Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos

Detalhes bibliográficos
Autor(a) principal: Aguiar, Bianca Dutra
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/67474
Resumo: The inflammatory process is related to increased resorption in some diseases, such as periodontitis, characterized by chronic inflammation in response to specific microorganisms that results in alveolar bone resorption (ROA) and tooth loss. Apigenin (APG), an agent found abundantly in chamomile, has received increased attention for its anti-inflammatory effects. Thus, the objective was to evaluate the antiresorptive activity of APG in experimental periodontitis in mice. For this, 48 male Swiss mice were used in ligation-induced periodontitis trials, which consists of the insertion of a cotton thread (4.0) around the lower first molars. The experimental groups consisted of: Naïve, in which periodontitis was not induced, untreated (NT) and APG, in which periodontitis was induced and received daily by gavage 10 µl/kg of TW80 and APG 1, 3 or 9 mg/ kg, respectively. After euthanasia, their mandibles were removed for analysis of ROA and attachment level (NI). Gingival tissue was also removed for quantification of IL-1β and IL-17, qRT-PCR for RANKL and OPG. Serum measurements of total alkaline phosphatase (FAT) and hepatic transaminases, analysis of renal (RI) and hepatic (HI) indices and body mass variation were performed. The ligation promoted a significant ROA, corroborated by the increase in the attachment level (NI) in the distal of the 1st molar and increase in the furcation area, as well as increasing the levels of IL-1β and IL-17 and the expression of RANKL in the gingival tissue. in NT mice compared to Naïve. On the other hand, the administration of APG (9 mg/kg) promoted a reduction in ROA, NI and the furcation area of the mandibular first molar, maintained the levels of IL-1β and IL-17 similar to those of Naïve and increased the expression of OPG Systemically, APG did not promote any significant change in the parameters evaluated. APG presented a bone protective profile, probably due to the control of disease progression through the modulation of osteoclastogenic cytokines, in addition to being systemically safe.
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spelling Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongosApigena reduces inflammatory bone resorption in a mouse model of periodontitisReabsorção ósseaPeriodontiteApigeninaThe inflammatory process is related to increased resorption in some diseases, such as periodontitis, characterized by chronic inflammation in response to specific microorganisms that results in alveolar bone resorption (ROA) and tooth loss. Apigenin (APG), an agent found abundantly in chamomile, has received increased attention for its anti-inflammatory effects. Thus, the objective was to evaluate the antiresorptive activity of APG in experimental periodontitis in mice. For this, 48 male Swiss mice were used in ligation-induced periodontitis trials, which consists of the insertion of a cotton thread (4.0) around the lower first molars. The experimental groups consisted of: Naïve, in which periodontitis was not induced, untreated (NT) and APG, in which periodontitis was induced and received daily by gavage 10 µl/kg of TW80 and APG 1, 3 or 9 mg/ kg, respectively. After euthanasia, their mandibles were removed for analysis of ROA and attachment level (NI). Gingival tissue was also removed for quantification of IL-1β and IL-17, qRT-PCR for RANKL and OPG. Serum measurements of total alkaline phosphatase (FAT) and hepatic transaminases, analysis of renal (RI) and hepatic (HI) indices and body mass variation were performed. The ligation promoted a significant ROA, corroborated by the increase in the attachment level (NI) in the distal of the 1st molar and increase in the furcation area, as well as increasing the levels of IL-1β and IL-17 and the expression of RANKL in the gingival tissue. in NT mice compared to Naïve. On the other hand, the administration of APG (9 mg/kg) promoted a reduction in ROA, NI and the furcation area of the mandibular first molar, maintained the levels of IL-1β and IL-17 similar to those of Naïve and increased the expression of OPG Systemically, APG did not promote any significant change in the parameters evaluated. APG presented a bone protective profile, probably due to the control of disease progression through the modulation of osteoclastogenic cytokines, in addition to being systemically safe.O processo inflamatório está relacionado ao aumento da reabsorção em algumas doenças, como a periodontite, caracterizada por uma inflamação crônica em resposta a micro-organismos específicos que resulta em reabsorção óssea alveolar (ROA) e perda do dente. A apigenina (APG), um agente encontrado abundantemente na camomila, tem recebido maior atenção por seus efeitos antiinflamatórios. Assim, objetivou-se avaliar a atividade antirreabsortiva da APG na periodontite experimental em camundongos. Para isso, 48 camundongos Swiss machos foram utilizados em ensaios de periodontite induzida por ligadura, que consiste na inserção de um fio de algodão (4.0) ao redor dos primeiros molares inferiores. Os grupos experimentais consistiram em: Naïve, em que a periodontite não foi induzida, não tratado (NT) e APG, nos quais a periodontite foi induzida e receberam diariamente por gavagem 10 µl/kg de TW80 e APG 1, 3 ou 9 mg/kg, respectivamente. Após a eutanásia, suas mandíbulas foram removidas para análises da ROA e nível de inserção (NI). O tecido gengival também foi