Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal do Ceará (UFC) |
dARK ID: | ark:/83112/0013000017z06 |
Texto Completo: | http://www.repositorio.ufc.br/handle/riufc/64156 |
Resumo: | The seeds of Prosopis juliflora (mesquite) have a water barrier composed of the palisade layer present in the seed tegument and galactomannans in the endosperm. Changes in the morphological and physiological states of seeds are usually accompanied by changes in their metabolism. The aim of this study was to detect and characterise the main reserves and their mobilisation during and following germination of P. juliflora seeds. Sections of seeds 0, 24, 48 and 72 h after the onset of the imbibing process were evaluated by light microscopy, and seed reserves were extracted and quantified. Polysaccharides were isolated and characterised by 1H nuclear magnetic resonance (NMR) spectroscopy. In mesquite seeds a well-developed endosperm, consisting largely of the storage carbohydrate (galactomannans), lies between the seed coat and the cotyledons. Germination was complete after 24 h of imbibition. At this time, morphological changes were observed in stained protein bodies. Chemical analysis showed a decrease in protein levels until 48 h. After 48 h, globular structures were observed in the cotyledon cells, but after 72 h these grains were smaller and their content was reduced. Polarisation microscopy confirmed that this material was composed of starch grains. The 1H NMR spectrum of P. juliflora seeds showed that sucrose was used following germination. Apparently, sucrose is the transport sugar mobilised from reserve stocks for seedling growth. Parallel to endosperm galactomannan degradation, starch is produced transitorily in the cotyledon. 2007 Society of Chemical Industry. |
id |
UFC-7_d3e541b600c7d53ec2cc78bdb4abd120 |
---|---|
oai_identifier_str |
oai:repositorio.ufc.br:riufc/64156 |
network_acronym_str |
UFC-7 |
network_name_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository_id_str |
|
spelling |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae)Prosopis julifloraGalactomannanMesquiteLeguminosaeThe seeds of Prosopis juliflora (mesquite) have a water barrier composed of the palisade layer present in the seed tegument and galactomannans in the endosperm. Changes in the morphological and physiological states of seeds are usually accompanied by changes in their metabolism. The aim of this study was to detect and characterise the main reserves and their mobilisation during and following germination of P. juliflora seeds. Sections of seeds 0, 24, 48 and 72 h after the onset of the imbibing process were evaluated by light microscopy, and seed reserves were extracted and quantified. Polysaccharides were isolated and characterised by 1H nuclear magnetic resonance (NMR) spectroscopy. In mesquite seeds a well-developed endosperm, consisting largely of the storage carbohydrate (galactomannans), lies between the seed coat and the cotyledons. Germination was complete after 24 h of imbibition. At this time, morphological changes were observed in stained protein bodies. Chemical analysis showed a decrease in protein levels until 48 h. After 48 h, globular structures were observed in the cotyledon cells, but after 72 h these grains were smaller and their content was reduced. Polarisation microscopy confirmed that this material was composed of starch grains. The 1H NMR spectrum of P. juliflora seeds showed that sucrose was used following germination. Apparently, sucrose is the transport sugar mobilised from reserve stocks for seedling growth. Parallel to endosperm galactomannan degradation, starch is produced transitorily in the cotyledon. 2007 Society of Chemical Industry.Journal of the Science of Food and Agriculture2022-02-24T12:23:38Z2022-02-24T12:23:38Z2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGALLAO, Maria I. et al. Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae). Journal of the Science of Food and Agriculture, [s. l.], v. 87, p. 2012-2018, 2007.http://www.repositorio.ufc.br/handle/riufc/64156ark:/83112/0013000017z06Gallao, Maria IzabelVieira, Ícaro G. P.Mendes, Francisca N. P.Souza, Alex S. N. deBrito, Edy S. deinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFC2023-10-10T19:33:36Zoai:repositorio.ufc.br:riufc/64156Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:27:05.037901Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false |
dc.title.none.fl_str_mv |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) |
title |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) |
spellingShingle |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) Gallao, Maria Izabel Prosopis juliflora Galactomannan Mesquite Leguminosae |
title_short |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) |
title_full |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) |
title_fullStr |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) |
title_full_unstemmed |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) |
title_sort |
Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae) |
author |
Gallao, Maria Izabel |
author_facet |
Gallao, Maria Izabel Vieira, Ícaro G. P. Mendes, Francisca N. P. Souza, Alex S. N. de Brito, Edy S. de |
author_role |
author |
author2 |
Vieira, Ícaro G. P. Mendes, Francisca N. P. Souza, Alex S. N. de Brito, Edy S. de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gallao, Maria Izabel Vieira, Ícaro G. P. Mendes, Francisca N. P. Souza, Alex S. N. de Brito, Edy S. de |
dc.subject.por.fl_str_mv |
Prosopis juliflora Galactomannan Mesquite Leguminosae |
topic |
Prosopis juliflora Galactomannan Mesquite Leguminosae |
description |
The seeds of Prosopis juliflora (mesquite) have a water barrier composed of the palisade layer present in the seed tegument and galactomannans in the endosperm. Changes in the morphological and physiological states of seeds are usually accompanied by changes in their metabolism. The aim of this study was to detect and characterise the main reserves and their mobilisation during and following germination of P. juliflora seeds. Sections of seeds 0, 24, 48 and 72 h after the onset of the imbibing process were evaluated by light microscopy, and seed reserves were extracted and quantified. Polysaccharides were isolated and characterised by 1H nuclear magnetic resonance (NMR) spectroscopy. In mesquite seeds a well-developed endosperm, consisting largely of the storage carbohydrate (galactomannans), lies between the seed coat and the cotyledons. Germination was complete after 24 h of imbibition. At this time, morphological changes were observed in stained protein bodies. Chemical analysis showed a decrease in protein levels until 48 h. After 48 h, globular structures were observed in the cotyledon cells, but after 72 h these grains were smaller and their content was reduced. Polarisation microscopy confirmed that this material was composed of starch grains. The 1H NMR spectrum of P. juliflora seeds showed that sucrose was used following germination. Apparently, sucrose is the transport sugar mobilised from reserve stocks for seedling growth. Parallel to endosperm galactomannan degradation, starch is produced transitorily in the cotyledon. 2007 Society of Chemical Industry. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2022-02-24T12:23:38Z 2022-02-24T12:23:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GALLAO, Maria I. et al. Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae). Journal of the Science of Food and Agriculture, [s. l.], v. 87, p. 2012-2018, 2007. http://www.repositorio.ufc.br/handle/riufc/64156 |
dc.identifier.dark.fl_str_mv |
ark:/83112/0013000017z06 |
identifier_str_mv |
GALLAO, Maria I. et al. Reserve mobilisation in mesquite (Prosopis juliflora) seed (Leguminosae). Journal of the Science of Food and Agriculture, [s. l.], v. 87, p. 2012-2018, 2007. ark:/83112/0013000017z06 |
url |
http://www.repositorio.ufc.br/handle/riufc/64156 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Journal of the Science of Food and Agriculture |
publisher.none.fl_str_mv |
Journal of the Science of Food and Agriculture |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal do Ceará (UFC) instname:Universidade Federal do Ceará (UFC) instacron:UFC |
instname_str |
Universidade Federal do Ceará (UFC) |
instacron_str |
UFC |
institution |
UFC |
reponame_str |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
collection |
Repositório Institucional da Universidade Federal do Ceará (UFC) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC) |
repository.mail.fl_str_mv |
bu@ufc.br || repositorio@ufc.br |
_version_ |
1818373919334203392 |