Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFC |
Texto Completo: | http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14484 |
Resumo: | The Binder of Sperm Protein 1 (BSP1) is a protein produced by seminal vesicles of animals. It helps into the fertilization process of oocytes, promoting sperm capacitation, mediating the ligation from itself with the oviduct epithelium, and increasing the motility in the oviduct. When spermatozoa with purified BSP1 were incubated, acrosomal reaction rates were determined with and without the presence of heparin, as controls. The percentage of acrosomal reaction was higher (p <0.05) in control 1 (44.5%) compared with the other treatments (BSP1:10 Âg/mL: 24.5%; 20 Âg/mL: 25.5%; 40 Âg/mL: 26.0 %), and lower the rate (p> 0.05) when sperm were incubated only in Fert-TALP medium without heparin (14.5%). Cleavage and blastocyst rates were evaluated on days two and seven respectively, after fertilize oocytes in a medium containing BSP1. 1274 cumulus- oocyte complexes were recovered and incubated for 18 hours with frozen-thawed ejaculated semen in a Fert-TALP medium, containing: only heparin, 10, 20 or 40 μg/mL of BSP1. Cleavage and blastocyst rates (34.1  4.4 vs 40.8  5.0%) were similar (p> 0.05) when incubation was made with 10 Âg/mL of BSP1 and heparin, respectively. However, the concentrations of 20 and 40 Âg/mL decreased the formation of blastocysts (22.4  2.9 and 19.3  4.1%) compared with heparin (40.8  5.1%, p <0.02). Subsequently, cumulus- oocyte complexes (n = 2239) were incubated with frozen-thawed ejaculated semen. Spermatozoa were selected by a gradient of 45% -90% Percoll containing: only heparin, 10, 20, 40 μg/mL of BSP1 and there was a control 2 of Percoll without heparin or BSP1. Cleavage rate was similar (p> 0.05) for the incubation with concentrations of 10, 20 and 40 μg/mL of BPS1 (67.7  3.0, 68.7  3.5, 75.2  3.8, respectively). However, these rates were similar when it was used just heparin and when oocytes were incubated with 40Âg/mL of BSP1 (78.9  1.7 vs 75.2  3.8%). For blastocyst rates was found that they were similar (p> 0.05) when the selection was made with heparin (44.1  4.3%) and 40 Âg/mL of BSP1 (30.0  3.3%). Finally, 1213 cumulus-oocyte complexes were recovered and incubated with freeze-thawed epididymal semen in Fert-TALP medium, which contained: only heparin, with heparin, and 10, 20 or 40 Âg/mL of BSP1. Cleavage and blastocyst rates were similar after incubation with and without heparin, but the concentration of 40Âg/mL of BSP1 produced higher cleavages rates (79.0  1.1%) than control 1 (68.5  1.3%, p <0.05). About blastocyst rates, there were rates significantly higher when concentrations of 20 and 40 Âg/mL of BSP1 were used (35.6  2.5 and 41.1  2.0%) than with (24.7  3.2; p <0.05) and without heparin (27.3  1.6%; p <0.0003). In conclusion, BSP1 allowed proper cleavage and development, when the protein was added to the fertilization medium and the Percoll gradient using ejaculated semen, and to the fertilization medium when used epididymal sperm, without requiring the use of heparin. |
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info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisEffects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocystsAÃÃes da binder of Sperm protein 1 (BSP1) sobre a funcionalidade de espermatozoides bovinos: reaÃÃo acrossÃmica, taxa de clivagem e produÃÃo in vitro de blastocistos2015-02-13Arlindo de Alencar Araripe Noronha Moura28462874300http://lattes.cnpq.br/7244663767446774Jorge Andrà Matias Martins82316686320http://lattes.cnpq.br/9617318260953422Marcelo Bertolini49085662087http://lattes.cnpq.br/1311612572712450Paula Rodriguez Villamil84399694068http://lattes.cnpq.br/940401314918186370242514170http://lattes.cnpq.br/7556472165570453VerÃnica