Sensibilidade antimicrobiana e virulência de agentes bacterianos isolados do útero e da vagina de cadelas com piometra

• Autor(a) principal: Paiva, Débora Damásio de Queiroz
• Data de Publicação: 2020
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
• dARK ID: ark:/83112/0013000024qq0
• Texto Completo: http://www.repositorio.ufc.br/handle/riufc/56198
• topic: Fatores de Virulência; Cães; Testes de Sensibilidade Microbiana

Pyometra is one of the most common diseases in adult bitches, and is characterized by uterine endometrial hyperplasia and infiltration of inflammatory cells associated with bacterial infection. Several bacteria have already been associated with its development, such as Escherichia coli, Staphylococcus spp, Streptococcus spp, and Pseudomonas spp. This study aimed to identify bacteria present in the uterine content of bitches with pyometra and evaluate them for antimicrobial sensitivity and virulence factors production. For this purpose, samples of uterine and vaginal contents were collected from 30 bitches diagnosed with pyometra. The experiments followed the following steps: isolation and identification of bacterial isolates, evaluation of antimicrobials susceptibility, evaluation of biofilm, siderophores, proteases and hemolysins production in biofilm and planktonic form. A total of 81 bacterial isolates was identified, with the main genera isolated from the uterus and vagina being Escherichia 39.51% (32/81), Staphylococcus 17.27% (14/81) and Pseudomonas 9.87% (8/81). As for sensitivity, 88.5% (23/26) of Gram positive strains were resistant to at least one drug, while 60% (33/55) of Gram negative strains were resistant to at least one drug. Regarding virulence, it was observed that the isolates had low production of virulence factors. Thus, it is possible to infer that the occurrence of pyometra appears to be more associated with host factors than with bacterial factors.