removido para quantificação de IL-1β e IL-17, qRT-PCR para RANKL e OPG. Foram realizadas dosagens séricas de fosfatase alcalina total (FAT) e transaminases hepáticas, análises dos índices renal (IR) e hepático (IH) e da variação de massa corpórea. A ligadura promoveu significante ROA, corroborado pelo aumento do nível de inserção (NI) na distal do 1º molar inferior e aumento da área de furca, bem como aumentou os níveis de IL-1β e IL-17 e a expressão de RANKL no tecido gengival em camundongos NT em comparação ao Naïve. Em contrapartida, a administração de APG (9 mg/kg) promoveu redução da ROA, do NI e da área de furca do primeiro molar inferior, manteve os níveis de IL-1β e IL-17 semelhantes aos do Naïve e aumentou a expressão de OPG. Sistemicamente, a APG não promoveu qualquer alteração significante nos parâmetros avaliados. O APG apresentou um perfil protetor ósseo, provavelmente devido ao controle da progressão da doença através da modulação de citocinas osteoclastogênicas.Melo, Iracema Matos de MeloAguiar, Bianca Dutra2022-08-01T14:15:07Z2022-08-01T14:15:07Z2022-05-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfAGUIAR, B.D. Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos. 2022. 59 f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde - Campus de Sobral, Universidade Federal do Ceará, Sobral, 2022.http://www.repositorio.ufc.br/handle/riufc/67474porreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-08-01T14:15:08Zoai:repositorio.ufc.br:riufc/67474Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:28:36.644810Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
Apigena reduces inflammatory bone resorption in a mouse model of periodontitis
title Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
spellingShingle Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
Aguiar, Bianca Dutra
Reabsorção óssea
Periodontite
Apigenina
title_short Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
title_full Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
title_fullStr Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
title_full_unstemmed Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
title_sort Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos
author Aguiar, Bianca Dutra
author_facet Aguiar, Bianca Dutra
author_role author
dc.contributor.none.fl_str_mv Melo, Iracema Matos de Melo
dc.contributor.author.fl_str_mv Aguiar, Bianca Dutra
dc.subject.por.fl_str_mv Reabsorção óssea
Periodontite
Apigenina
topic Reabsorção óssea
Periodontite
Apigenina
description The inflammatory process is related to increased resorption in some diseases, such as periodontitis, characterized by chronic inflammation in response to specific microorganisms that results in alveolar bone resorption (ROA) and tooth loss. Apigenin (APG), an agent found abundantly in chamomile, has received increased attention for its anti-inflammatory effects. Thus, the objective was to evaluate the antiresorptive activity of APG in experimental periodontitis in mice. For this, 48 male Swiss mice were used in ligation-induced periodontitis trials, which consists of the insertion of a cotton thread (4.0) around the lower first molars. The experimental groups consisted of: Naïve, in which periodontitis was not induced, untreated (NT) and APG, in which periodontitis was induced and received daily by gavage 10 µl/kg of TW80 and APG 1, 3 or 9 mg/ kg, respectively. After euthanasia, their mandibles were removed for analysis of ROA and attachment level (NI). Gingival tissue was also removed for quantification of IL-1β and IL-17, qRT-PCR for RANKL and OPG. Serum measurements of total alkaline phosphatase (FAT) and hepatic transaminases, analysis of renal (RI) and hepatic (HI) indices and body mass variation were performed. The ligation promoted a significant ROA, corroborated by the increase in the attachment level (NI) in the distal of the 1st molar and increase in the furcation area, as well as increasing the levels of IL-1β and IL-17 and the expression of RANKL in the gingival tissue. in NT mice compared to Naïve. On the other hand, the administration of APG (9 mg/kg) promoted a reduction in ROA, NI and the furcation area of the mandibular first molar, maintained the levels of IL-1β and IL-17 similar to those of Naïve and increased the expression of OPG Systemically, APG did not promote any significant change in the parameters evaluated. APG presented a bone protective profile, probably due to the control of disease progression through the modulation of osteoclastogenic cytokines, in addition to being systemically safe.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-01T14:15:07Z
2022-08-01T14:15:07Z
2022-05-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv AGUIAR, B.D. Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos. 2022. 59 f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde - Campus de Sobral, Universidade Federal do Ceará, Sobral, 2022.
http://www.repositorio.ufc.br/handle/riufc/67474
identifier_str_mv AGUIAR, B.D. Apigena reduz reabsorção óssea inflamatória em modelo de periodontite em camundongos. 2022. 59 f. Dissertação (Mestrado em Ciências da Saúde) - Programa de Pós-Graduação em Ciências da Saúde - Campus de Sobral, Universidade Federal do Ceará, Sobral, 2022.
url http://www.repositorio.ufc.br/handle/riufc/67474
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dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
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reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
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