Hoyos MarulandaUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em ZootecniaUFCBRBSP1 Heparina FertilizaÃÃo in vitro Taxa de clivagem Taxa de blastocistosBSP1 Heparin In vitro fertilization Cleavage rate Blastocyst rateZOOTECNIAThe Binder of Sperm Protein 1 (BSP1) is a protein produced by seminal vesicles of animals. It helps into the fertilization process of oocytes, promoting sperm capacitation, mediating the ligation from itself with the oviduct epithelium, and increasing the motility in the oviduct. When spermatozoa with purified BSP1 were incubated, acrosomal reaction rates were determined with and without the presence of heparin, as controls. The percentage of acrosomal reaction was higher (p <0.05) in control 1 (44.5%) compared with the other treatments (BSP1:10 Âg/mL: 24.5%; 20 Âg/mL: 25.5%; 40 Âg/mL: 26.0 %), and lower the rate (p> 0.05) when sperm were incubated only in Fert-TALP medium without heparin (14.5%). Cleavage and blastocyst rates were evaluated on days two and seven respectively, after fertilize oocytes in a medium containing BSP1. 1274 cumulus- oocyte complexes were recovered and incubated for 18 hours with frozen-thawed ejaculated semen in a Fert-TALP medium, containing: only heparin, 10, 20 or 40 μg/mL of BSP1. Cleavage and blastocyst rates (34.1  4.4 vs 40.8  5.0%) were similar (p> 0.05) when incubation was made with 10 Âg/mL of BSP1 and heparin, respectively. However, the concentrations of 20 and 40 Âg/mL decreased the formation of blastocysts (22.4  2.9 and 19.3  4.1%) compared with heparin (40.8  5.1%, p <0.02). Subsequently, cumulus- oocyte complexes (n = 2239) were incubated with frozen-thawed ejaculated semen. Spermatozoa were selected by a gradient of 45% -90% Percoll containing: only heparin, 10, 20, 40 μg/mL of BSP1 and there was a control 2 of Percoll without heparin or BSP1. Cleavage rate was similar (p> 0.05) for the incubation with concentrations of 10, 20 and 40 μg/mL of BPS1 (67.7  3.0, 68.7  3.5, 75.2  3.8, respectively). However, these rates were similar when it was used just heparin and when oocytes were incubated with 40Âg/mL of BSP1 (78.9  1.7 vs 75.2  3.8%). For blastocyst rates was found that they were similar (p> 0.05) when the selection was made with heparin (44.1  4.3%) and 40 Âg/mL of BSP1 (30.0  3.3%). Finally, 1213 cumulus-oocyte complexes were recovered and incubated with freeze-thawed epididymal semen in Fert-TALP medium, which contained: only heparin, with heparin, and 10, 20 or 40 Âg/mL of BSP1. Cleavage and blastocyst rates were similar after incubation with and without heparin, but the concentration of 40Âg/mL of BSP1 produced higher cleavages rates (79.0  1.1%) than control 1 (68.5  1.3%, p <0.05). About blastocyst rates, there were rates significantly higher when concentrations of 20 and 40 Âg/mL of BSP1 were used (35.6  2.5 and 41.1  2.0%) than with (24.7  3.2; p <0.05) and without heparin (27.3  1.6%; p <0.0003). In conclusion, BSP1 allowed proper cleavage and development, when the protein was added to the fertilization medium and the Percoll gradient using ejaculated semen, and to the fertilization medium when used epididymal sperm, without requiring the use of heparin.A âBinder of Sperm Protein 1â (BSP1) à uma proteÃna produzida pelas vesÃculas seminais dos animais, que ajuda no processo de fertilizaÃÃo do oÃcito promovendo a capacitaÃÃo espermÃtica, mediando a sua ligaÃÃo com o epitÃlio do oviduto e aumentando sua motilidade no oviduto. As taxas de reaÃÃo de acrossoma dos espermatozoides foram determinadas ao serem incubados com BSP1 purificado, e com e sem a presenÃa de heparina, como controles. A porcentagem de reaÃÃo de acrossoma foi maior (p<0.05) no controle 1 (44.5%) comparado com os tratamentos restantes (BSP1: 10 Âg/mL: 24.5%; 20 Âg/mL: 25.5%; 40 Âg/mL: 