Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil

Detalhes bibliográficos
Autor(a) principal: Lee, Lundoi Tobias
Data de Publicação: 2021
Outros Autores: Martinazzo, Ana Paula, Garcia, Sabrinna Aires, Berbet, Pedro Amorim, Teodoro, Carlos Eduardo de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Brasiliensis (Online)
Texto Completo: http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/528
Resumo: Fungi are one of the main food spoilage agents. Numerous species when subjected to stress conditions produce secondary metabolites known as mycotoxins, which are mutagenic and carcinogenic substances. The fungus Aspergillus flavus is one of the main contaminants of grains and is known to produce Aflatoxin. Pesticides are used in agriculture to contain fungi and other pests, but they harm other species, the environment and the human health, in addition to the development of resistance to these substances in pest species. Natural alternatives have been sought to control these organisms. In this context, essential oils are a viable option against A. flavus. The aim of this study was to identify the main components and evaluate the effectiveness of lemongrass essential oil (Cymbopogon flexuosus) for controlling the fungus Aspergillus flavus. Initially, the effect of essential oil on mycelial growth of the fungus was assessed by in vitro tests at the doses: 0.05; 0.1; 0.2; 0.4; 0.6; 0.8; 1.6; 3.2; 6.4; and 12.8 μL mL-1. The minimum inhibitory concentration (MIC) was 0.8 μL mL-1. The in vivo test was performed at the following concentrations: 0.6; 0.8; and 1.6 μL mL-1. The results showed that the essential oil has fungicidal potential against A. flavus. The main component of the essential oil was citral.
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spelling Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil Controle do Aspergillus flavus em grãos de trigo através do óleo essencial de Cymbopogon flexuosusFungi are one of the main food spoilage agents. Numerous species when subjected to stress conditions produce secondary metabolites known as mycotoxins, which are mutagenic and carcinogenic substances. The fungus Aspergillus flavus is one of the main contaminants of grains and is known to produce Aflatoxin. Pesticides are used in agriculture to contain fungi and other pests, but they harm other species, the environment and the human health, in addition to the development of resistance to these substances in pest species. Natural alternatives have been sought to control these organisms. In this context, essential oils are a viable option against A. flavus. The aim of this study was to identify the main components and evaluate the effectiveness of lemongrass essential oil (Cymbopogon flexuosus) for controlling the fungus Aspergillus flavus. Initially, the effect of essential oil on mycelial growth of the fungus was assessed by in vitro tests at the doses: 0.05; 0.1; 0.2; 0.4; 0.6; 0.8; 1.6; 3.2; 6.4; and 12.8 μL mL-1. The minimum inhibitory concentration (MIC) was 0.8 μL mL-1. The in vivo test was performed at the following concentrations: 0.6; 0.8; and 1.6 μL mL-1. The results showed that the essential oil has fungicidal potential against A. flavus. The main component of the essential oil was citral.O fungo Aspergillus flavus Link (1809) é um dos principais contaminantes de grãos e é conhecido por produzir a aflatoxina. O método para conter fungos e outras pragas na agricultura é com o uso de agrotóxicos, porém estes causam danos a outras espécies, ao meio ambiente e a saúde humana, além das pragas adquirirem resistências a essas substâncias. Tem-se buscado alternativas naturais para controlar esses organismos. Nesse contexto, os óleos essenciais se apresentam como opção. Este estudo identificou os principais componentes e avaliou a eficácia do óleo essencial de Cymbopogon flexuosus Stapf (capim limão) para controle do fungo A. flavus.  O efeito do óleo essencial sobre o crescimento micelial do fungo foi avaliado em testes in vitro nas doses entre 0,05 e 12,8 µL mL-1.  