Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin

Detalhes bibliográficos
Autor(a) principal: Jorgiana Silva de Assis
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFC
Texto Completo: http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169
Resumo: The aim of this study was to evaluate the efficacy of the flavonoid epigallocatechin-3-gallate (EGCG) in concentrations of 0.5%, 1% and 2% as an antimicrobial solution in artificially carious dentin, with 2% chlorhexidine and 0.9% saline solution as controls. Twenty-five slabs of human dentin (4 mm x 4 mm) were immersed for five days in Brain Heart Infusion broth (BHI-broth) inoculated at first day with Streptococcus mutans UA159 (Batch Culture Model). On the fifth day of the experiment, the blocks were randomly divided into five groups: group I - negative control - 0.9% saline solution, group II - positive control - 2% chlorhexidine, group III - 0.5% EGCG, group IV - 1% EGCG, group V - 2% EGCG. Each slab was subjected to 15 Âl of the tested solution for 60 seconds. After treatments, artificially carious dentin was removed from the dentin slabs and analyzed by counting colony forming units (CFUs). All experiments were performed in triplicate and the data obtained in CFUs mean values converted to log base-10. The statistical tests used were analysis of variance (ANOVA) followed by Tukey test. There was no statistical difference between EGCG concentrations and saline (p > 0.05). Furthermore, there is no statistical difference between EGCG concentrations (p > 0.05). However, there was statistically significant difference between chlorhexidine and the other groups (p < 0.05). From the data obtained in this study, is possible to conclude that the substance tested is not effective on elimination of pathogen S. mutans when used in a concentration of 0.5%, 1% and 2% in artificially carious dentin.
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spelling info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAnalysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentinAnÃlise antimicrobiana do flavonÃide epigalocatequina-3-galato como agente de limpeza cavitÃria em dentina artificialmente cariada2013-07-25SÃrgio Lima Santiago38299500397http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4705306Y9Cristiane SÃ Roriz Fonteles42747597334http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4723888D6ClÃudio Maniglia Ferreira14262563880http://lattes.cnpq.br/1944193921428069 01496584350 http://lattes.cnpq.br/0074589039823248Jorgiana Silva de AssisUniversidade Federal do CearÃPrograma de PÃs-GraduaÃÃo em OdontologiaUFCBRCatechin Dentin Streptococcus mutansODONTOLOGIAThe aim of this study was to evaluate the efficacy of the flavonoid epigallocatechin-3-gallate (EGCG) in concentrations of 0.5%, 1% and 2% as an antimicrobial solution in artificially carious dentin, with 2% chlorhexidine and 0.9% saline solution as controls. Twenty-five slabs of human dentin (4 mm x 4 mm) were immersed for five days in Brain Heart Infusion broth (BHI-broth) inoculated at first day with Streptococcus mutans UA159 (Batch Culture Model). On the fifth day of the experiment, the blocks were randomly divided into five groups: group I - negative control - 0.9% saline solution, group II - positive control - 2% chlorhexidine, group III - 0.5% EGCG, group IV - 1% EGCG, group V - 2% EGCG. Each slab was subjected to 15 Âl of the tested solution for 60 seconds. After treatments, artificially carious dentin was removed from the dentin slabs and analyzed by counting colony forming units (CFUs). All experiments were performed in triplicate and the data obtained in CFUs mean values converted to log base-10. The statistical tests used were analysis of variance (ANOVA) followed by Tukey test. There was no statistical difference between EGCG concentrations and saline (p > 0.05). Furthermore, there is no statistical difference between EGCG concentrations (p > 0.05). However, there was statistically significant difference between chlorhexidine and the other groups (p < 0.05). From the data obtained in this study, is possible to conclude that the substance tested is not effective on elimination of pathogen S. mutans when used in a concentration of 0.5%, 1% and 2% in artificially carious dentin.O objetivo do presente estudo foi avaliar a eficÃcia do flavonÃide epigalocatequina-3-galato (EGCG) nas concentraÃÃes de 0,5%, 1% e 2% como soluÃÃo antimicrobiana em dentina artificialmente cariada, tendo a clorexidina 2% e a soluÃÃo salina 0,9% como controles. Vinte e cinco blocos de dentina humana (4 mm x 4 mm) foram imersos por cinco dias em BHI-caldo inoculado com Streptococcus mutans UA159 no primeiro dia do experimento. No quinto dia do experimento, os blocos foram aleatoriamente distribuÃdos em cinco grupos: grupo I â controle negativo â soluÃÃo salina 0,9%; grupo II â controle positivo â clorexidina 2%; grupo III â EGCG 0,5%; grupo IV â EGCG 1%; grupo V - EGCG 2%. Cada