26.0%), sendo menor esta taxa (p>0.05) quando os espermatozoides sà foram incubados no meio Fert-TALP sem heparina (14.5%). As taxas de clivagem e de blastocistos foram avaliadas nos dias dois e sete respectivamente, depois de fertilizar oÃcitos em um meio que continha BSP1. Foram recuperados 1274 COCs e incubados durante 18 horas com sÃmen ejaculado congelado- descongelado em meio Fert-TALP, que continha: sà heparina, 10, 20 ou 40 Âg/mL de BSP1. As taxas de clivagem e de blastocistos (34.1  4.4 vs 40.8  5.0%) foram similares (p>0.05) quando se fez a incubaÃÃo com 10 Âg/mL de BSP1 e heparina, respectivamente. PorÃm, as concentraÃÃes de 20 e 40 Âg/mL diminuÃram a formaÃÃo de blastocistos (22.4  2.9 e 19.3  4.1%) em relaÃÃo com a heparina (40.8  5.1%; p < 0.02). Posteriormente foram incubados COCs (n= 2239), com sÃmen ejaculado congelado-descongelado que foi selecionado por um gradiente de Percoll 45%-90% que continha: sà heparina, 10, 20 ou 40 Âg/mL de BPS1 e foi realizado um controle 2 de Percoll sem heparina nem BSP1. A taxa de clivagem foi similar (p > 0.05) para a incubaÃÃo com as concentraÃÃes de 10, 20 e 40 Âg/mL de BSP1 (67.7Â3.0, 68.7Â3.5, 75.2Â3.8). PorÃm, estas taxas foram similares quando sà foi usada heparina e quando se incubou com 40 Âg/mL de BSP1 (78.9Â1.7 vs 75.2Â3.8%). Para as taxas de blastocistos encontrou-se que foram similares (p > 0.05) quando se fez a seleÃÃo com heparina (44.1Â4.3%) e com 40 Âg/mL de BSP1 (30.0Â3.3%). Finalmente, foram recuperados 1213 complexos cumulus-oÃcitos e incubados com espermatozoides epididimÃrios congelados-descongelados em meio Fert-TALP, que continham: sà heparina, sem heparina, e 10, 20 ou 40 Âg/mL de BSP1. As taxas de clivagem e de blastocistos foram similares apÃs as incubaÃÃes com e sem heparina, mas a concentraÃÃo de 40 Âg/mL de BSP1 produziu maiores taxas de clivagem (79.0  1.1%) que o controle 1 (68.5  1.3%; p < 0.05). Quanto Ãs taxas de blastocistos, encontraram-se maiores taxas quando foram usadas as concentraÃÃes de 20 e 40 Âg/mL de BSP1 (35.6  2.5 e 41.1  2.0%) que com (24.7  3.2; p < 0.05) e sem heparina (27.3  1.6%; p < 0.0003). Em conclusÃo, a BSP1 adicionada ao meio de fertilizaÃÃo e ao gradiente de Percoll quando usado o sÃmen ejaculado, e ao meio de fertilizaÃÃo quando usados espermatozoides epididimÃrios, permitiu clivagem e desenvolvimento prÃprio, sem a necessidade do uso de heparina.CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superiorhttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14484application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:27:48Zmail@mail.com - |
dc.title.en.fl_str_mv |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts |
dc.title.alternative.pt.fl_str_mv |
AÃÃes da binder of Sperm protein 1 (BSP1) sobre a funcionalidade de espermatozoides bovinos: reaÃÃo acrossÃmica, taxa de clivagem e produÃÃo in vitro de blastocistos |
title |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts |
spellingShingle |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts VerÃnica Hoyos Marulanda BSP1 Heparina FertilizaÃÃo in vitro Taxa de clivagem Taxa de blastocistos BSP1 Heparin In vitro fertilization Cleavage rate Blastocyst rate ZOOTECNIA |
title_short |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts |
title_full |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts |
title_fullStr |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts |
title_full_unstemmed |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts |
title_sort |
Effects of Binder of SPerm protein 1 (BSP1) on bovine sperm function: acrosome reaction, cleavage rate and in vitro production of blastocysts |
author |
VerÃnica Hoyos Marulanda |
author_facet |
VerÃnica Hoyos Marulanda |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Arlindo de Alencar Araripe Noronha Moura |
