Toxicogenetic profile of rats treated with aqueous extract from Morinda citrifolia fruits

• Autor(a) principal: Moraes, Germano Pinho de
• Data de Publicação: 2016
• Outros Autores: Alencar, Marcus Vinícius Oliveira Barros de, Islam, Torequl, Araújo, Lidiane da Silva, Gomes, Débora Cássia Vieira, Carvalho, Rodrigo Mendes de, Corrêa, Dione, Paz, Márcia Fernanda Correia Jardim, Ferreira, Paulo Michel Pinheiro, Melo-Cavalcante, Ana Amélia de Carvalho, Ferraz, Alexandre, Grivicich, Ivana, Picada, Jaqueline Nascimento
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/18769
• topic: Genotoxicidade; Morinda

Morinda citrifolia ( (Family: Rubiaceae) ) is extensive ly use d in traditional med icine due to its anti - inflammatory, antimicrobial, antitumoral , and anti - hypertensive act ivities . However, t here is no substantial data about hepatotoxic and toxicogenetic effects . This study evaluated bioch emical changes and hepatotoxic, genotoxic , and mu tagenic effects of aqueous extract of the fruit of M. citrifolia (AEMC) in liver, bone marrow , and peripheral blood cells. A nimals ( Rattus novergicus , 5 males and 5 females) were divided into negative control , positive control ( C yclophosphamid e 25 mg/kg ) , and AEMC (2.5, 5 , and 10 mg/kg , by gavage ) . AEMC induced increase of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), especially at 10 mg/kg in female (174.8 ± 50.7, 221.4 ± 24.6 , and 174.7 ± 14.3 U/L) and m ale ( 156.5 ± 21.6, 183.7 ± 21.5 , and 147.3 ± 17.8 U/L) ( p <0.05). Histological analysis of livers showed inflammatory cell infiltrati on , nuclear fragmentation, microvacuolization, cellular swelling, points of inflammatoy necrosis , and discrete microvesicula r steatosis. DNA damage in hepatocytes w as found in both genders, mainly at 10 mg/kg (Frequency of Damage: 78.1 ± 4.5 and 70.4 ± 7.3%; Index of Damage: 107.6 ± 14.2 and 136.0 ± 26.9 for male and female, respectively). Similar results were observed in bone marrow cells . The AEMC 5 and 10 mg/kg induced micronucleus formation (4.4 ± 0.8 and 7.8 ± 1.1; 7.4 ± 1.1 and 9.6 ± 1.4 for peripheral blood and bone marrow cells, respectively) ( p <0.05). These findings suggest clastogenic and/or aneugenic effects and genet ic instability activated by AEMC , indicating precaution regarding the consumption of formulations or folk preparations based on this plant.