Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients

Detalhes bibliográficos
Autor(a) principal: Damasceno, Lisandra Serra
Data de Publicação: 2019
Outros Autores: Vite-Garín, Tania, Ramírez, José Antonio, Rodríguez-Arellanes, Gabriela, Almeida, Marcos Abreu de, Muniz, Mauro de Medeiros, Mesquita, Jacó Ricarte Lima de, Leitão, Terezinha do Menino Jesus Silva, Taylor, Maria Lucia, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/46425
Resumo: Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.
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spelling Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patientsCoinfecçãoCoinfectionHistoplasmaHistoplasmoseHistoplasmosisHIVMixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.Revista do Instituto de Medicina Tropical de São Paulo2019-10-02T19:25:47Z2019-10-02T19:25:47Z2019-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfDAMASCENO, Lisandra Serra et al. Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients. Rev. Inst. Med. trop., São Paulo, v. 61, p. 1-10, feb. 2019.On-line 1678-9946http://www.repositorio.ufc.br/handle/riufc/46425Damasceno, Lisandra SerraVite-Garín, TaniaRamírez, José AntonioRodríguez-Arellanes, GabrielaAlmeida, Marcos Abreu deMuniz, Mauro de MedeirosMesquita, Jacó Ricarte Lima deLeitão, Terezinha do Menino Jesus SilvaTaylor, Maria LuciaZancopé-Oliveira, Rosely Mariaengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2019-10-02T19:25:47Zoai:repositorio.ufc.br:riufc/46425Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:38:51.481720Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
title Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
spellingShingle Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
Damasceno, Lisandra Serra
Coinfecção
Coinfection
Histoplasma
Histoplasmose
Histoplasmosis
HIV
title_short Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
title_full Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
title_fullStr Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
title_full_unstemmed Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
title_sort Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients
author Damasceno, Lisandra Serra
author_facet Damasceno, Lisandra Serra
Vite-Garín, Tania
Ramírez, José Antonio
Rodríguez-Arellanes, Gabriela
Almeida, Marcos Abreu de
Muniz, Mauro de Medeiros
Mesquita, Jacó Ricarte Lima de
Leitão, Terezinha do Menino Jesus Silva
Taylor, Maria Lucia
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Vite-Garín, Tania
Ramírez, José Antonio
Rodríguez-Arellanes, Gabriela
Almeida, Marcos Abreu de
Muniz, Mauro de Medeiros
Mesquita, Jacó Ricarte Lima de
Leitão, Terezinha do Menino Jesus Silva
Taylor, Maria Lucia
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Damasceno, Lisandra Serra
Vite-Garín, Tania
Ramírez, José Antonio
Rodríguez-Arellanes, Gabriela
Almeida, Marcos Abreu de
Muniz, Mauro de Medeiros
Mesquita, Jacó Ricarte Lima de
Leitão, Terezinha do Menino Jesus Silva
Taylor, Maria Lucia
Zancopé-Oliveira, Rosely Maria
dc.subject.por.fl_str_mv Coinfecção
Coinfection
Histoplasma
Histoplasmose
Histoplasmosis
HIV
topic Coinfecção
Coinfection
Histoplasma
Histoplasmose
Histoplasmosis
HIV
description Mixed infection by Histoplasma capsulatum isolates with different mating types, in AIDS-patients are described in this study. Morphological, mating type-specific PCR assay and multilocus sequencing type analysis of H. capsulatum isolates recovered from two Brazilian AIDS-patients were performed. Five H. capsulatum isolates were recovered at different times from the two patients. Three isolates were obtained from bone marrow (day 1 – CE0411) and buffy coat cultures (day 1 – CE0311; day 2 – CE0511) of patient 1, and two isolates were isolated from buffy coat cultures (day 3 – CE2813; day 12 – CE2513) of patient 2. The mycelial colonies depicted different textures and pigmentation features. Dimorphic conversion to the yeast-phase in ML-Gema medium was achieved in all isolates. MAT1-1 idiomorph was identified in CE0311, CE0411 and CE2813 isolates; MAT1-2 idiomorph was found in CE0511 and CE2513 isolates. These H. capsulatum isolates were grouped within LAm A clade, highlighting that CE0311 and CE0411 isolates formed a subgroup supported by a high bootstrap value. The CE0511, CE2513, and CE2813 isolates clustered together with a Brazilian H151 isolate. This research reports mixed infections caused by H. capsulatum isolates with different mating types in Brazilian AIDS-patients for the first time in the literature.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-02T19:25:47Z
2019-10-02T19:25:47Z
2019-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv DAMASCENO, Lisandra Serra et al. Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients. Rev. Inst. Med. trop., São Paulo, v. 61, p. 1-10, feb. 2019.
On-line 1678-9946
http://www.repositorio.ufc.br/handle/riufc/46425
identifier_str_mv DAMASCENO, Lisandra Serra et al. Mixed infection by Histoplasma capsulatum isolates with different mating types in Brazilian AIDS-patients. Rev. Inst. Med. trop., São Paulo, v. 61, p. 1-10, feb. 2019.
On-line 1678-9946
url http://www.repositorio.ufc.br/handle/riufc/46425
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1813028890864517120

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