dc.contributor.advisor1ID.fl_str_mv |
28462874300 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7244663767446774 |
dc.contributor.referee1.fl_str_mv |
Jorge Andrà Matias Martins |
dc.contributor.referee1ID.fl_str_mv |
82316686320 |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9617318260953422 |
dc.contributor.referee2.fl_str_mv |
Marcelo Bertolini |
dc.contributor.referee2ID.fl_str_mv |
49085662087 |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1311612572712450 |
dc.contributor.referee3.fl_str_mv |
Paula Rodriguez Villamil |
dc.contributor.referee3ID.fl_str_mv |
84399694068 |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9404013149181863 |
dc.contributor.authorID.fl_str_mv |
70242514170 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7556472165570453 |
dc.contributor.author.fl_str_mv |
VerÃnica Hoyos Marulanda |
contributor_str_mv |
Arlindo de Alencar Araripe Noronha Moura Jorge Andrà Matias Martins Marcelo Bertolini Paula Rodriguez Villamil |
dc.subject.por.fl_str_mv |
BSP1 Heparina FertilizaÃÃo in vitro Taxa de clivagem Taxa de blastocistos |
topic |
BSP1 Heparina FertilizaÃÃo in vitro Taxa de clivagem Taxa de blastocistos BSP1 Heparin In vitro fertilization Cleavage rate Blastocyst rate ZOOTECNIA |
dc.subject.eng.fl_str_mv |
BSP1 Heparin In vitro fertilization Cleavage rate Blastocyst rate |
dc.subject.cnpq.fl_str_mv |
ZOOTECNIA |
dc.description.sponsorship.fl_txt_mv |
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior |
dc.description.abstract.por.fl_txt_mv |
The Binder of Sperm Protein 1 (BSP1) is a protein produced by seminal vesicles of animals. It helps into the fertilization process of oocytes, promoting sperm capacitation, mediating the ligation from itself with the oviduct epithelium, and increasing the motility in the oviduct. When spermatozoa with purified BSP1 were incubated, acrosomal reaction rates were determined with and without the presence of heparin, as controls. The percentage of acrosomal reaction was higher (p <0.05) in control 1 (44.5%) compared with the other treatments (BSP1:10 Âg/mL: 24.5%; 20 Âg/mL: 25.5%; 40 Âg/mL: 26.0 %), and lower the rate (p> 0.05) when sperm were incubated only in Fert-TALP medium without heparin (14.5%). Cleavage and blastocyst rates were evaluated on days two and seven respectively, after fertilize oocytes in a medium containing BSP1. 1274 cumulus- oocyte complexes were recovered and incubated for 18 hours with frozen-thawed ejaculated semen in a Fert-TALP medium, containing: only heparin, 10, 20 or 40 μg/mL of BSP1. Cleavage and blastocyst rates (34.1  4.4 vs 40.8  5.0%) were similar (p> 0.05) when incubation was made with 10 Âg/mL of BSP1 and heparin, respectively. However, the concentrations of 20 and 40 Âg/mL decreased the formation of blastocysts (22.4  2.9 and 19.3  4.1%) compared with heparin (40.8  5.1%, p <0.02). Subsequently, cumulus- oocyte complexes (n = 2239) were incubated with frozen-thawed ejaculated semen. Spermatozoa were selected by a gradient of 45% -90% Percoll containing: only heparin, 10, 20, 40 μg/mL of BSP1 and there was a control 2 of Percoll without heparin or BSP1. Cleavage rate was similar (p> 0.05) for the incubation with concentrations of 10, 20 and 40 μg/mL of BPS1 (67.7  3.0, 68.7  3.5, 75.2  3.8, respectively). However, these rates were similar when it was used just heparin and when oocytes were incubated with 40Âg/mL of BSP1 (78.9  1.7 vs 75.2  3.8%). For blastocyst rates was found that they were similar (p> 0.05) when the selection was made with heparin (44.1  4.3%) and 40 Âg/mL of BSP1 (30.0  3.3%). Finally, 1213 cumulus-oocyte complexes were recovered and incubated with freeze-thawed epididymal semen in Fert-TALP medium, which contained: only heparin, with heparin, and 10, 20 or 40 Âg/mL of BSP1. Cleavage