pela associação entre estas bactérias e leveduras. Em conclusão, os resultados mostraram que algumas lectinas, assim como os metabólitos secundários utilizados nesse estudo, podem ser consideradas potenciais agentes antimicrobianas e antibiofilmes, sugerindo assim o uso dessas moléculas no tratamento de infecções associadas a diferentes micro-organismos.Cavada, Benildo SousaTeixeira, Edson HolandaVasconcelos, Mayron Alves de2015-11-24T20:49:45Z2015-11-24T20:49:45Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfVASCONCELOS, M. A. Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico. 2013. 266 f. Tese (Doutorado em Bioquímica) - Centro de Ciências, Universidade Federal do Ceará, Fortaleza, 2013.http://www.repositorio.ufc.br/handle/riufc/14115porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-05-03T16:11:45Zoai:repositorio.ufc.br:riufc/14115Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:32:15.075796Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico Activity of lectins and bioactive metabolites from plants on microbial biofilms of clinical interest |
title |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico |
spellingShingle |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico Vasconcelos, Mayron Alves de Biofilme Lectinas Biomoléculas |
title_short |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico |
title_full |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico |
title_fullStr |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico |
title_full_unstemmed |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico |
title_sort |
Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico |
author |
Vasconcelos, Mayron Alves de |
author_facet |
Vasconcelos, Mayron Alves de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Cavada, Benildo Sousa Teixeira, Edson Holanda |
dc.contributor.author.fl_str_mv |
Vasconcelos, Mayron Alves de |
dc.subject.por.fl_str_mv |
Biofilme Lectinas Biomoléculas |
topic |
Biofilme Lectinas Biomoléculas |
description |
Biofilms are microbial communities that are irreversibly attached to a surface and are embedded in a polymeric matrix produced by them. Biofilms are commonly related to nosocomial infections that showing an enhanced resistance to antimicrobial agents compared to planktonic cells, thus hindering their treatments and limiting therapeutic options. In this context, the search for new molecules with antimicrobial and antibiofilm action has become an active area of research. The plants are sources of a variety of molecules with antimicrobial properties, among them we can mention the lectins and secondary metabolites. Several studies have reported the antimicrobial and antibiofilm action of these molecules classes as an alternative to antibiotics. Thus, the aim of this study was to evaluate the antimicrobial and antibiofilm of various lectins isolated from leguminous and algae, as well as two secondary metabolites isolated from plants , derriotusone A (isolated from Lonchocarpus obtusus) and casbane diterpene (isolated from Croton nepetaefolius). The results showed that some lectins tested were able to inhibit the planktonic growth and/or the biofilm formation of certain microorganisms. The lectin isolated from Vaitarea macrocarpa (VML) showed to be the most promising lectin, showing strong action on the planktonic growth and biofilm formation of Staphylococcus aures and Staphylococcus epidermidis. The derriobstusone A showed potential antibacterial and antibiofilm activite on S. aureus, whereas Escherichia coli showed lower sensitivity to the compound. In addition, derriotusone showed a potential antioxidant activity. Regarding to casbane diterpene, in general the compound was able to inhibit planktonic growth, formation of biofilms and disrupt the preformed biofilms of the S.aures, S. epidermidis, Candida albicans and Candida glabrata, and also showed to be effective against biofilms formed by the association between these bacteria and yeasts. In conclusion, the results showed that some lectins, as the secondary metabolites used in this study, may be considered as potential antimicrobial and antibiofilm agents, thus suggesting the use of these molecules in the treatment of infections associated with different microorganisms. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2015-11-24T20:49:45Z 2015-11-24T20:49:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VASCONCELOS, M. A. Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico. 2013. 266 f. Tese (Doutorado em Bioquímica) - Centro de Ciências, Universidade Federal do Ceará, Fortaleza, 2013. http://www.repositorio.ufc.br/handle/riufc/14115 |
identifier_str_mv |
VASCONCELOS, M. A. Atividade de lectinas e metabólitos bioativos de plantas sobre biofilmes microbianos de interesse clínico. 2013. 266 f. Tese (Doutorado em Bioquímica) - Centro de Ciências, Universidade Federal do Ceará, Fortaleza, 2013. |
url |
http://www.repositorio.ufc.br/handle/riufc/14115 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
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