Protein adsorption onto modified porous silica by single and binary human serum protein solutions
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/69562 |
Resumo: | Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation. |
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Protein adsorption onto modified porous silica by single and binary human serum protein solutionsPurificationAdsorption isothermHuman serumPorous silicaSBA-15Mesocellular foamTypical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation.International Journal of Molecular Sciences2022-11-25T17:29:59Z2022-11-25T17:29:59Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAZEVEDO, D. C. S. et al. Protein adsorption onto modified porous silica by single and binary human serum protein solutions. International Journal of Molecular Sciences, [s.l.], v. 22, n. 17, 2021. DOI: https://doi.org/10.3390/ijms221791641422-0067http://www.repositorio.ufc.br/handle/riufc/69562Gondim, Diego RomãoCecilia, Juan AntonioRodrigues, Thainá Nobre BarrosVilarrasa-García, EnriqueRodríguez-Castellón, EnriqueAzevedo, Diana Cristina Silva deSilva Júnior, Ivanildo José daengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-11-25T17:43:01Zoai:repositorio.ufc.br:riufc/69562Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:36:55.251429Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions |
title |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions |
spellingShingle |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions Gondim, Diego Romão Purification Adsorption isotherm Human serum Porous silica SBA-15 Mesocellular foam |
title_short |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions |
title_full |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions |
title_fullStr |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions |
title_full_unstemmed |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions |
title_sort |
Protein adsorption onto modified porous silica by single and binary human serum protein solutions |
author |
Gondim, Diego Romão |
author_facet |
Gondim, Diego Romão Cecilia, Juan Antonio Rodrigues, Thainá Nobre Barros Vilarrasa-García, Enrique Rodríguez-Castellón, Enrique Azevedo, Diana Cristina Silva de Silva Júnior, Ivanildo José da |
author_role |
author |
author2 |
Cecilia, Juan Antonio Rodrigues, Thainá Nobre Barros Vilarrasa-García, Enrique Rodríguez-Castellón, Enrique Azevedo, Diana Cristina Silva de Silva Júnior, Ivanildo José da |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gondim, Diego Romão Cecilia, Juan Antonio Rodrigues, Thainá Nobre Barros Vilarrasa-García, Enrique Rodríguez-Castellón, Enrique Azevedo, Diana Cristina Silva de Silva Júnior, Ivanildo José da |
dc.subject.por.fl_str_mv |
Purification Adsorption isotherm Human serum Porous silica SBA-15 Mesocellular foam |
topic |
Purification Adsorption isotherm Human serum Porous silica SBA-15 Mesocellular foam |
description |
Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2022-11-25T17:29:59Z 2022-11-25T17:29:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
AZEVEDO, D. C. S. et al. Protein adsorption onto modified porous silica by single and binary human serum protein solutions. International Journal of Molecular Sciences, [s.l.], v. 22, n. 17, 2021. DOI: https://doi.org/10.3390/ijms22179164 1422-0067 http://www.repositorio.ufc.br/handle/riufc/69562 |
identifier_str_mv |
AZEVEDO, D. C. S. et al. Protein adsorption onto modified porous silica by single and binary human serum protein solutions. International Journal of Molecular Sciences, [s.l.], v. 22, n. 17, 2021. DOI: https://doi.org/10.3390/ijms22179164 1422-0067 |
url |
http://www.repositorio.ufc.br/handle/riufc/69562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Journal of Molecular Sciences |
publisher.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1813028877363052544 |