and blastocyst rates were similar after incubation with and without heparin, but the concentration of 40Âg/mL of BSP1 produced higher cleavages rates (79.0  1.1%) than control 1 (68.5  1.3%, p <0.05). About blastocyst rates, there were rates significantly higher when concentrations of 20 and 40 Âg/mL of BSP1 were used (35.6  2.5 and 41.1  2.0%) than with (24.7  3.2; p <0.05) and without heparin (27.3  1.6%; p <0.0003). In conclusion, BSP1 allowed proper cleavage and development, when the protein was added to the fertilization medium and the Percoll gradient using ejaculated semen, and to the fertilization medium when used epididymal sperm, without requiring the use of heparin. A âBinder of Sperm Protein 1â (BSP1) à uma proteÃna produzida pelas vesÃculas seminais dos animais, que ajuda no processo de fertilizaÃÃo do oÃcito promovendo a capacitaÃÃo espermÃtica, mediando a sua ligaÃÃo com o epitÃlio do oviduto e aumentando sua motilidade no oviduto. As taxas de reaÃÃo de acrossoma dos espermatozoides foram determinadas ao serem incubados com BSP1 purificado, e com e sem a presenÃa de heparina, como controles. A porcentagem de reaÃÃo de acrossoma foi maior (p<0.05) no controle 1 (44.5%) comparado com os tratamentos restantes (BSP1: 10 Âg/mL: 24.5%; 20 Âg/mL: 25.5%; 40 Âg/mL: 26.0%), sendo menor esta taxa (p>0.05) quando os espermatozoides sà foram incubados no meio Fert-TALP sem heparina (14.5%). As taxas de clivagem e de blastocistos foram avaliadas nos dias dois e sete respectivamente, depois de fertilizar oÃcitos em um meio que continha BSP1. Foram recuperados 1274 COCs e incubados durante 18 horas com sÃmen ejaculado congelado- descongelado em meio Fert-TALP, que continha: sà heparina, 10, 20 ou 40 Âg/mL de BSP1. As taxas de clivagem e de blastocistos (34.1  4.4 vs 40.8  5.0%) foram similares (p>0.05) quando se fez a incubaÃÃo com 10 Âg/mL de BSP1 e heparina, respectivamente. PorÃm, as concentraÃÃes de 20 e 40 Âg/mL diminuÃram a formaÃÃo de blastocistos (22.4  2.9 e 19.3  4.1%) em relaÃÃo com a heparina (40.8  5.1%; p < 0.02). Posteriormente foram incubados COCs (n= 2239), com sÃmen ejaculado congelado-descongelado que foi selecionado por um gradiente de Percoll 45%-90% que continha: sà heparina, 10, 20 ou 40 Âg/mL de BPS1 e foi realizado um controle 2 de Percoll sem heparina nem BSP1. A taxa de clivagem foi similar (p > 0.05) para a incubaÃÃo com as concentraÃÃes de 10, 20 e 40 Âg/mL de BSP1 (67.7Â3.0, 68.7Â3.5, 75.2Â3.8). PorÃm, estas taxas foram similares quando sà foi usada heparina e quando se incubou com 40 Âg/mL de BSP1 (78.9Â1.7 vs 75.2Â3.8%). Para as taxas de blastocistos encontrou-se que foram similares (p > 0.05) quando se fez a seleÃÃo com heparina (44.1Â4.3%) e com 40 Âg/mL de BSP1 (30.0Â3.3%). Finalmente, foram recuperados 1213 complexos cumulus-oÃcitos e incubados com espermatozoides epididimÃrios congelados-descongelados em meio Fert-TALP, que continham: sà heparina, sem heparina, e 10, 20 ou 40 Âg/mL de BSP1. As taxas de clivagem e de blastocistos foram similares apÃs as incubaÃÃes com e sem heparina, mas a concentraÃÃo de 40 Âg/mL de BSP1 produziu maiores taxas de clivagem (79.0  1.1%) que o controle 1 (68.5  1.3%; p < 0.05). Quanto Ãs taxas de blastocistos, encontraram-se maiores taxas quando foram usadas as concentraÃÃes de 20 e 40 Âg/mL de BSP1 (35.6  2.5 e 41.1  2.0%) que com (24.7  3.2; p < 0.05) e sem heparina (27.3  1.6%; p < 0.0003). Em conclusÃo, a BSP1 adicionada ao meio de fertilizaÃÃo e ao gradiente de Percoll quando usado o sÃmen ejaculado, e ao meio de fertilizaÃÃo quando usados espermatozoides epididimÃrios, permitiu clivagem e desenvolvimento prÃprio, sem a necessidade do uso de heparina. |
description |