bloco recebeu tratamento de 15 Âl da soluÃÃo testada, que permaneceu em contato com o bloco por 60 segundos. ApÃs os tratamentos, amostras dentinÃrias foram removidas com emprego de lÃmina de bisturi e foram analisadas a partir da contagem de UFCs (unidades formadoras de colÃnias). Os experimentos foram realizados em triplicata e os dados, obtidos em UFCs foram convertidos em log base-10. Os testes estatÃsticos empregados foram anÃlise de variÃncia (ANOVA), seguida de Teste de Tukey. NÃo houve diferenÃa estatÃstica entre as concentraÃÃes de EGCG empregadas e a soluÃÃo salina (p > 0,05). AlÃm disso, nÃo houve diferenÃa estatÃstica entre as concentraÃÃes de EGCG (p > 0,05). No entanto, houve diferenÃa estatisticamente significativa entre a clorexidina e os demais grupos (p < 0,05). Conclui-se, a partir dos dados encontrados, que a substÃncia investigada nÃo deve ser empregada em preparos cavitÃrios com a finalidade de eliminaÃÃo de patÃgenos, visto que nÃo se mostrou eficaz como antimicrobiano nas concentraÃÃes testadas.Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicohttp://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169application/pdfinfo:eu-repo/semantics/openAccessporreponame:Biblioteca Digital de Teses e Dissertações da UFCinstname:Universidade Federal do Cearáinstacron:UFC2019-01-21T11:24:19Zmail@mail.com -
dc.title.en.fl_str_mv Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
dc.title.alternative.pt.fl_str_mv AnÃlise antimicrobiana do flavonÃide epigalocatequina-3-galato como agente de limpeza cavitÃria em dentina artificialmente cariada
title Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
spellingShingle Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
Jorgiana Silva de Assis
Catechin
Dentin
Streptococcus mutans
ODONTOLOGIA
title_short Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
title_full Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
title_fullStr Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
title_full_unstemmed Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
title_sort Analysis of antimicrobial flavonoid epigallocatechin-3-gallate as a cavity cleansing agent in artificially carious dentin
author Jorgiana Silva de Assis
author_facet Jorgiana Silva de Assis
author_role author
dc.contributor.advisor1.fl_str_mv SÃrgio Lima Santiago
dc.contributor.advisor1ID.fl_str_mv 38299500397
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4705306Y9
dc.contributor.referee1.fl_str_mv Cristiane SÃ Roriz Fonteles
dc.contributor.referee1ID.fl_str_mv 42747597334
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.jsp?id=K4723888D6
dc.contributor.referee2.fl_str_mv ClÃudio Maniglia Ferreira
dc.contributor.referee2ID.fl_str_mv 14262563880
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/1944193921428069
dc.contributor.authorID.fl_str_mv 01496584350
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0074589039823248
dc.contributor.author.fl_str_mv Jorgiana Silva de Assis
contributor_str_mv SÃrgio Lima Santiago
Cristiane SÃ Roriz Fonteles
ClÃudio Maniglia Ferreira
dc.subject.eng.fl_str_mv Catechin
Dentin
Streptococcus mutans
topic Catechin
Dentin
Streptococcus mutans
ODONTOLOGIA
dc.subject.cnpq.fl_str_mv ODONTOLOGIA
dc.description.sponsorship.fl_txt_mv Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
dc.description.abstract.por.fl_txt_mv The aim of this study was to evaluate the efficacy of the flavonoid epigallocatechin-3-gallate (EGCG) in concentrations of 0.5%, 1% and 2% as an antimicrobial solution in artificially carious dentin, with 2% chlorhexidine and 0.9% saline solution as controls. Twenty-five slabs of human dentin (4 mm x 4 mm) were immersed for five days in Brain Heart Infusion broth (BHI-broth) inoculated at first day with Streptococcus mutans UA159 (Batch Culture Model). On the fifth day of the experiment, the blocks were randomly divided into five groups: group I - negative control - 0.9% saline solution, group II - positive control - 2% chlorhexidine, group III - 0.5% EGCG, group IV - 1% EGCG, group V - 2% EGCG. Each slab was subjected to 15 Âl of the tested solution for 60 seconds. After treatments, artificially carious dentin was removed from the dentin slabs and analyzed by counting colony forming units (CFUs). All experiments were performed in triplicate and the data obtained in CFUs mean values converted to log base-10. The statistical tests used were analysis of variance (ANOVA) followed by Tukey test. There was no statistical difference between EGCG concentrations and saline (p > 0.05). Furthermore, there is no statistical difference between EGCG concentrations (p > 0.05). However, there was statistically significant difference between chlorhexidine and the other groups (p < 0.05). From the data obtained in this study, is possible to conclude that the substance tested is not effective on elimination of pathogen S. mutans when used in a concentration of 0.5%, 1% and 2% in artificially carious dentin.
