Depth-scanning confocal Raman for rapid in vivo determination of testosterone concentration profiles in human skin

• Autor(a) principal: Botelho, Marco Antonio
• Data de Publicação: 2014
• Outros Autores: Guerreiro, Stela Julia, Queiroz, Dinalva Brito, Barros, Gisele, Cavalcante, Monaliza, Souza, Juliana M. O., Silva, André M., Lemos, Telma L. G., Quintans Júnior, Lucindo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/9241
• topic: Nanotecnologia; Testosterona

OBJECTIVE: The present study aimed to measure in-vivo the depth and the percentage of testosterone in human skin. METHOD: Two healthy young Brazilian volunteers were evaluated through a Confocal Raman Spectroscopy probe on the right volar forearm. Testosterone spectroscopy of was performed on the Stratum Corneum, Viable Epidermis and Dermis; the percentage concentration of testosterone was compared between the baseline and one hour after local application of a transdermal nanostructured testosterone (5%) formulation. RESULTS: To the best of our knowledge, this is the first time that the depth and percentage of testosterone has been evaluated non-invasively in-vivo. No adverse events were attributed to this protocol. The method is effective in differentially measuring testosterone in the skin layers. CONCLUSION: This protocol may serve as a good choice for rapid hormone quantification for forensic or medical purposes.