Immunohistochemical expression of the tumor suppressor protein p16 INK4a in cervical adenocarcinoma

• Autor(a) principal: Eleutério Júnior, José
• Data de Publicação: 2017
• Outros Autores: Lima, Thiago Silva, Cunha, Maria do Perpétuo Socorro, Cavalcante, Diane Isabelle Magno, Silva, Angélica Maria Holanda
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• Texto Completo: http://www.scielo.br/scielo.php?script=sci_serial&pid=0100-7203&lng=pt&nrm=iso http://diadorim.ibict.br/handle/1/132; http://www.repositorio.ufc.br/handle/riufc/29687
• topic: Adenocarcinoma; Colo do Útero; Imuno-Histoquímica; Immunohistochemistry

Objective To evaluate the diagnostic utility of the p16ink4a protein expression as a marker for adenocarcinoma of the cervix. Methods In a cross-sectional study, p16ink4a expression was evaluated in 30 cervical biopsies from patients diagnosed with invasive adenocarcinoma from 2 reference clinics in Brazil, and compared with 18 biopsies of endocervical polyps (control cases). The performance of the tests for p16ink4a was evaluated using a conventional contingency table, and the Kappa (κ) index was used to evaluate the agreement of the marker with the tissue diagnosis. Results In total, 66% of the invasive adenocarcinoma cases were positive for p16ink4a. All of the adenomatous polyps cases used as negative controls were shown to be negative for p16ink4a. The marker showed a high sensitivity and a high negative predictive value. The Kappa index was good for p16ink4a (κ = 0.6). Conclusion Considering the strong association between the p16ink4a marker and the cervical adenocarcinoma, its use represents an important tool for reducing incorrect diagnoses of adenocarcinoma and thereby avoiding overtreatment.