The Binder of Sperm Protein 1 (BSP1) is a protein produced by seminal vesicles of animals. It helps into the fertilization process of oocytes, promoting sperm capacitation, mediating the ligation from itself with the oviduct epithelium, and increasing the motility in the oviduct. When spermatozoa with purified BSP1 were incubated, acrosomal reaction rates were determined with and without the presence of heparin, as controls. The percentage of acrosomal reaction was higher (p <0.05) in control 1 (44.5%) compared with the other treatments (BSP1:10 Âg/mL: 24.5%; 20 Âg/mL: 25.5%; 40 Âg/mL: 26.0 %), and lower the rate (p> 0.05) when sperm were incubated only in Fert-TALP medium without heparin (14.5%). Cleavage and blastocyst rates were evaluated on days two and seven respectively, after fertilize oocytes in a medium containing BSP1. 1274 cumulus- oocyte complexes were recovered and incubated for 18 hours with frozen-thawed ejaculated semen in a Fert-TALP medium, containing: only heparin, 10, 20 or 40 μg/mL of BSP1. Cleavage and blastocyst rates (34.1  4.4 vs 40.8  5.0%) were similar (p> 0.05) when incubation was made with 10 Âg/mL of BSP1 and heparin, respectively. However, the concentrations of 20 and 40 Âg/mL decreased the formation of blastocysts (22.4  2.9 and 19.3  4.1%) compared with heparin (40.8  5.1%, p <0.02). Subsequently, cumulus- oocyte complexes (n = 2239) were incubated with frozen-thawed ejaculated semen. Spermatozoa were selected by a gradient of 45% -90% Percoll containing: only heparin, 10, 20, 40 μg/mL of BSP1 and there was a control 2 of Percoll without heparin or BSP1. Cleavage rate was similar (p> 0.05) for the incubation with concentrations of 10, 20 and 40 μg/mL of BPS1 (67.7  3.0, 68.7  3.5, 75.2  3.8, respectively). However, these rates were similar when it was used just heparin and when oocytes were incubated with 40Âg/mL of BSP1 (78.9  1.7 vs 75.2  3.8%). For blastocyst rates was found that they were similar (p> 0.05) when the selection was made with heparin (44.1  4.3%) and 40 Âg/mL of BSP1 (30.0  3.3%). Finally, 1213 cumulus-oocyte complexes were recovered and incubated with freeze-thawed epididymal semen in Fert-TALP medium, which contained: only heparin, with heparin, and 10, 20 or 40 Âg/mL of BSP1. Cleavage and blastocyst rates were similar after incubation with and without heparin, but the concentration of 40Âg/mL of BSP1 produced higher cleavages rates (79.0  1.1%) than control 1 (68.5  1.3%, p <0.05). About blastocyst rates, there were rates significantly higher when concentrations of 20 and 40 Âg/mL of BSP1 were used (35.6  2.5 and 41.1  2.0%) than with (24.7  3.2; p <0.05) and without heparin (27.3  1.6%; p <0.0003). In conclusion, BSP1 allowed proper cleavage and development, when the protein was added to the fertilization medium and the Percoll gradient using ejaculated semen, and to the fertilization medium when used epididymal sperm, without requiring the use of heparin. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
status_str |
publishedVersion |
format |
masterThesis |
dc.identifier.uri.fl_str_mv |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14484 |
url |
http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=14484 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.publisher.program.fl_str_mv |
Programa de PÃs-GraduaÃÃo em Zootecnia |
dc.publisher.initials.fl_str_mv |
UFC |
dc.publisher.country.fl_str_mv |
BR |
publisher.none.fl_str_mv |
Universidade Federal do Cearà |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFC instname:Universidade Federal do Ceará instacron:UFC |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFC |
collection |
Biblioteca Digital de Teses e Dissertações da UFC |
instname_str |
Universidade Federal do Ceará |
instacron_str |
UFC |
institution |
UFC |
repository.name.fl_str_mv |
-
|
repository.mail.fl_str_mv |
mail@mail.com |
_version_ |
1643295205629100032 |