Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells

Detalhes bibliográficos
Autor(a) principal: Rádis-Baptista, Gandhi
Data de Publicação: 2012
Outros Autores: de la Torre, Beatriz G., Andreu, David
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/64586
Resumo: Nucleolar targeting peptides are 14–15 residue-longsequences designed by structural minimization of asnake toxin (J Med Chem 2008;50:7041). Peptidessuch as NrTP1 (YKQCHKKGGKK GSG) and ana-logues are capable of penetrating human cervix epi-thelial carcinoma cells and homing into theirnucleoli. We now show that NrTP1 similarly pene-trates and localizes in the nucleolus of tumour cellsderived from human pancreatic (BxPC-3) and humanductal mammary gland (BT-474) carcinomas. Livecell confocal microscopy imaging, combined withflow cytometry analysis of cells arrested to definedphases of their cycle, confirms that NrTP1 uptakeand nucleolar homing are independent of cell cyclephase. Peptide uptake is significantly reduced atlow temperature. Also, drugs inhibiting chlatrin-mediated endocytosis severely decrease uptake,pointing to a clathrin-dependent route as the pri-mary NrTP1 internalization mechanism. Theseresults highlight nucleolar targeting peptides notonly as a novel and efficient class of cell-penetratingpeptides but also for their exceptional ability to tar-get preferentially an essential and dynamic subnu-clear structure such as the nucleolus
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spelling Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cellsInsights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells.Cell penetrating peptideTumour cell targetCathrin mediated endocytosisPeptídioc - Penetração celularTumor - Célula - AlvoEndocitose - Mediador - CathrinNucleolar targeting peptides are 14–15 residue-longsequences designed by structural minimization of asnake toxin (J Med Chem 2008;50:7041). Peptidessuch as NrTP1 (YKQCHKKGGKK GSG) and ana-logues are capable of penetrating human cervix epi-thelial carcinoma cells and homing into theirnucleoli. We now show that NrTP1 similarly pene-trates and localizes in the nucleolus of tumour cellsderived from human pancreatic (BxPC-3) and humanductal mammary gland (BT-474) carcinomas. Livecell confocal microscopy imaging, combined withflow cytometry analysis of cells arrested to definedphases of their cycle, confirms that NrTP1 uptakeand nucleolar homing are independent of cell cyclephase. Peptide uptake is significantly reduced atlow temperature. Also, drugs inhibiting chlatrin-mediated endocytosis severely decrease uptake,pointing to a clathrin-dependent route as the pri-mary NrTP1 internalization mechanism. Theseresults highlight nucleolar targeting peptides notonly as a novel and efficient class of cell-penetratingpeptides but also for their exceptional ability to tar-get preferentially an essential and dynamic subnu-clear structure such as the nucleolusChemical Biology & Drug Design2022-03-24T14:09:09Z2022-03-24T14:09:09Z2012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRÁDIS-BAPTISTA, Gandhi; DE LA TORRE, Beatriz G.; ANDREU, David. Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells. Chemical Biology & Drug Design, United States of America, v. 79, p. 907-915, 2012.1747-0285http://www.repositorio.ufc.br/handle/riufc/64586Rádis-Baptista, Gandhide la Torre, Beatriz G.Andreu, Davidengreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-03-24T14:38:37Zoai:repositorio.ufc.br:riufc/64586Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:31:02.368328Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells.
title Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
spellingShingle Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
Rádis-Baptista, Gandhi
Cell penetrating peptide
Tumour cell target
Cathrin mediated endocytosis
Peptídioc - Penetração celular
Tumor - Célula - Alvo
Endocitose - Mediador - Cathrin
title_short Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
title_full Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
title_fullStr Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
title_full_unstemmed Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
title_sort Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells
author Rádis-Baptista, Gandhi
author_facet Rádis-Baptista, Gandhi
de la Torre, Beatriz G.
Andreu, David
author_role author
author2 de la Torre, Beatriz G.
Andreu, David
author2_role author
author
dc.contributor.author.fl_str_mv Rádis-Baptista, Gandhi
de la Torre, Beatriz G.
Andreu, David
dc.subject.por.fl_str_mv Cell penetrating peptide
Tumour cell target
Cathrin mediated endocytosis
Peptídioc - Penetração celular
Tumor - Célula - Alvo
Endocitose - Mediador - Cathrin
topic Cell penetrating peptide
Tumour cell target
Cathrin mediated endocytosis
Peptídioc - Penetração celular
Tumor - Célula - Alvo
Endocitose - Mediador - Cathrin
description Nucleolar targeting peptides are 14–15 residue-longsequences designed by structural minimization of asnake toxin (J Med Chem 2008;50:7041). Peptidessuch as NrTP1 (YKQCHKKGGKK GSG) and ana-logues are capable of penetrating human cervix epi-thelial carcinoma cells and homing into theirnucleoli. We now show that NrTP1 similarly pene-trates and localizes in the nucleolus of tumour cellsderived from human pancreatic (BxPC-3) and humanductal mammary gland (BT-474) carcinomas. Livecell confocal microscopy imaging, combined withflow cytometry analysis of cells arrested to definedphases of their cycle, confirms that NrTP1 uptakeand nucleolar homing are independent of cell cyclephase. Peptide uptake is significantly reduced atlow temperature. Also, drugs inhibiting chlatrin-mediated endocytosis severely decrease uptake,pointing to a clathrin-dependent route as the pri-mary NrTP1 internalization mechanism. Theseresults highlight nucleolar targeting peptides notonly as a novel and efficient class of cell-penetratingpeptides but also for their exceptional ability to tar-get preferentially an essential and dynamic subnu-clear structure such as the nucleolus
publishDate 2012
dc.date.none.fl_str_mv 2012
2022-03-24T14:09:09Z
2022-03-24T14:09:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv RÁDIS-BAPTISTA, Gandhi; DE LA TORRE, Beatriz G.; ANDREU, David. Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells. Chemical Biology & Drug Design, United States of America, v. 79, p. 907-915, 2012.
1747-0285
http://www.repositorio.ufc.br/handle/riufc/64586
identifier_str_mv RÁDIS-BAPTISTA, Gandhi; DE LA TORRE, Beatriz G.; ANDREU, David. Insights into the uptake mechanism of NrTP, A cell-penetrating peptide preferentially targetingthe nucleolus of tumour cells. Chemical Biology & Drug Design, United States of America, v. 79, p. 907-915, 2012.
1747-0285
url http://www.repositorio.ufc.br/handle/riufc/64586
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Chemical Biology & Drug Design
publisher.none.fl_str_mv Chemical Biology & Drug Design
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
_version_ 1813028837045305344

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