Production of hybrid nanoflowers with different lipase enzymes

• Autor(a) principal: Bezerra, Rayanne Mendes
• Data de Publicação: 2017
• Outros Autores: Rios, Nathalia Saraiva, Gomes, Pedro Henrique de Lima, Porto, Claudio Henrique Victor, Gonçalves, Luciana Rocha Barros, Santos, José Cleiton Sousa dos
• Tipo de documento: Artigo de conferência
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/54438
• topic: Enzimas; Imobilização de lipase; Lipases

The synthesis of the hybrid nanoflowers is a simple, low cost and environmental friendly enzyme immobilization method. In this synthesis, the enzyme catalytic activity do not decrease due to unfavorable conformation of the enzyme or to mass transfer problems. In this context, it was produced the hybrid nanoflowers of lipases from Candida antarctica type B (CALB) and from Thermomyces lanuginosus (TLL) and, posteriorly, the crosslinking were realized by glutaraldehyde (GA) and divinylsulfone (DVS), changing some conditions like: the concentration of the GA and DVS, time of reticulation and temperature of reticulation. The activity of the hybrid nanoflowers was quantified by p-nitrophenyl butyrate (pNPB) hydrolysis. The best result was obtained producing TLL hybrid nanoflowers by CuSO4 precipitation and DVS crosslinking (0.04 % v/v, at 25 ºC during 24 h) with retention of activity of 169.31 %. In addition, the CALB hybrid nanoflowers by CuCl2 precipitation and GA crosslinking (0.8 % v/v, at 4 ºC during 24 h) retained 120.27 % of its activity.