Protein adsorption onto modified porous silica by single and binary human serum protein solutions

Detalhes bibliográficos
Autor(a) principal: Gondim, Diego Romão
Data de Publicação: 2021
Outros Autores: Cecilia, Juan Antonio, Rodrigues, Thainá Nobre Barros, Vilarrasa-García, Enrique, Rodríguez-Castellón, Enrique, Azevedo, Diana Cristina Silva de, Silva Júnior, Ivanildo José da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/69562
Resumo: Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation.
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spelling Protein adsorption onto modified porous silica by single and binary human serum protein solutionsPurificationAdsorption isothermHuman serumPorous silicaSBA-15Mesocellular foamTypical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation.International Journal of Molecular Sciences2022-11-25T17:29:59Z2022-11-25T17:29:59Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAZEVEDO, D. C. S. et al. Protein adsorption onto modified porous silica by single and binary human serum protein solutions. International Journal of Molecular Sciences, [s.l.], v. 22, n. 17, 2021. DOI: https://doi.org/10.3390/ijms221791641422-0067http://www.repositorio.ufc.br/handle/riufc/69562Gondim, Diego RomãoCecilia, Juan AntonioRodrigues, Thainá Nobre BarrosVilarrasa-García, EnriqueRodríguez-Castellón, EnriqueAzevedo, Diana Cristina Silva deSilva Júnior, Ivanildo José daengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-11-25T17:43:01Zoai:repositorio.ufc.br:riufc/69562Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:36:55.251429Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Protein adsorption onto modified porous silica by single and binary human serum protein solutions
title Protein adsorption onto modified porous silica by single and binary human serum protein solutions
spellingShingle Protein adsorption onto modified porous silica by single and binary human serum protein solutions
Gondim, Diego Romão
Purification
Adsorption isotherm
Human serum
Porous silica
SBA-15
Mesocellular foam
title_short Protein adsorption onto modified porous silica by single and binary human serum protein solutions
title_full Protein adsorption onto modified porous silica by single and binary human serum protein solutions
title_fullStr Protein adsorption onto modified porous silica by single and binary human serum protein solutions
title_full_unstemmed Protein adsorption onto modified porous silica by single and binary human serum protein solutions
title_sort Protein adsorption onto modified porous silica by single and binary human serum protein solutions
author Gondim, Diego Romão
author_facet Gondim, Diego Romão
Cecilia, Juan Antonio
Rodrigues, Thainá Nobre Barros
Vilarrasa-García, Enrique
Rodríguez-Castellón, Enrique
Azevedo, Diana Cristina Silva de
Silva Júnior, Ivanildo José da
author_role author
author2 Cecilia, Juan Antonio
Rodrigues, Thainá Nobre Barros
Vilarrasa-García, Enrique
Rodríguez-Castellón, Enrique
Azevedo, Diana Cristina Silva de
Silva Júnior, Ivanildo José da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gondim, Diego Romão
Cecilia, Juan Antonio
Rodrigues, Thainá Nobre Barros
Vilarrasa-García, Enrique
Rodríguez-Castellón, Enrique
Azevedo, Diana Cristina Silva de
Silva Júnior, Ivanildo José da
dc.subject.por.fl_str_mv Purification
Adsorption isotherm
Human serum
Porous silica
SBA-15
Mesocellular foam
topic Purification
Adsorption isotherm
Human serum
Porous silica
SBA-15
Mesocellular foam
description Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022-11-25T17:29:59Z
2022-11-25T17:29:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv AZEVEDO, D. C. S. et al. Protein adsorption onto modified porous silica by single and binary human serum protein solutions. International Journal of Molecular Sciences, [s.l.], v. 22, n. 17, 2021. DOI: https://doi.org/10.3390/ijms22179164
1422-0067
http://www.repositorio.ufc.br/handle/riufc/69562
identifier_str_mv AZEVEDO, D. C. S. et al. Protein adsorption onto modified porous silica by single and binary human serum protein solutions. International Journal of Molecular Sciences, [s.l.], v. 22, n. 17, 2021. DOI: https://doi.org/10.3390/ijms22179164
1422-0067
url http://www.repositorio.ufc.br/handle/riufc/69562
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv International Journal of Molecular Sciences
publisher.none.fl_str_mv International Journal of Molecular Sciences
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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