O objetivo do presente estudo foi avaliar a eficÃcia do flavonÃide epigalocatequina-3-galato (EGCG) nas concentraÃÃes de 0,5%, 1% e 2% como soluÃÃo antimicrobiana em dentina artificialmente cariada, tendo a clorexidina 2% e a soluÃÃo salina 0,9% como controles. Vinte e cinco blocos de dentina humana (4 mm x 4 mm) foram imersos por cinco dias em BHI-caldo inoculado com Streptococcus mutans UA159 no primeiro dia do experimento. No quinto dia do experimento, os blocos foram aleatoriamente distribuÃdos em cinco grupos: grupo I â controle negativo â soluÃÃo salina 0,9%; grupo II â controle positivo â clorexidina 2%; grupo III â EGCG 0,5%; grupo IV â EGCG 1%; grupo V - EGCG 2%. Cada bloco recebeu tratamento de 15 Âl da soluÃÃo testada, que permaneceu em contato com o bloco por 60 segundos. ApÃs os tratamentos, amostras dentinÃrias foram removidas com emprego de lÃmina de bisturi e foram analisadas a partir da contagem de UFCs (unidades formadoras de colÃnias). Os experimentos foram realizados em triplicata e os dados, obtidos em UFCs foram convertidos em log base-10. Os testes estatÃsticos empregados foram anÃlise de variÃncia (ANOVA), seguida de Teste de Tukey. NÃo houve diferenÃa estatÃstica entre as concentraÃÃes de EGCG empregadas e a soluÃÃo salina (p > 0,05). AlÃm disso, nÃo houve diferenÃa estatÃstica entre as concentraÃÃes de EGCG (p > 0,05). No entanto, houve diferenÃa estatisticamente significativa entre a clorexidina e os demais grupos (p < 0,05). Conclui-se, a partir dos dados encontrados, que a substÃncia investigada nÃo deve ser empregada em preparos cavitÃrios com a finalidade de eliminaÃÃo de patÃgenos, visto que nÃo se mostrou eficaz como antimicrobiano nas concentraÃÃes testadas.
description The aim of this study was to evaluate the efficacy of the flavonoid epigallocatechin-3-gallate (EGCG) in concentrations of 0.5%, 1% and 2% as an antimicrobial solution in artificially carious dentin, with 2% chlorhexidine and 0.9% saline solution as controls. Twenty-five slabs of human dentin (4 mm x 4 mm) were immersed for five days in Brain Heart Infusion broth (BHI-broth) inoculated at first day with Streptococcus mutans UA159 (Batch Culture Model). On the fifth day of the experiment, the blocks were randomly divided into five groups: group I - negative control - 0.9% saline solution, group II - positive control - 2% chlorhexidine, group III - 0.5% EGCG, group IV - 1% EGCG, group V - 2% EGCG. Each slab was subjected to 15 Âl of the tested solution for 60 seconds. After treatments, artificially carious dentin was removed from the dentin slabs and analyzed by counting colony forming units (CFUs). All experiments were performed in triplicate and the data obtained in CFUs mean values converted to log base-10. The statistical tests used were analysis of variance (ANOVA) followed by Tukey test. There was no statistical difference between EGCG concentrations and saline (p > 0.05). Furthermore, there is no statistical difference between EGCG concentrations (p > 0.05). However, there was statistically significant difference between chlorhexidine and the other groups (p < 0.05). From the data obtained in this study, is possible to conclude that the substance tested is not effective on elimination of pathogen S. mutans when used in a concentration of 0.5%, 1% and 2% in artificially carious dentin.
publishDate 2013
dc.date.issued.fl_str_mv 2013-07-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
status_str publishedVersion
format masterThesis
dc.identifier.uri.fl_str_mv http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169
url http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=11169
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.publisher.program.fl_str_mv Programa de PÃs-GraduaÃÃo em Odontologia
dc.publisher.initials.fl_str_mv UFC
dc.publisher.country.fl_str_mv BR
publisher.none.fl_str_mv Universidade Federal do CearÃ
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFC
instname:Universidade Federal do Ceará
instacron:UFC
reponame_str Biblioteca Digital de Teses e Dissertações da UFC
collection Biblioteca Digital de Teses e Dissertações da UFC
instname_str Universidade Federal do Ceará
instacron_str UFC
institution UFC
repository.name.fl_str_mv -
repository.mail.fl_str_mv mail@mail.com
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