O teste in vivo foi realizado nas seguintes concentrações: 0,6; 0,8 e 1,6 µL mL-1. O óleo essencial, apresentou composição rica em citral (formado pelos isômeros geranial e neral). A concentração inibitória mínima (CIM) foi de 0,8 μL mL-1, apresentou potencial fungicida sobre o A. flavus, demonstrando ser promissor na substituição de agrotóxicos, sendo uma alternativa de baixo impacto para a saúde e o meio ambiente.Universidade Federal de Campina Grande - UFCG2021-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/52810.22571/2526-4338528Acta Brasiliensis; Vol 5 No 3 (2021): Acta Brasiliensis; 92-96Acta Brasiliensis; Vol 5 No 3 (2021): Acta Brasiliensis; 92-96Acta Brasiliensis; Vol 5 No 3 (2021): Acta Brasiliensis; 92-962526-43382526-432Xreponame:Acta Brasiliensis (Online)instname:Universidade Federal de Campina Grande (UFCG)instacron:UFCGenghttp://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/528/130Copyright (c) 2021 Acta Brasiliensisinfo:eu-repo/semantics/openAccessLee, Lundoi TobiasMartinazzo, Ana PaulaGarcia, Sabrinna AiresBerbet, Pedro AmorimTeodoro, Carlos Eduardo de Souza2021-09-30T20:53:56Zoai:ActaBra.revistas.ufcg.edu.br:article/528Revistahttp://revistas.ufcg.edu.br/ActaBraPUBhttp://revistas.ufcg.edu.br/ActaBra/index.php/actabra/oaiactabrasiliensis@gmail.com || actabrasiliensis@gmail.com2526-432X2526-4338opendoar:2021-09-30T20:53:56Acta Brasiliensis (Online) - Universidade Federal de Campina Grande (UFCG)false
dc.title.none.fl_str_mv Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil

Controle do Aspergillus flavus em grãos de trigo através do óleo essencial de Cymbopogon flexuosus
title Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil
spellingShingle Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil
Lee, Lundoi Tobias
title_short Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil
title_full Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil
title_fullStr Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil
title_full_unstemmed Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil
title_sort Control of Aspergillus flavus in wheat grains using Cymbopogon flexuosus essential oil
author Lee, Lundoi Tobias
author_facet Lee, Lundoi Tobias
Martinazzo, Ana Paula
Garcia, Sabrinna Aires
Berbet, Pedro Amorim
Teodoro, Carlos Eduardo de Souza
author_role author
author2 Martinazzo, Ana Paula
Garcia, Sabrinna Aires
Berbet, Pedro Amorim
Teodoro, Carlos Eduardo de Souza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lee, Lundoi Tobias
Martinazzo, Ana Paula
Garcia, Sabrinna Aires
Berbet, Pedro Amorim
Teodoro, Carlos Eduardo de Souza
description Fungi are one of the main food spoilage agents. Numerous species when subjected to stress conditions produce secondary metabolites known as mycotoxins, which are mutagenic and carcinogenic substances. The fungus Aspergillus flavus is one of the main contaminants of grains and is known to produce Aflatoxin. Pesticides are used in agriculture to contain fungi and other pests, but they harm other species, the environment and the human health, in addition to the development of resistance to these substances in pest species. Natural alternatives have been sought to control these organisms. In this context, essential oils are a viable option against A. flavus. The aim of this study was to identify the main components and evaluate the effectiveness of lemongrass essential oil (Cymbopogon flexuosus) for controlling the fungus Aspergillus flavus. Initially, the effect of essential oil on mycelial growth of the fungus was assessed by in vitro tests at the doses: 0.05; 0.1; 0.2; 0.4; 0.6; 0.8; 1.6; 3.2; 6.4; and 12.8 μL mL-1. The minimum inhibitory concentration (MIC) was 0.8 μL mL-1. The in vivo test was performed at the following concentrations: 0.6; 0.8; and 1.6 μL mL-1. The results showed that the essential oil has fungicidal potential against A. flavus. The main component of the essential oil was citral.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/528
10.22571/2526-4338528
url http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/528
identifier_str_mv 10.22571/2526-4338528
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://revistas.ufcg.edu.br/ActaBra/index.php/actabra/article/view/528/130
dc.rights.driver.fl_str_mv Copyright (c) 2021 Acta Brasiliensis
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Acta Brasiliensis
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Campina Grande - UFCG
publisher.none.fl_str_mv Universidade Federal de Campina Grande - UFCG
dc.source.none.fl_str_mv Acta Brasiliensis; Vol 5 No 3 (2021): Acta Brasiliensis; 92-96
Acta Brasiliensis; Vol 5 No 3 (2021): Acta Brasiliensis; 92-96
Acta Brasiliensis; Vol 5 No 3 (2021): Acta Brasiliensis; 92-96
2526-4338
2526-432X
reponame:Acta Brasiliensis (Online)
instname:Universidade Federal de Campina Grande (UFCG)
instacron:UFCG
instname_str Universidade Federal de Campina Grande (UFCG)
instacron_str UFCG
institution UFCG
reponame_str Acta Brasiliensis (Online)
collection Acta Brasiliensis (Online)
repository.name.fl_str_mv Acta Brasiliensis (Online) - Universidade Federal de Campina Grande (UFCG)
repository.mail.fl_str_mv actabrasiliensis@gmail.com || actabrasiliensis@gmail.com
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